Ionic liquid regulation-control bio-enzyme, method for degrading environmental hormone carbaryl by same, and method for determining degradation rate

A technology of ionic liquids and environmental hormones, applied in biochemical equipment and methods, enzyme stabilization, chemical instruments and methods, etc., can solve the problems of expensive membrane modules, high cost of chemical methods, incomplete treatment, etc., and achieve fast degradation rate , high catalytic efficiency, and the effect of improving the effective utilization rate

Inactive Publication Date: 2015-06-24
SHAANXI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the removal rate of membrane separation technology is relatively good, but the treatment process is not only related to the properties of the target object, but also related to the properties of the membrane and the composition of the solution, and the price of the membrane module is relatively expensive, and there are often clogging and The state of swelling, so it is difficult to widely apply membrane separation technology in wastewater treatment
The physical adsorption method has relatively high cost, incomplete treatment and many uncertainties in the treatment process.
Although using chemical oxidation method to degrade carbaryl can degrade organic matter more efficiently, non-selectively and relatively completely, new impurities will inevitably be introduced in the process of treatment, which will cause a burden to the environment; and the cost of chemical method is relatively high. There are still some difficulties in the whole process of industrial wastewater treatment

Method used

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  • Ionic liquid regulation-control bio-enzyme, method for degrading environmental hormone carbaryl by same, and method for determining degradation rate
  • Ionic liquid regulation-control bio-enzyme, method for degrading environmental hormone carbaryl by same, and method for determining degradation rate
  • Ionic liquid regulation-control bio-enzyme, method for degrading environmental hormone carbaryl by same, and method for determining degradation rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Take a pH value of 5.8 and a concentration of 0.1mol·L -1 KH 2 PO 4 -K 2 HPO 4 The solution was adjusted to a phosphate buffer solution with a pH of 2.75 with hydrochloric acid.

[0034] (2) Press Carvine and H 2 O 2 The ratio of the amount of the substance is 1: (9-12), and the initial concentration of 500μL is 100μmol·L -1 Carbaryl solution, 5μL initial concentration is 4.8μmol·L -1 Chloroperoxidase solution, 36μL initial concentration is 10mol·L -1 Tetramethylammonium bromide solution, and 45~60μL with an initial concentration of 10mmol·L -1 H 2 O 2 The solutions were added to the phosphate buffer solution, and the volume was adjusted to 3mL with the phosphate buffer solution, and mixed uniformly to form a reaction system. In the reaction system, the concentration of each substance is as follows: the concentration of chloroperoxidase is 0.008μmol·L -1 , The concentration of tetramethylammonium bromide is 0.12mol·L -1 , H 2 O 2 The concentration is 0.15~0.2mmol·L -1 .

...

Embodiment 2

[0047] (1) Take a pH value of 5.8 and a concentration of 0.1mol·L -1 KH 2 PO 4 -K 2 HPO 4 The solution is adjusted to a phosphate buffer solution with a pH of 2 with hydrochloric acid.

[0048] (2) Press Carvine and H 2 O 2 The amount ratio of the substance is 1:6, and the initial concentration of 500μL is 100μmol·L -1 Carbaryl solution, 5μL initial concentration is 30μmol·L -1 Chloroperoxidase solution, 60μL initial concentration is 10mol·L -1 Tetramethylammonium bromide solution, and 30μL initial concentration of 10mmol·L -1 H 2 O 2 The solutions were added to the phosphate buffer solution, and the volume was adjusted to 3 mL with the phosphate buffer solution, and mixed uniformly to form a reaction system. In the reaction system, the concentration of each substance is as follows: the concentration of chloroperoxidase is 0.05 μmol·L -1 , The concentration of tetramethylammonium bromide is 0.20mol·L -1 , H 2 O 2 The concentration is 0.2mmol·L -1 .

[0049] (3) Protected from light f...

Embodiment 3

[0052] (1) Take a pH value of 5.8 and a concentration of 0.1mol·L -1 KH 2 PO 4 -K 2 HPO 4 The solution is adjusted to a phosphate buffer solution with a pH of 2.5 with hydrochloric acid.

[0053] (2) Press Carvine and H 2 O 2 The quantity ratio of the substance is 1:3, and the initial concentration of 500μL is 100μmol·L -1 Carbaryl solution, 5μL initial concentration is 6μmol·L -1 Chloroperoxidase solution, 18μL initial concentration is 10mol·L -1 Tetraethylammonium bromide solution, and 15μL initial concentration of 10mmol·L -1 H 2 O 2 The solutions were added to the phosphate buffer solution, and the volume was adjusted to 3 mL with the phosphate buffer solution, and mixed uniformly to form a reaction system. In the reaction system, the concentration of each substance is as follows: the concentration of chloroperoxidase is 0.01 μmol·L -1 , The concentration of tetraethylammonium bromide is 0.06mol·L -1 , H 2 O 2 The concentration is 0.05mmol·L -1 .

[0054] (3) Protected from light...

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Abstract

The invention relates to an ionic liquid regulation-control bio-enzyme, a method for degrading environmental hormone carbaryl by the same, and a method for determining the degradation rate. The ionic liquid regulation-control bio-enzyme comprises the mixed solution formed by adding chloroperoxidase, quaternary ammonium salt ionic liquids and H2O2 in a phosphate buffer solution with a pH value of 2-5, wherein in the mixed solution, the concentration of chloroperoxidase is 0.005-0.05mu mol.L<-1>, the concentration of ammonium salt ionic liquids is 0.06-0.20mol.L<-1>, and the concentration of H2O2 is 0.05-0.5mmol.L<-1>. According to the ionic liquid regulation-control bio-enzyme, the method for degrading environmental hormone carbaryl by the same, and the method for determining the degradation rate disclosed by the invention, the chloroperoxidase is used as a biocatalyst, the ammonium salt ionic liquids are used as regulation-control factors, the ammonium salt ionic liquids have a cosolvent effect on the chloroperoxidase, and can be used for improving the stability of the chloroperoxidase, increasing the enzyme catalysis speed and increasing the effective utilization rate of the chloroperoxidase; the ionic liquid regulation-control bio-enzyme is capable of effectively improving the solubility of carbaryl and increasing the degradation rate in case of being applied to degradation for carbaryl.

Description

Technical field: [0001] The invention relates to the field of biodegradation, in particular to an ionic liquid regulating biological enzyme and a method for degrading the environmental hormone carbaryl and a method for determining the degradation rate. Background technique: [0002] In the 1950s, carbamate pesticides represented by carbaryl (CBL) entered the market and became the second main force following organophosphorus pesticides. Carbaryl, also known as carbaryl or carbaryl, has good residual effects and systemic properties, has contact and stomach toxicity to pests, is a broad-spectrum insecticide, and is widely used in fruit trees , Vegetables, grains, medicinal materials, tea and other crops. However, as its use becomes more and more widespread, its toxic effects are increasingly exposed. Especially in recent years, it has been found to have an interference effect on the human endocrine system. It is an environmental hormone substance (EDS) that can damage the reproduct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96C12N9/08A62D3/02C02F3/34G01N30/02A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28C02F3/342C02F2101/306C02F2101/38C12N9/0065C12N9/96C12Y111/01G01N30/02G01N2030/027
Inventor 蒋育澄王圣洁胡满成李淑妮翟全国
Owner SHAANXI NORMAL UNIV
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