A kind of antioxidant bone collagen polypeptide and preparation method thereof
A collagen peptide and antioxidant technology, which is applied in the preparation methods of peptides, chemical instruments and methods, and food ingredients as antioxidants, etc., can solve the problems of single product source and immunogenicity.
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[0020] The preparation method provided by the invention is as follows:
[0021] (1) Extraction of tuna collagen hydrolyzate
[0022] Take 10 g of tuna bones, wash them, soak them in 1.25 mol / L hydrochloric acid for 12 hours, and change the acid every 1.5 hours to remove inorganic salts. Then the tuna bone was cleaned to a pH of 7.0, and then the enzymolysis conditions were pH 8, temperature 37°C, and enzymolysis time 2 hours, according to the conditions that the volume concentration of trypsin was 0.5%, and the volume concentration of chymotrypsin was 0.1%. trypsin and chymotrypsin, the supernatant was collected, filtered, concentrated and freeze-dried to obtain tuna collagen hydrolyzate.
[0023] (2) Separation and purification of enzymolysis products
[0024] The enzymatic hydrolysis product was separated by DEAE-52 cellulose anion collagen chromatography, and the unadsorbed components were washed away after loading the sample, and then eluted with pH 7.2 phosphate buffer ...
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