Functionalized nano-graphene oxide-loaded insulin derivative material and its preparation and application in the preparation of drugs for the treatment of diabetes

A technology of insulin derivatives and nano-graphite oxide, which can be used in drug combination, metabolic diseases, pharmaceutical formulations, etc., can solve problems such as undiscovered, and achieve the effects of simple steps, alleviating physical and mental pain, and direct preservation and use.

Inactive Publication Date: 2017-11-07
JINAN UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no reports have been found about the use of aminated polyethylene glycol-functionalized nano-graphene oxide-loaded insulin derivatives in inhibiting diabetes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Functionalized nano-graphene oxide-loaded insulin derivative material and its preparation and application in the preparation of drugs for the treatment of diabetes
  • Functionalized nano-graphene oxide-loaded insulin derivative material and its preparation and application in the preparation of drugs for the treatment of diabetes
  • Functionalized nano-graphene oxide-loaded insulin derivative material and its preparation and application in the preparation of drugs for the treatment of diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Preparation of nano-graphene oxide

[0038]Adopt conventional Hummers method to prepare graphene oxide, weigh 3g natural graphite powder and 18g potassium permanganate and mix evenly, add concentrated sulfuric acid 360mL and concentrated phosphoric acid 40mL mixed acid (volume ratio 9:1), stir and heat to keep the heating temperature of reactant Reaction between 35 ~ 40 ℃ for 1h. Then the reactant was rapidly heated to 50°C and the reaction was continued for 12h. After the reaction was completed, it was naturally cooled to room temperature. Quickly add 100mL of ice water into the above system, stir for 5min and then dropwise add 4mL of 30% H 2 o 2 The solution turned from black to khaki. Let it stand for 2 hours, pass through a metal standard test screen (W.S.Tyler, 300 μm), centrifuge the filtrate at 15,000 rpm for 30 minutes, discard the supernatant, and wash the lower layer of solid matter twice with 200 mL of water, 200 mL of 30% HCl and 200 mL of ethanol ,...

Embodiment 2

[0044] (1) the preparation of nanometer graphene oxide is the same as embodiment 1;

[0045] (2) the preparation of aminated polyethylene glycol functionalized nano-graphene oxide is the same as in Example 1;

[0046] (3) Preparation of Aminated Polyethylene Glycol Functionalized Nano Graphene Oxide Loaded Insulin Derivative Materials

[0047] Weigh 10 mg of nGO@PEG prepared in step (2) and dissolve in 10 mL of PBS to prepare a 1 mg / mL solution. Dissolve 2 mg of insulin-derived short peptide EALYLV (relative molecular weight: 707) with 1 mL of PBS, and divide 1 mL of the solution into 5 parts, each with a concentration of 2.83×10 -3 mol / L as a stock solution. Ultrasonic disperse 10mL of nGO@PEG solution at 570W for 10min, then add 20mg EDC·HCl and 40mg NHS to maintain the pH value of the solution at about 7, then add 400μM EALYLV stock solution with a rubber dropper, and stir at 25°C Reaction 2h. After the reaction was completed, centrifuge at 6000 rpm for 30 min, discard ...

Embodiment 3

[0049] (1) the preparation of nanometer graphene oxide is the same as embodiment 1;

[0050] (2) the preparation of aminated polyethylene glycol functionalized nano-graphene oxide is the same as in Example 1;

[0051] (3) Preparation of Aminated Polyethylene Glycol Functionalized Nano Graphene Oxide Loaded Insulin Derivative Materials

[0052] Weigh 20 mg of nGO@PEG prepared in step (2) and dissolve in 10 mL of PBS to prepare a 1 mg / mL solution. Dissolve 2 mg of insulin-derived short peptide EALYLV (relative molecular weight: 707) with 1 mL of PBS, and divide 1 mL of the solution into 5 parts, each with a concentration of 1.83×10 -3 mol / L as a stock solution. Ultrasonic disperse 10mL of nGO@PEG solution at 300W for 10min, then add 40mg EDC·HCl and 80mg NHS to maintain the pH value of the solution at about 7, then add 400μM EALYLV stock solution with a rubber dropper, and stir at 25°C Reaction 2h. After the reaction was completed, centrifuge at 6000 rpm for 30 min, discard ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical field of preparation of medicine for treating diabetes, and discloses an amino polyethylene glycol functionalized nano graphene oxide supported insulin derivative material, a preparation method thereof and application thereof in preparation of the medicine for treating diabetes mellitus type 2. The material is prepared through the method including the following steps: adding amino polyethylene glycol into a graphene oxide solution, then adding 1-ethyl-(3-dimethyl aminopropyl) carbonyl diimine hydrochloride and N-hydroxysuccinimide, adjusting the pH, stirring while reacting, and separating in a centrifugal manner, so as to obtain amino polyethylene glycol modified nano graphene oxide; dispersing amino polyethylene glycol modified nano graphene oxide into a solution, adding 1-ethyl-(3-dimethyl aminopropyl) carbonyl diimine hydrochloride and N-hydroxysuccinimide, dropwise adding an insulin derivative, and stirring while reacting, so as to obtain the amino polyethylene glycol functionalized nano graphene oxide supported insulin derivative material. The material provided by the invention can significantly inhibit human pancreas islet amyloid protein aggregation.

Description

technical field [0001] The invention belongs to the technical field of preparing drugs for treating diabetes, and in particular relates to an aminated polyethylene glycol functionalized nano-graphene oxide-loaded insulin derivative material (nGO@PEG@EALYLV, nGO@PEG@E for short) and its preparation method and Application in preparation of medicine for treating type 2 diabetes. Background technique [0002] Diabetes mellitus (DM) is an endocrine and metabolic disease characterized by elevated blood sugar, which is caused by multiple pathogenic factors such as genetic factors, environmental factors, and behavior. Diabetes has become a worldwide public health problem in the 21st century. Insulin is currently an effective drug for the treatment of diabetes, but it can only temporarily alleviate the patient's blood sugar elevation through injection. Long-term injection will not only have a serious impact on the patient's body and mind, but will also bring a heavy economic burden....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/52A61K38/28A61K47/10A61P3/10
Inventor 刘杰周贤波俆桃远
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap