A new type of freeze-dried treatment solution for platelets
A technology of platelets and treatment liquid, applied in the directions of microorganisms, biochemical equipment and methods, tissue culture, etc., can solve the problems of product collapse, low mechanical strength, product separation from the wall, shrinkage, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0061] 1) Prepare the platelet freezing intervention treatment solution: add 50mmol / L trehalose and 1ug / mL PGE1 to the basic buffer solution, adjust the pH to 6.6-6.8, filter through a 0.22um filter and set aside.
[0062] 2) Prepare platelet freeze-drying buffer: add 50mmol / L trehalose, 1ug / mL PGE1 and 30% v / v plasma to the basic buffer, adjust the pH to 6.6-6.8, filter through a 0.22um filter and set aside.
[0063] 3) Intervention of platelet freezing:
[0064] 1 part of pooled platelets was centrifuged at 1500×g for 15 minutes, the supernatant was discarded and the precipitate was retained, the platelets were resuspended with the same volume of the discarded supernatant as the platelet freezing pretreatment solution, and the platelets were kept in a suspended state at 37°C for 4 hours in a shaking water bath. After the water bath, centrifuge the platelet suspension at 1500×g for 15 min, discard the supernatant and save the precipitate, and resuspend the platelets with the ...
Embodiment 2
[0069] 1) Prepare the platelet freezing intervention treatment solution: add 50mmol / L trehalose and 1ug / mL PGE1 to the basic buffer solution, adjust the pH to 6.6-6.8, filter through a 0.22um filter and set aside.
[0070] 2) Prepare platelet freeze-drying buffer: add 50mmol / L trehalose, 1ug / mL PGE1 and 40% v / v plasma to the basic buffer, adjust the pH to 6.6-6.8, filter through a 0.22um filter and set aside.
[0071] 3) Intervention of platelet freezing:
[0072] 1 part of pooled platelets was centrifuged at 1500×g for 15 minutes, the supernatant was discarded and the precipitate was retained, the platelets were resuspended with the same volume of the discarded supernatant as the platelet freezing pretreatment solution, and the platelets were kept in a suspended state at 37°C for 4 hours in a shaking water bath. After the water bath, centrifuge the platelet suspension at 1500×g for 15 min, discard the supernatant and save the precipitate, and resuspend the platelets with the ...
PUM
Property | Measurement | Unit |
---|---|---|
thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com