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A kind of raspberry leaf crude polysaccharide and its preparation method and application

A technology of raspberry leaf and crude polysaccharide, applied in the field of raspberry leaf crude polysaccharide and its preparation, can solve the problem of lack of research on raspberry leaf polysaccharide

Active Publication Date: 2017-09-29
汉方萃取生物科技(海南)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many studies on raspberry fruit polysaccharides, but there are no studies on raspberry leaf polysaccharides.

Method used

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  • A kind of raspberry leaf crude polysaccharide and its preparation method and application
  • A kind of raspberry leaf crude polysaccharide and its preparation method and application
  • A kind of raspberry leaf crude polysaccharide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 prepares raspberry leaf crude polysaccharide (L-Ps) from palm leaf raspberry leaf

[0050](1) Raspberry palmate leaves are crushed and passed through a 20-mesh sieve, weighed 100g, and heated under reflux with distilled water for extraction. The number of times is 3 times; then the extracts are combined, centrifuged at 4000rpm / min for 10min, the supernatant liquid is taken, filtered through filter paper for 3 times, and the filtrate is concentrated to 200mL under reduced pressure at 40°C with a rotary evaporator to obtain a polysaccharide solution;

[0051] (2) D354FD resin was first soaked in distilled water for 12 hours, then soaked in 5% hydrochloric acid solution and 5% sodium hydroxide solution for 0.5 hours respectively, filtered through 200 mesh gauze, and washed with distilled water until neutral. Obtain pretreated D354FD resin;

[0052] (3) Mix the polysaccharide solution prepared in step (1) with 200 mL of pretreated D354FD resin, place in a cons...

Embodiment 2

[0054] Embodiment 2 prepares raspberry leaf crude polysaccharide (L-Ps) from the palm leaf raspberry leaf

[0055] (1) Raspberry palmate leaves are crushed and passed through a 20-mesh sieve, weighed 100g, and then heated and refluxed with distilled water for extraction, wherein the ratio of water to material is 20:1, the reflux extraction temperature is 85°C, the reflux extraction time is 3 hours, and the number of reflux extractions 2 times; then combine the extracts, centrifuge at 3000rpm / min for 8min, take the supernatant liquid, filter it twice with filter paper, and concentrate the filtrate to 200mL under reduced pressure at 50°C with a rotary evaporator to obtain a polysaccharide solution;

[0056] (2) D354FD resin was first soaked in distilled water for 24h, then soaked in 5% hydrochloric acid solution and 5% sodium hydroxide solution for 2h respectively, filtered through 200 mesh gauze, washed with distilled water until neutral, and obtained Pretreated D354FD resin; ...

Embodiment 3

[0059] Embodiment 3 prepares raspberry leaf crude polysaccharide (L-Ps) from the palm leaf raspberry leaf

[0060] (1) Raspberry palmate leaves were crushed and passed through a 20-mesh sieve, weighed 100g, and heated under reflux with distilled water for extraction. The number of times is 4 times, then the extracts are combined, centrifuged at 5000rmp / min for 12min, the supernatant liquid is taken, filtered 4 times with filter paper, and the filtrate is concentrated to 200mL under reduced pressure at 60°C with a rotary evaporator to obtain a polysaccharide solution;

[0061] (2) D354FD resin was first soaked in distilled water for 18h, then soaked in 5% hydrochloric acid solution and 5% sodium hydroxide solution for 3h respectively, filtered through 200 mesh gauze, washed with distilled water until neutral, and obtained Pretreated D354FD resin;

[0062] (3) Mix the polysaccharide solution prepared in (1) with 200 mL of pretreated D354FD resin, place in a constant temperature...

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Abstract

The invention belongs to the field of traditional Chinese medicines, and in particular relates to a raspberry leaf crude polysaccharide, a preparation method and application thereof. The present invention extracts raspberry leaf crude polysaccharide from raspberry leaves through steps such as hot water reflux extraction, decolorization treatment and alcohol precipitation, and the crude polysaccharide can significantly inhibit the release of NO from RAW264.7 macrophages induced by LPS in vitro. Moreover, it can significantly inhibit the expression of TNF-α, iNOS and IL-6 mRNA in RAW264.7 macrophages induced by LPS. In addition, the present invention further purifies the crude polysaccharide of raspberry leaf by ion exchange column chromatography to obtain three components, among which the component with the highest content is the refined polysaccharide of raspberry leaf, which is composed of five monosaccharides Heteropolysaccharide, wherein the molar ratio of sugar residues is: rhamnose:arabinose:xylose:glucose:galactose=2.47:4.75:4.12:1:2.48.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a raspberry leaf crude polysaccharide, a preparation method and application thereof. Background technique [0002] One of the most promising current anti-inflammatory alternatives to classical antibiotic therapy is the use of immunomodulators that enhance host defense responses. A variety of immunomodulators are known, including mammalian proteins, such as: γ-interferon, granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor, and substances isolated and purified from microorganisms. For immune bacterial polysaccharides and synthetic compounds, their adverse reactions and side effects have attracted widespread attention. Most polysaccharides derived from higher plants are non-adverse substances and will not cause major side effects on the body. Therefore, polysaccharides isolated from plants have attracted great attentio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P29/00
Inventor 姜建国张恬恬梁颖婕
Owner 汉方萃取生物科技(海南)股份有限公司
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