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Cynanchum paniculatum (Bge.) Kitag. C21 steroid converted product and application thereof

A transformation product, Xu Changqing's technology, applied in the field of medicine, can solve problems such as no high content, no research reports on multidrug resistance reversal agents in tumor cells, etc.

Inactive Publication Date: 2015-07-08
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been reports in the literature on the isolation and identification of C21 steroidal compounds from Xu Changqing, but there is no product with a high content of Xu Changqing's C21 steroid conversion product; there is no information about the use of Xu Changqing's C21 steroid conversion product in the preparation of tumor cell multidrug resistance reversal agents research report

Method used

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  • Cynanchum paniculatum (Bge.) Kitag. C21 steroid converted product and application thereof
  • Cynanchum paniculatum (Bge.) Kitag. C21 steroid converted product and application thereof
  • Cynanchum paniculatum (Bge.) Kitag. C21 steroid converted product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Method for Determination of C21 Steroid Aglycon Weight Percentage in Xu Changqing C21 Steroid Transformation Products by Colorimetry

[0038] Solution preparation

[0039] Preparation of reference solution

[0040] Accurately weigh an appropriate amount of albino aglycone C reference substance, add methanol to make each 1 ml containing 186.32 mu g solution, as the reference solution.

[0041] Preparation of the test solution

[0042] Take 0.1 g of Xu Changqing C21 steroid conversion product, accurately weigh it, add methanol to dissolve, transfer to a 50 ml measuring bottle, add methanol to the mark, shake well, accurately draw 10 ml, put it in a 100 ml measuring bottle, add methanol to the mark , Shake well, filter, and take the continued filtrate as the test solution.

[0043] Assay method

[0044] Precisely draw the test solution 100 mu l, put it in a stoppered test tube, evaporate the solvent, add 5 ml of freshly prepared concentrated sulfuri...

Embodiment 2

[0046] Take 300 Kg of dried root and rhizome powder of Xu Changqing (produced in Shandong), add 90% ethanol to reflux and extract three times, each time the dosage is 2400 L, each extraction time is 2 hours, filter in several times, the extracts are combined, and recovered under reduced pressure Ethanol, the obtained extract was dissolved in water, chromatographed on a macroporous adsorption resin column D101, filled with a macroporous adsorption resin D101 to a volume of 150 L, eluted with water until the eluate was nearly colorless, discarded the eluent, and washed with 30% ethanol Elute until the eluent is nearly colorless, discard the eluent, elute with 70% ethanol until the eluent is nearly colorless, collect the eluate, and perform column chromatography on the decolorized resin D941 that has been treated, fill the volume of decolorized resin D941 150 L, elute with 900 L of 90% ethanol, collect the eluate, concentrate under reduced pressure, and dry to obtain the eluate, a...

Embodiment 3

[0048]Take 300 Kg of dried root and rhizome powder of Xu Changqing (produced in Guangxi), add 90% ethanol to reflux and extract three times, each time the dosage is 2400 L, each extraction time is 2 hours, filter in several times, the extracts are combined, and recovered under reduced pressure Ethanol, the obtained extract was dissolved in water, chromatographed on a macroporous adsorption resin column D101, filled with a macroporous adsorption resin D101 to a volume of 150 L, eluted with water until the eluate was nearly colorless, discarded the eluent, and washed with 30% ethanol Elute until the eluent is nearly colorless, discard the eluent, elute with 70% ethanol until the eluent is nearly colorless, collect the eluate, and perform column chromatography on the decolorized resin D941 that has been treated, fill the volume of decolorized resin D941 150 L, elute with 900 L of 90% ethanol, collect the eluate, concentrate under reduced pressure, and dry to obtain the eluate, add...

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Abstract

The invention provides a Cynanchum paniculatum (Bge.) Kitag. C21 steroid converted product and application of the converted product to preparation of a tumor cell multidrug-resistant reversal agent. The weight percentage content of C21 steroid aglycon in the converted product is greater than or equal to 90%. The converted product is used for reversing the resistance to common clinic drugs, including paclitaxel, adriamycin, 5-fluorouracil and cis-platinum, of human stomach cancer multidrug-resistant cells, human colon cancer multidrug-resistant cells and human lung cancer multidrug-resistant cells. The invention further provides a preparation method of the Cynanchum paniculatum (Bge.) Kitag. C21 steroid converted product. The preparation method is simple and convenient. The Cynanchum paniculatum (Bge.) Kitag. C21 steroid converted product is applied clinically to combination with antitumor drugs to improve the curative effect of chemotherapy, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to Xu Changqing's C21 steroid conversion product and its application in preparing tumor cell multidrug resistance reversal agents, and also relates to a preparation method of Xu Changqing's C21 steroid conversion product. Background technique [0002] Malignant tumor is one of the diseases that seriously endanger human health. It is characterized by abnormal cell proliferation and metastasis. Statistics from the World Health Organization show that in 2000, there were about 10 million new cases of malignant tumors in the world, 6.2 million deaths, and 22 million cases of illness; it is estimated that by 2020, there will be 15 million new cases of malignant tumors, 10 million deaths, and 30 million illnesses. Thousands of cases. Chemotherapy is one of the methods of comprehensive therapy for malignant tumors. It is widely used before, during and after surgery. However, the phenomenon of multidr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/27A61P35/00A61K125/00
Inventor 王威刘小红褚文希高华韩立春
Owner QINGDAO UNIV
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