Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for obtaining bioactive substance composition through immortalized cells

A biologically active substance and immortalization technology, applied in the field of immortalized cells, can solve the problems of loss of three-dimensional structure and weak activity, and achieve the effects of beautifying the skin, removing scars, and inhibiting the proliferation of keratinocytes

Inactive Publication Date: 2015-07-22
张烨
View PDF8 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are still some differences between the cytokines obtained by artificial chemical synthesis or genetic recombination and the cytokines produced by the human body. For example, these cytokines have more or less allergens, lose their specific three-dimensional structure, and have weak activity.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for obtaining bioactive substance composition through immortalized cells
  • Method for obtaining bioactive substance composition through immortalized cells
  • Method for obtaining bioactive substance composition through immortalized cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 The acquisition of immortalized B lymphocytes

[0063] By drawing venous blood, separate lymphocytes and produce immortalized cells by EBV transfection:

[0064] Somatic cells are most easily obtained from blood. Blood drawing is almost the least harmful in routine testing. Compared with other methods of obtaining somatic cells, blood drawing does not require traumatic surgery, professional technicians and equipment, and causes the least harm to the individual. In addition, the cost of blood drawing is relatively low, the operation is simple, the technology is mature, and the required materials are easy to obtain. Obtaining lymphocytes from blood is the best way to obtain somatic cells. During the collection process, 5-10ml of individual venous blood is collected by qualified medical personnel into heparin anticoagulated blood collection tubes. Delivered to the laboratory within 48 hours at room temperature for the next step.

[0065] The Epstein-Barr viru...

Embodiment 2

[0067] Example 2, Expansion and cultivation of immortalized B lymphocytes in serum-free medium

[0068] In order to obtain a large amount of biologically active substances, it is necessary to expand the culture of immortalized B lymphocytes. The cell culture process applied to the human body has high culture requirements, and serum-free medium with defined components is generally used. In the present invention, it is preferred to use serum-free medium (lymphocyte serum-free medium from Japan TAKARA Company or limited chemical composition serum-free medium from LONZA Company in the United States), when the number of immortalized cells reaches 3-6×10∧6 / cm3, centrifuge, wash with PBS buffer three times, add serum-free medium, and change to a larger culture bottle for cultivation as needed. In order to obtain more biologically active substances, one glass bead (Glass beads, acid-washed (425-600μm)) needs to be added to each culture well; recombinant human EFG (Shanghai Haohai Bi...

Embodiment 3

[0069] Embodiment 3, separation and purification of biologically active substances through a hollow fiber ultrafiltration system;

[0070] The immortalized B lymphocyte culture fluid was centrifuged at 5000rmp for 20min for solid-liquid separation, the supernatant was taken, and the supernatant was first ultrafiltered with a hollow fiber ultrafiltration system with a molecular weight cut-off of 100KD, the filtrate was collected, and then filtered with a molecular weight cut-off Perform ultrafiltration for the 50KD hollow fiber ultrafiltration system, and collect the concentrated filtrate. After the above process, most of the impurities in the medium can be removed, and relatively pure biologically active substances produced by immortal cells can be obtained, and then the concentrated solution can be directly subjected to the next production process according to needs, or the concentrated solution can be vacuum freeze-dried to prepare Cell-forming bioactive substance concentrat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing a composition containing human bioactive substances. The bioactive substances are from immortalized cells. The method concretely comprises the following steps: acquiring the immortalized cells made by using autologous cells, and obtaining the bioactive substance composition in a proper medium under proper culture conditions. The bioactive substance composition can be used in customized cosmetics as a raw material or additive in order to realize beauty treatment and skin maintenance. The bioactive substance composition in a cosmetic composition is from human body cells, has consistent three-dimensional structure and bioactivity with bioactive substances in the human body, has certain safety and good skin compatibility, can effectively obtain cell growth factors and other bioactive substances, and can improve cell collagen synthesis, promote fibroblast proliferation and inhibit UV light induced keratinocyte proliferation. The cosmetic composition is expected to be used for resisting wrinkles, beautifying skins, healing wounds and removing scars.

Description

technical field [0001] The invention relates to a manufacturing method of a composition containing human bioactive substances, in particular, the human bioactive substances are derived from immortalized cells. Specifically, the present invention provides an immortalized cell made from collected autologous cells, which can be used to obtain a human bioactive substance composition under appropriate medium and culture conditions, and the obtained human bioactive substance composition can be used for Personalized cosmetics for beauty and skin care. Background technique [0002] Cells derived from normal tissues grow and divide under normal in vitro culture conditions, and after a limited number of cell passages, they stop proliferating, appear senescent and die. Immortalized cells are cells that have achieved immortalization, and have the ability to divide and proliferate indefinitely, such as germ cells, stem cells, tumor cells, and immortalized cells constructed by transfecti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/85C12N15/869C12N5/10A61K8/66A61K8/64A61Q19/00
Inventor 不公告发明人
Owner 张烨
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com