Method for preparing compound inoculants by decomposing non-water soluble potassium-containing shale with torulaspora globosa

A water-insoluble, compound bacterial agent technology, applied in the direction of bacteria, fungi, etc., can solve the problems of increasing labor and cost, and achieve the effect of reducing production labor and production costs, less investment, and maintaining stability

Inactive Publication Date: 2015-09-02
TONGREN UNIV
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Problems solved by technology

Therefore, when spraying EM liquid, a certain amount of other fertilizers must be applied to supplement the mineral elements needed for plant growth, which undoubtedly increases the labor and cost in production.

Method used

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Embodiment Construction

[0020] All types of media and utensils involved in the following experimental operations were sterilized before inoculation. Sterilization conditions are: autoclave at 120°C and 1 standard atmospheric pressure for 20 minutes,

[0021] The first step: control the temperature of the full-temperature oscillating shaker to 30° C., and the rotation speed to 160 rpm. A strain of Torula spores purchased from the China Agricultural Microorganism Culture Collection and Management Center (No. 20321) was placed in the liquid medium Cultivate the upper medium for 48 hours until the number of bacteria is 10 7 Each / mL, the composition of the liquid medium is: 2% glucose; 0.5% yeast extract; 1% malt extract; 0.2% potassium dihydrogen phosphate.

[0022] Step 2: In a 500mL Erlenmeyer flask, inoculate 2mL of Torula spores liquid into 200mL of ore leaching medium at a volume ratio of 1:100, and add 0.8g of non-water-soluble Potassium-containing minerals, cultured for 15 days to obtain bioleac...

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Abstract

The invention discloses a method for preparing compound inoculants by decomposing non-water soluble potassium-containing shale with torulaspora globosa. The method comprises the following steps: torulaspora globosa (ACCC ID: 20321) is used for decomposing non-water soluble potassium-containing shale in a liquid fermentation medium so as to obtain a leachate containing potassium, phosphorus, sulfur, magnesium, calcium, zinc, copper and other minerals needed for plants; the leachate is mixed with a concentrated EM liquid according to a volume ratio of 2: 1 so as to obtain the compound inoculants which mainly contain mineral element potassium and further contain phosphorus, sulfur, magnesium, calcium, zinc and copper. According to the method, non-water soluble potassium-containing minerals are used as the raw materials to alleviate the situation of lack of potash fertilizer resources in our country and reduce the cost; meanwhile, the method is low in pollution and energy consumption and is expected to have good economic and social benefits.

Description

technical field [0001] The invention relates to a method for preparing a composite microbial agent by using Torula spores to decompose water-insoluble potassium-containing shale, in particular to a method for preparing compound bacteria containing various plants by using Torula spores to decompose water-insoluble potassium-containing shale The method of the compound bacterial agent of the required mineral elements. Background technique [0002] Potassium fertilizer is one of the three commonly used fertilizers that are indispensable in agricultural activities. In recent years. The amount of potassium fertilizer application in my country has been on the rise, but the storage of soluble potassium mineral resources in my country is low, which cannot meet the needs of growing agriculture, and the contradiction between supply and demand is prominent. Therefore, how to increase the supply of potassium fertilizer in our country has become an urgent problem to be solved. In my co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14
CPCC12N1/20C12N1/14C12N1/16
Inventor 舒华姚元勇唐帮成谢子楠吴思展
Owner TONGREN UNIV
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