Method and device for collecting signals by photosensitive chip and method and device for tracking cells

Abstract

The invention discloses a method and device for collecting signals by a photosensitive chip and a method and device for tracking cells, and relates to the technical field of signal collection. On the photosensitive chip, the photosensitive chip attached with the film carrying the light signal to be collected is placed in a dark room, the light signal is collected in the dark room, and the collected light signal is processed and output. The method and device for collecting a signal from a photosensitive chip provided by the present invention complete the signal acquisition by contacting the photosensitive chip, convert the optical signal into a digital signal, and complete quantitative analysis well.

Description

technical field [0001] The invention relates to the technical field of information collection, in particular to a method and device for collecting signals by a photosensitive chip and a method and device for tracking cells. Background technique [0002] Western blotting is a hybridization technique that combines high-resolution gel electrophoresis and immunochemical analysis techniques. Western blotting has the advantages of large analysis capacity, high sensitivity, and strong specificity. It is the most commonly used method for detecting protein characteristics, expression, and distribution, such as qualitative and quantitative detection of tissue antigens, mass determination of polypeptide molecules, and detection of viruses. Antibody or antigen detection, etc. [0003] Existing devices and methods for collecting Western blotting signals: [0004] Method 1: Put the photosensitive film and the NC film close together, after exposing for a certain period of time, develop a...

AI Technical Summary

Problems solved by technology

There are the following disadvantages: 1. It takes up a lot of space: special darkrooms (rooms), sinks, and sewer pipes, etc. are required

2. High cost: Not only do you need to buy film processors and cassettes, but you also need a lot of consumables such as photosensitive film, developer, and fixer

Washing the film also causes waste of water resources

3. Pollution of the environment: developing film requires a lot of developer and fixer

4. Unstable image quality: In the darkroom, researchers cannot monitor the exposure level in real time, and often get a better image after several attempts

Time-consuming

5. Time-consuming: Nowadays, digitalization is widely used for data storage, transmission and publication. Therefore, film images have to be scanned and converted into digital images

6. Quantitative inaccuracy: Most of the time, the image that researchers judge to be better with the naked eye is actually oversaturated in grayscale

Disadvantage: loses the advantages of film acquisition, that is, the sensitivity is severely reduced

Disadvantages: Since the optical signal is collected by linear scanning, the entire image cannot be collected at the same time, so there is a time difference between scanning in different areas

And because the light source is continuously attenuated with time, the signal strength collected at different time points will not be comparable

Makes many controlled trials incomparable

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