A kind of tissue culture method of Polygonatum yunnanensis seedling in one step
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A technology of Rhizoma Polygonatum and seeds, which is applied in the field of tissue culture of Rhizoma Polygonatum seedlings in one step, can solve the problems of high production cost, high technical difficulty, and long time period, and achieve the effects of low cost, shortened germination period, and well-developed root system
Active Publication Date: 2017-03-08
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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[0009] The purpose of the present invention is to overcome the defects of complex procedures, high technical difficulty, long time period and high production cost in the prior art, and provide a new method for tissue culture of Polygonatum yunnanensis seedlings in one step
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Embodiment 1
[0032] This example was implemented in the laboratory of the Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming City, Yunnan Province.
[0033] (1) Selection of the fruits of Polygonatum yunnanensis
[0034] In October, 80% of ripe, fresh, disease-free Rhizoma Polygonati fruit was selected and stored in a refrigerator at 4°C for 7 days.
[0035] (2) Treatment of Polygonatum yunnanensis seeds
[0036] Peel off the skin of the selected Dianhuangjing fruits, take out the seeds and put them in a beaker, sterilize with 75% alcohol by volume for 1 min, rinse twice with sterile water, transfer to a sterile workbench, and use a mass fraction of 0.1% HgCl 2 After soaking in the solution for 14 minutes, rinse with sterile water 3 times;
[0037] (3) One-step seedling cultivation of Yunnan Polygonatum seeds
[0038] The seeds of Polygonatum yunnanensis treated in step (2) were inoculated into a culture flask containing MS+BA 0.5mg / L medium at a culture temperature of 27...
Embodiment 2
[0042] Embodiment 2 is the same as Embodiment 1 except for the following parameters, and will not be repeated.
[0043] (2) Treatment of Polygonatum yunnanensis seeds
[0044] The taken out seeds are disinfected with 75% alcohol by volume for 1 min and then rinsed 3 times with sterile water; transferred to a sterile workbench, with a mass fraction of 0.1% HgCl 2 Soak the solution for 15 minutes and rinse 4 times with sterile water;
[0045] (3) One-step seedling cultivation of Yunnan Polygonatum seeds
[0046] Inoculate the Yunnan Polygonatum seeds treated in step (2) in a culture flask containing MS+BA 0.5mg / L medium. The density of the Yunnan Polygonatum seeds in the culture flask is 10 or 15 seeds / bottle, the seed density is too dense It is not conducive to the growth of plants, and it will waste the medium if it is too thin. The diameter of the culture flask is 7cm, the height is 11cm, the culture temperature is 23℃, the light time is 14h / day, and the light intensity is 150lx. Wh...
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Abstract
The invention discloses a tissue culture method for culturing Polygonatum kingianum seeds into seedlings in one step. The method comprises steps as follows: selecting Polygonatum kingianum fruits, treating Polygonatum kingianum seeds, culturing the Polygonatum kingianum seeds into the seedlings in one step and performing seedling exercise and transplantation. Medium-well mature fruits are selected and placed in a refrigerator at the temperature of 4 DEG C for 7 days; a culture bottle containing an MS+BA 0.5 mg / L culture medium is inoculated with the treated seeds, the seeds are cultured under the conditions that the temperature is 25 DEG C plus / minus 2 DEG C, the illumination time is 12-14 h / day and the illumination intensity is 150 lx until the Polygonatum kingianum seeds send forth two true leaves, and the seeds are continuously cultured under the conditions that the culture bottle is placed at the culture temperature of 25 DEG C plus / minus 2 DEG C, the illumination time is 12 h / day and the illumination intensity is 1500 lx until rooting. With the adoption of the method, only 90-99 days are taken from inoculation for the seeds to one-step culture of the seedlings, the germination period is shortened to 1 month from two winters, the propagation cycle is greatly shortened, the seed germination rate is in a range of 90%-95%, and the excised seedling survival rate is 100%.
Description
Technical field [0001] The invention belongs to the technical field of planting traditional Chinese medicinal materials, and particularly relates to a tissue culture method for seedling formation of Polygonatum yunnanensis seeds in one step. Background technique [0002] Polygonatum kingianum Coll.et Hemsl. is a herb of the genus Polygonatum kingianum Coll.et Hemsl. As a commonly used Chinese medicine, Polygonatum has a history of more than 2,000 years of medication in my country. Its nature and taste are sweet and flat, and it enters the lung, spleen and kidney channels. Efficacy: Nourish the heart and lungs, strengthen the muscles and bones. It can cure deficiency, cold and heat, tuberculosis, hemoptysis, lack of food after illness, weak muscles and bones, rheumatic pain, and rheumatoid ringworm. In recent years, pharmacological studies have shown that in addition to the above-mentioned effects, Polygonatum has antibacterial effects, especially for Mycobacterium tuberculosis ...
Claims
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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 季鹏章张智慧王家金石亚娜刘大会朱新焰王丽马聪吉
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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