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A strain of Bacillus cereus derived from deep sea and its application in the production of neutral protease

A Bacillus cereus and deep-sea technology, applied in the field of protease production strains, can solve the problems of low degree of development and ineffective development, and achieve the effects of easy cultivation, simple cultivation conditions and stable genetic properties

Active Publication Date: 2018-04-13
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Marine biological resources are rich, covering 70% of the earth's surface area, including 80% of the biological resources, and the degree of development is relatively low. The microorganisms that have been studied and identified are less than 5% of the total. The active substances that have been found Less than 1% of the total, far from being effectively developed, it has become a hot spot for development and utilization

Method used

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  • A strain of Bacillus cereus derived from deep sea and its application in the production of neutral protease
  • A strain of Bacillus cereus derived from deep sea and its application in the production of neutral protease
  • A strain of Bacillus cereus derived from deep sea and its application in the production of neutral protease

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Embodiment Construction

[0016] The implementation of the present invention will be further described below in conjunction with examples, but the implementation and inclusion of the present invention are not limited thereto. It should be pointed out that if there is a special detailed description below, those skilled in the art can realize it with reference to the prior art .

[0017] Enrichment and seed medium: peptone 5g, yeast powder 1g, artificial seawater 1L, pH 7.4-7.6.

[0018] Casein medium: 10g casein, 3g beef extract powder, 2g potassium dihydrogen phosphate, 15g agar powder, 1L artificial seawater, pH 7.4 or so.

[0019] Fermentation medium: glycerin 3g, glucose 5g, yeast powder 10g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.3g, ammonium sulfate 1g, calcium chloride 1g, sea salt 10g, pH7.2.

[0020] Isolation and screening of protease-producing CGMCC No. 8424

[0021] Enrichment culture: Weigh 2g of deep-sea mud sample into a 50mL sterilized centrifuge tube, add 15mL of ste...

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Abstract

The invention relates to a bacillus cereus derived from deep sea and its application in producing neutral protease. The Bacillus cereus SWJS 13 of the present invention was deposited on November 1, 2013 in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No.8424. The invention optimizes the fermentation condition of the neutral protease produced by the bacterial strain, and the culture condition is simple, easy to control, short fermentation period, high protease activity and stable genetic property.

Description

technical field [0001] The present invention relates to the protease producing bacterial strain of deep-sea source, be specifically related to Bacillus cereus ( Bacillus cereus SWJS 13) and its liquid fermentation method for producing protease. Background technique [0002] Protease is an enzyme preparation widely used in food, feed, chemical, pharmaceutical, leather and other industries. Different proteases have different application conditions and specificity of action. Only by selecting the appropriate protease can the ideal effect be obtained. With the diversification of protease industrial application conditions and the exploration of application potential in new fields, researchers have always maintained a high level of concern in exploring new protease resources and high-yielding protease strains. Marine biological resources are rich, covering 70% of the earth's surface area, including 80% of the biological resources, and the degree of development is relatively lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/54C12R1/085
CPCC12N9/54C12N1/205C12R2001/085
Inventor 赵谋明雷芬芬赵强忠崔春
Owner SOUTH CHINA UNIV OF TECH