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System for Embryo Loading into Cryocarrier, Cryocarrier and Cryopreservation Method

A technology of freezing carriers and embryos, applied in the biological field, can solve the problems of no objective standards and achieve the effect of reducing volume

Active Publication Date: 2019-05-21
张孝东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But for the second point, in actual work, the egg / embryo and the cryoprotectant are transferred to the vitrified carrier through the operation of the experimenter, and the volume of the cryoprotectant around the egg / embryo is completely determined by the operator's proficiency no objective criteria

Method used

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  • System for Embryo Loading into Cryocarrier, Cryocarrier and Cryopreservation Method
  • System for Embryo Loading into Cryocarrier, Cryocarrier and Cryopreservation Method
  • System for Embryo Loading into Cryocarrier, Cryocarrier and Cryopreservation Method

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Embodiment Construction

[0033] The present invention is based on a vitrification method. Specifically, the present invention realizes a system for loading embryos into a freezing carrier, which includes a petri dish and an egg or embryo vitrification carrier. Different from the prior art, the egg or embryo in the present invention Vitrified cryocarriers include:

[0034] The carrier body 1 is integrally connected with the carrier body 1 and drags the tow bar 2 of the carrier body 1, wherein the carrier body 1 is a grid carrier (refer to figure 2 ), for different embryo diameters, the mesh aperture size of the mesh carrier can be changed. For example, in this embodiment, the mesh aperture of the mesh carrier is 80-100um. As the embryo grows, the mesh aperture decreases It can be set larger, so I won’t repeat it here.

[0035] In addition, the grid carrier in this embodiment has an arc in the longitudinal section, and the arc is mainly set by using the siphon principle and the liquid surface tension. Durin...

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Abstract

The invention discloses a system for feeding embryos into a freezing carrier, the freezing carrier and a freezing preservation method. The ovum or embryo vitrification freezing carrier comprises a carrier body and a dragging rod, wherein the dragging rod is integrally connected with the carrier body and is used for dragging the carrier body; the carrier body is a grid bearing body; a longitudinal cross section of the grid bearing body has a radian. As the longitudinal cross section of the grid bearing body of the ovum or embryo vitrification freezing carrier has the radian, after a liquid bridge is formed at the bottoms of the grid bearing body and a petri dish, a liquid with high potential energy and large pressure intensity on the grid bearing body can be continuously pressed into the bottom of the petri dish by virtue of a siphon principle, and the volume of a freezing protecting agent wrapping the periphery of ova / embryos can be reduced when the embryos are fed into the vitrification freezing carrier.

Description

Technical field [0001] The invention relates to the field of biotechnology, and more specifically to a system for loading embryos into a freezing carrier, a freezing carrier and a freezing preservation method. Background technique [0002] Freezing technology has become an indispensable link in human assisted reproductive technology. With the continuous development of freezing technology, slow freezing has gradually been replaced by vitrification. Vitrification technology uses ultra-high cooling speed (>2000℃ / min) to make the eggs / embryos wrapped in high-concentration cryoprotectants quickly form a vitreous state to avoid ice crystals in the cells from damaging the cells, thereby greatly improving the eggs / embryos Survival rate, recovery rate and developmental potential. [0003] Generally, the high-speed cooling process can be achieved through two aspects: one is through the temperature of the cooling medium (through the open carrier quickly put into liquid nitrogen); the othe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 张孝东黄国宁
Owner 张孝东