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A detection primer and method for moire grouper microsatellite marker epinmoss

A technology of moire grouper and microsatellite marking, which is applied in biochemical equipment and methods, microbe measurement/testing, DNA/RNA fragments, etc., can solve problems such as limiting the development of molecular genetics, and achieve the effect of simple methods

Active Publication Date: 2017-09-05
临沂小鲁生物科技有限公司
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Problems solved by technology

However, due to the lack of microsatellite markers of grouper moire, the development of its molecular genetics research is limited, so it is urgent to obtain microsatellite markers for the research and analysis of genetic diversity, individual identification and pedigree authentication of grouper moire. application

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  • A detection primer and method for moire grouper microsatellite marker epinmoss

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Embodiment Construction

[0022] The following examples describe in detail the present invention's DNA molecular genetic marker technology in the Epinmoss microsatellite core sequence of moire grouper. Firstly, the genomic DNA in the blood of the moire grouper was extracted and diluted for later use; then, using the microsatellite core sequence contained in the DNA sequence of the moire grouper, specific primers were designed at both ends of the sequence; PCR amplification is performed on the genomic DNA of individuals in different geographical groups of grouper or moire grouper groups, and denatured polyacrylamide gel electrophoresis detection is performed on the PCR products to determine the genotype of each individual, that is, to obtain moire grouper Genetic polymorphism map of fish.

[0023] 1. Extraction of grouper genomic DNA: Add 100µl of grouper blood into an Eppendorf tube, then add 500µl of cell lysate and 5µl of 20mg / ml proteinase K, shake gently, and leave overnight at 37°C. Then, 600 µl ...

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Abstract

The invention discloses a detection primer and a method of saladfish microsatellite marker Epinmoss. The method comprises the following steps: firstly extracting a genome DNA in a saladfish blood and diluting for later use; designing a specific primer by using a microsatellite core sequence contained in the saladfish DNA sequence; performing PCR amplification on the genome DNA of different saladfish proles or saladfish individuals and detecting the PCR product; analyzing the stripe of the product, and ensuring the genotype of each individual to obtain a genetic polymorphism atlas of the saladfish. The heritable variation atlas of the saladfish genetic marker Epinmoss can be rapidly obtained, the method is simple and the genotype of each individual of the saladfish can be intuitively detected through the result; and the detection primer is applied to the genetical mark and the genealogical identification of different saladfish proles or saladfish individuals and the construction of the genetic map.

Description

technical field [0001] The invention belongs to moire grouper ( Epinephelus moara ) The DNA molecular genetic marker technology relates to a technical method for detecting the microsatellite marker Epimoss of the moire grouper, specifically a detection method for the microsatellite marker Epimoss of the moire grouper. Background technique [0002] Moire Grouper ( Epinephelus moara ) belongs to the order Perciformes, Scorpidae, and the genus Grouper, commonly known as grass spots and oil spots. It is distributed in the western North Pacific Ocean, and it is produced in the East China Sea and South China Sea in China. It has delicious meat, smooth taste, high protein content and rich nutrition, and is favored by consumers. Moire grouper grows fast, has strong adaptability and high economic value, and is suitable for ponds, cages and indoor factory farming. However, the amount of natural resources of moire grouper is small, and it is difficult to meet people's needs. In 201...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 刘云国刘凌霄李瑶瑶李彦伸高永林
Owner 临沂小鲁生物科技有限公司