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Application of biomembrane or closed structure or cellular compartment with biomembrane properties as cosmetic or cosmetic carrier

A cosmetic carrier and cell compartment technology, applied in the fields of biomedicine and polymer materials, to achieve the effect of improving solubility

Inactive Publication Date: 2015-10-28
HANGZHOU UMOTOR BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The technical improvements described by this patented technology include improving its ability to dissolves poorly absorbed or immobilized chemicals (such as sunscreen agents) on their surface for better penetrating them throughout our body). This results from an increase in hydrolipid membranes around these molecules due to the presence of certain lipids called lamellar structures at both ends of each layer within it. These structural features allow some compounds like camouflage dyes to stay inside while still being able to get deeper down into tissues without getting stuck up with other materials they might cause damage during treatment procedures such as surgery or chemotherapy treatments.

Problems solved by technology

Technologies described involve creation of artificial films made up entirely of lipides and protein hydrolyzites, but there exist technical problem addressed in the patented text relations due to lack of stable and reliable carriers containing fluorescent compounds inside. Additionally, current methods require adding chemical agents during manufacturing processes may affect the quality and performance of the resulting film products.

Method used

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  • Application of biomembrane or closed structure or cellular compartment with biomembrane properties as cosmetic or cosmetic carrier
  • Application of biomembrane or closed structure or cellular compartment with biomembrane properties as cosmetic or cosmetic carrier
  • Application of biomembrane or closed structure or cellular compartment with biomembrane properties as cosmetic or cosmetic carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Extraction and purification of biofilm

[0055] The desired biofilm was extracted and purified by density gradient centrifugation. The specific process is as follows:

[0056] (1) New Zealand white rabbits that had been fasted for 18-24 hours were sacrificed to take the liver, and after removing the large blood vessels, the liver was cut into 2mm 3 The left and right small pieces were washed with normal saline until the tissue pieces turned white;

[0057] (2) Preparation of homogenate: 1mmol / L CaCl 2 , 50mmol / L HEPES (pH 7.4), 1mmol / L PMSF, 2μg / mL Aprotinin, 2μg / mL Antipain;

[0058] (3) Add liver tissue and homogenate to 8 times the volume of homogenate according to the mass:volume ratio, and homogenize with an electric homogenizer at 15,000 rpm in an ice-bath environment until the tissue is liquefied;

[0059] (4) Filter the homogenate through four layers of gauze, centrifuge the filtrate at 1,000×g, 10min, 4°C, and collect the precipitate;

[0060] ...

Embodiment 2

[0062] Example 2 Extraction and purification of biofilm

[0063] The desired biofilm was obtained by extracting and purifying by two-phase partition method. The specific process is as follows:

[0064] (1) Select plump and consistent normal corn seeds, soak them in 1% NaClO for 10 minutes, rinse them, and germinate them at 25°C in constant temperature and darkness for 72 hours;

[0065] (2) Take corn epicotyl, add 2 times the volume of extraction buffer (5mmol / L EDTA, 25mmol / L Tris, 0.25mmol / L sucrose, 1mmol / L MgSO 4 , 0.2% (W / V) BSA, 0.5% (W / V) PVP-10, 10% (W / V) glycerol, 15mmol / L β-mercaptoethanol, 1mmol / L PMSF, 1mmol / L DTT), ice bath grinding to liquefaction;

[0066] (3) Filter the grinding solution through four layers of gauze, centrifuge the filtrate at 12,000×g, 15min, 4°C, and take the supernatant;

[0067] (4) Centrifuge the supernatant at 80,000×g for 30 minutes at 4°C to collect the precipitate;

[0068] (5) Precipitate with suspension (25mmol / L Tris, 0.25mmo...

Embodiment 3

[0070] Example 3 Extraction and purification of biofilm

[0071] The desired biofilm was obtained by extracting and purifying by differential centrifugation. The specific process is as follows:

[0072] (1) Antarctic ice algae ( Chlamydomonas subcaudata ) is isolated and purified from Antarctic sea ice;

[0073] (2) Inoculate Antarctic ice algae into the medium at a ratio of 1:100 (21.2g NaCl, 3.6g NaSO per 10L medium 4 , 0.6g KCl, 0.3g NaHCO 3 , 0.1g KBr, 0.1g H 3 BO 3 , 0.1g NaF, 9.6g MgCl 2 ·6H 2 O, 1.0 g CaCl 2 , 0.1g SrCl 2 ·6H 2 O), cultured in a controlled light incubator. Cultivate for 14 days at -4°C, with a light intensity of 1300~1900lx, a light cycle of 12 light / 12 dark, and shaking 4~5 times a day;

[0074] (3) Centrifuge the ice algae culture solution at 4,000rpm, 20min, and 4°C to collect the ice algae sediment, and rinse it twice quickly with pre-cooled distilled water to remove extracellular viscous, surface salt and impurities in the culture so...

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Abstract

The invention belongs to the field of biomedical and macromolecular materials, and particularly relates to application of a biomembrane or a closed structure or cellular compartment with biomembrane properties as a cosmetic or cosmetic carrier. The biomembrane or the closed structure or cellular compartment with the biomembrane properties is obtained from natural sources or obtained by self-assembly, and biological sources include animals, plants and microorganisms. The obtained biomembrane is integrated or fragmented and is of a lipid bilayer structure in form; the constituents of the biomembrane mainly include lipid and protein, and besides, a few carbohydrates are combined to the lipid or the protein through covalent bonds. Being of the lipid bilayer structure, the biomembrane has the moisturizing, skin whitening and anti-aging effects when applied to the field of cosmetics; meanwhile, serving as the carrier of functional constituents, the biomembrane has the effects of improving stability, solubility and long effectiveness and promoting absorption.

Description

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Claims

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Application Information

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Owner HANGZHOU UMOTOR BIOTECH CO LTD
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