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Construction of smart surfaces with reversible control of cell adhesion under electrical control and a method for reversible control of cell adhesion by voltage

An electrical control, cell technology, applied in animal cells, tumor/cancer cells, vertebrate cells, etc., can solve problems such as the influence of RGD peptide on cell adhesion behavior.

Active Publication Date: 2017-08-22
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, no prior work has investigated the effect of conformational changes of the RGD peptide on cell adhesion behavior via charge-responsive smart surfaces.

Method used

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  • Construction of smart surfaces with reversible control of cell adhesion under electrical control and a method for reversible control of cell adhesion by voltage
  • Construction of smart surfaces with reversible control of cell adhesion under electrical control and a method for reversible control of cell adhesion by voltage
  • Construction of smart surfaces with reversible control of cell adhesion under electrical control and a method for reversible control of cell adhesion by voltage

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Experimental program
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Effect test

Embodiment 1

[0052] theoretical simulation

[0053] In order to confirm the effectiveness of the invention, it was proved that the positive charge on the RGD peptide with quaternary ammonium salt group at the end is mainly concentrated at the end of the molecule, so that only the end of the molecule can be repelled or attracted when a voltage is applied , a theoretical simulation of the molecular electrostatic potential was performed on the molecule. We simulated the molecular electrostatic potential of the RGD peptide with a quaternary ammonium salt at the end and the RGD peptide with an amino group at the end, and the simulation results are as follows figure 2 shown. From the results, we can see that, compared with the RGD peptide with an amino terminal, the electropositiveness of the RGD peptide with a quaternary ammonium salt at the end is very strong, and it is mainly concentrated in the terminal region where the quaternary ammonium salt is located. This has just guaranteed the the...

Embodiment 2

[0055] Construction of smart surfaces for reversible control of cell adhesion under electrical control

[0056] First, the evaporated gold sheet (2cm × 0.5cm, first evaporated a layer of 5nm thick chromium on the glass sheet, and then evaporated a layer of 100nm gold) was rinsed three times in secondary water and ethanol respectively to remove the gold. Dust and organic matter on the surface of the film were blown dry with argon and set aside.

[0057] Configure 5ml of mixed ethanol solution of RGD peptide and PEG, wherein the quality of RGD peptide is 0.04mg, and the concentration is 10 -5 mol / L, the volume of PEG is 13.2 μL, and the concentration is 99×10 -4 mol / L, argon to remove oxygen, stored in a 10ml centrifuge tube. Soak the treated gold flakes in the solution, seal them, and let them stand for 48 hours. During these 48 hours, the RGD peptide molecules and PEG molecules in the solution were self-assembled on the surface of the gold sheet through the gold-sulfur bond...

Embodiment 3

[0065] Construction of smart surfaces for reversible control of cell adhesion under electrical control

[0066] First, the evaporated silver sheet (2cm × 0.5cm, first evaporated a layer of 5nm thick chromium on the glass sheet, and then evaporated a layer of 100nm silver) was rinsed three times in secondary water and ethanol respectively to remove the silver. Dust and organic matter on the surface of the film were blown dry with argon and set aside.

[0067] Configure 5ml of mixed ethanol solution of RGD peptide and PEG, wherein the quality of RGD peptide is 0.04mg, and the concentration is 10 -5 mol / L, the volume of PEG is 13.2 μL, and the concentration is 99×10 -4 mol / L, argon to remove oxygen, stored in a 10ml centrifuge tube. Soak the treated silver flakes in the solution, seal it, and let it stand for 12 hours. Within 12 hours, the RGD peptide molecules and PEG molecules in the solution self-assembled on the surface of the silver sheet according to the ratio in the sol...

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Abstract

The invention provides a method for constructing an intelligent surface that reversibly controls cell adhesion under electrical control and a method for reversibly controlling cell adhesion through voltage. The construction method is as follows: immerse a gold sheet, silver sheet, platinum sheet or copper sheet in the RGD peptide with a charged group connected at the end and a group with a terminal group that can self-assemble with gold, silver, platinum or copper. In the mixed solution of polyethylene glycol derivatives, the assembled metal sheet is obtained through self-assembly in an inert atmosphere, and the smart surface with reversible control of cell adhesion under electrical control is completed. The present invention uses the principle of mutual repulsion between the same charges and mutual attraction of opposite charges to add a charged group to the end of the RGD peptide for non-specific adhesion to cells to make it compatible with the anti-cell adhesion polyethylene glycol derivatives and Mixed in a certain proportion, a self-assembled monomolecular film is formed on the surface of gold, silver, platinum or copper to realize the construction of a smart surface that reversibly controls cell adhesion under electrical control, and can be used to reversibly control cell adhesion through voltage.

Description

technical field [0001] The invention belongs to the field of biotechnology for dynamically controlling cell adhesion on surfaces, and relates to a method for constructing an intelligent surface for reversibly controlling cell adhesion under electrical control and a method for reversibly controlling cell adhesion through voltage, in particular to a method for reversibly controlling cell adhesion. The construction method of the attached smart surface and the method of reversibly controlling the adhesion of cells on the self-assembled monomolecular film on the surface of the smart surface, that is, the gold, silver, platinum, copper electrodes, the method is through the gold, silver, platinum, copper electrodes This is achieved by applying different voltages to the surface. Background technique [0002] "Smart" surfaces are functional surfaces that can respond to changes in external light, electricity, and temperature. They have broad application prospects in the fields of sens...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C23C26/00C12N5/071C12N5/09
Inventor 蒋兴宇李君雷祎凤郑文富
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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