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Preparation and application of novel haemagglutination antigen used for preventing infection with avian influenza virus

A technology of avian influenza virus and hemagglutinin, which is applied in application, antiviral agents, and medical preparations containing active ingredients, etc., can solve the problems of undisclosed new hemagglutinin antigens, and achieve easy process amplification and large-scale production, low cost effect

Inactive Publication Date: 2015-06-17
NINGBO XINAN MEDICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

H10N8 avian influenza virus is a new type of avian influenza virus. The first case was found in Jiangxi Province. Influenza A can be divided into HxNx (H is hemagglutinin protein, N is neuraminidase protein) according to the characteristics of influenza virus. There are more than 140 subtypes. At present, there are no relevant research reports on the preparation and application of new hemagglutinin antigens for the prevention of H10N8 avian influenza virus infection at home and abroad.

Method used

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  • Preparation and application of novel haemagglutination antigen used for preventing infection with avian influenza virus
  • Preparation and application of novel haemagglutination antigen used for preventing infection with avian influenza virus

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Embodiment Construction

[0014] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

1. Specific examples

[0015] Cloning of the hemagglutinin H10 gene of avian influenza virus H10N8 in recombinant baculovirus (rBV) for the preparation of SVP. The resulting rBV was used to express the H10 hemagglutinin protein in Sf9 cells. The SVP of H10 recombinant hemagglutinin isolated from Sf9 cells was purified by ion exchange (IEX) and molecular sieve chromatography after treatment with NP40 surfactant. The result is as figure 1 with figure 2 As shown, the virus particles are clear and of high purity.

[0016] 2. Application examples

[0017] We evaluated the immunogenicity and efficacy of recombinant hemagglutinin H10 in a challenging model of murine and ferret models. Immunogenicity was confirmed by HAI (hemagglutinin inhibition) and virus-neutralizing antibodies. Mortality was determined from vaccinated animals after three vi...

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Abstract

The invention discloses preparation and application of a novel hemagglutinin antigen used for preventing infection with the avian influenza virus. The preparation of the novel hemagglutinin antigen is characterized by comprising the following steps: (1) preparation of stable recombinant baculovirus (rBV) containing gene H10; (2) preparation of SVP of H10 expressed in Sf9 cells; and (3) purification and stabilization of SVP. The H10 recombinant hemagglutinin can be used for preventing infection with the avian influenza virus H10N8 and immunizing human beings or animals so as to produce antibodies and exert protective effects, and reacts with the antibodies against hemagglutinin H10N8 or H10; the H10 recombinant hemagglutinin has the characteristics of high efficiency and low cost and can be used for preventing the infection with the avian influenza virus H10N8; moreover, the recombinant hemagglutinin can be expressed in insect cells and does not depend on chicken embryos, so process scale-up and mass production of the recombinant hemagglutinin can be easily realized.

Description

technical field [0001] The invention relates to the preparation of a hemagglutinin antigen, in particular to the preparation and application of a novel hemagglutinin antigen for preventing avian influenza virus infection. Background technique [0002] Bird flu and seasonal flu are problems that plague the world. Infection of the latest H10N8 avian influenza virus to humans is an urgent problem to be solved. [0003] Pandemic influenza is a more serious illness than seasonal flu. For example, the 1918 "Spanish" flu caused 40 to 100 million deaths worldwide. The 2009-2010 H1N1 swine flu spread rapidly around the world and was defined by the WHO as the first major flu in the 21st century. Highly pathogenic avian influenza (HPAI) viruses may cause future pandemics. For example, the increasing number of cases of H5N1 avian influenza virus infection has also shown the possibility of causing a pandemic. Other potential pandemics include the H7N2 and H9N2 avian influenza viruse...

Claims

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Application Information

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IPC IPC(8): C12N15/866C12N7/04A61K39/145A61P31/16
Inventor 乔素梅
Owner NINGBO XINAN MEDICAL SCI & TECH
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