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A method for establishing an animal model of pancreatic cancer with inflammatory basis

An animal model and a technology for establishing a method, applied in the field of establishing an animal model of pancreatic cancer

Inactive Publication Date: 2018-05-01
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it has not been confirmed whether DBTC can induce pancreatitis in nude mice

Method used

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  • A method for establishing an animal model of pancreatic cancer with inflammatory basis
  • A method for establishing an animal model of pancreatic cancer with inflammatory basis

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Embodiment 1

[0087] A method for establishing an animal model of pancreatic cancer with an inflammatory basis, the steps are as follows:

[0088] The first step, the construction of pLVX-luciferase-IRES-ZsGreen1 plasmid:

[0089] 1) Prepare pLVX-IRES-ZsGreen1 digestion product and pGL3-Basic digestion product through the following reaction system and conditions,

[0090] reaction system:

[0091] DNA (pLVX-IRES-ZsGreen1, pGL3-Basic plasmids), 1 μg

[0092] 10×NEbuffer4 (+BSA), 3 μL

[0093] Endonuclease XhoI, 1 unit

[0094] Endonuclease XbaI, 1 unit

[0095] Add sterilized ultrapure water to volume 30 μL

[0096] Reaction conditions: 37°C water bath for 2h.

[0097] 2) Perform agarose gel electrophoresis on the pGL3-Basic digestion product, cut the gel and recover the gel block containing the 1700bp DNA fragment, and purify it with the AxyPrep DNA Gel Recovery Kit to obtain the luciferase DNA fragment, which uses The specific steps for purification with the AxyPrep DNA Gel Extracti...

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Abstract

The present invention provides a method for establishing an animal model of pancreatic cancer with an inflammatory basis, the steps are as follows: the first step, the construction of pLVX-luciferase-IRES-ZsGreen1 plasmid; the second step, the establishment of lentivirus-mediated luciferase overexpression system ; the third step, the establishment of pancreatic cancer cell lines stably expressing luciferase; the fourth step, the establishment of pancreatic orthotopic xenograft tumor model in nude mice; the fifth step, the establishment of inflammation model; the sixth step, pancreatic cancer with inflammatory basis Establishment of animal model: continue to feed nude mice for 30 days, and establish a pancreatic cancer model with an inflammation basis in the nude mice. The animal model of pancreatic cancer established through the above steps can be used to evaluate the size of the tumor after the use of Luciferase, which catalyzes the luminescence of the substrate, is detected by the corresponding equipment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing an animal model of pancreatic cancer with an inflammatory basis. Background technique [0002] Epidemiological and clinical studies have confirmed that about 25% of adult tumors are caused by chronic inflammation. For example, chronic viral or bacterial infections of the stomach, liver, and cervix, as well as chronic exposure of the lungs to tobacco smoke or other environmental compounds, significantly contribute to chronic tissue damage and subsequent neoplasia in these organs [1]. Chronic inflammation is closely related to the occurrence and development of pancreatic cancer [2]. The incidence of pancreatic cancer in the general population is approximately 0.007% [3], increasing to 4% in patients with chronic pancreatitis and as high as 40% in patients with hereditary pancreatitis [4]. The use of anti-inflammatory agents has been associated wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/867C12N5/10A01K67/027
Inventor 李伟吴梦瑶陶敏龚斐然
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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