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Petroleum degrading bacterium as well as preparation method and application thereof

A technology for oil degrading bacteria and oil pollution, which is applied to the restoration of contaminated soil fixed on/in organic carriers, can solve the problems of affecting restoration efficiency, limited storage time, and different effects, and achieves environmental adaptability Strong, easy to transport and store, maintain activity

Active Publication Date: 2015-11-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The degradation process of microorganisms to oil pollution has different effects under different environmental conditions, which affects the repair efficiency; and the bacterial liquid used in practical applications is fermented with rich nutrients, which has limited storage time and inconvenient transportation. question

Method used

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  • Petroleum degrading bacterium as well as preparation method and application thereof
  • Petroleum degrading bacterium as well as preparation method and application thereof
  • Petroleum degrading bacterium as well as preparation method and application thereof

Examples

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preparation example Construction

[0024] A kind of preparation method of petroleum degrading bacterial agent provided by the invention, concrete steps are as follows:

[0025] 1) Dissolve polyvinyl alcohol completely at 80-100°C, and mix it with sodium alginate solution in proportion to obtain a mixed solution of sodium alginate and polyvinyl alcohol;

[0026] 2) Add activated carbon to the mixed solution of sodium alginate and polyvinyl alcohol and mix until the concentration of activated carbon is 5g / L;

[0027] 3) After cooling the solution obtained in step 2) to 40°C, mix it with the oil-degrading bacteria solution at a volume ratio of 10:1;

[0028] 4) Under stirring conditions at 0~4°C, use a syringe to drop the solution obtained in step 3) into a calcium chloride saturated boric acid solution with a concentration of 30% to form a ball;

[0029] 5) Put the solution obtained in step 4) in a refrigerator at 4°C for 26 hours, then take out the microspheres in the solution and wash them with sterilized 0.9%...

Embodiment 1

[0038] A kind of preparation method of petroleum degrading bacterial agent in the present embodiment, concrete steps are as follows:

[0039] 1) Dissolve polyvinyl alcohol completely at 80~100°C, mix it with sodium alginate solution in proportion to obtain a mixed solution of sodium alginate and polyvinyl alcohol; mix sodium alginate (SA) with polyvinyl alcohol (PVA) The mass percentage of sodium alginate in the solution is 1%, the mass percentage of polyvinyl alcohol is 8%, SA:PVA=1:8;

[0040] 2) Add activated carbon to the mixed solution of sodium alginate and polyvinyl alcohol and mix until the concentration of activated carbon is 5g / L;

[0041] 3) After cooling the solution obtained in step 2) to 40°C, mix it with the petroleum-degrading bacterial solution at a volume ratio of 10:1; the petroleum-degrading bacterial solution contains Chryseobacterium, Caulobacter, Achromobacter and Burkholderia Bacterial solution, the mass ratio of the four strains is 36:22:18:5, the OD ...

Embodiment 2

[0051] In this example, the mass percentage of sodium alginate (SA) and polyvinyl alcohol (PVA) in the mixed solution of sodium alginate (SA) and polyvinyl alcohol (PVA) in step 1) is 2%, the mass percentage of polyvinyl alcohol is 8%, SA:PVA=2 : 8; all the other steps are identical with embodiment 1, prepare bacterial agent.

[0052] The mass percentage of petroleum in the petroleum-contaminated soil treated with the bacterial agent was 32%. In this example, the degradation rate of the oil-contaminated soil by the petroleum hydrocarbon-degrading bacterial agent was 13%, and the oil content in the soil changed as figure 2 shown.

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Abstract

The invention discloses a petroleum degrading bacterium as well as a preparation method and an application thereof. The preparation method comprises the following steps: uniformly mixing polyving akohol and sodium alginate solution at a certain ratio, adding with activated carbon, uniformly mixing, cooling, and adding petroleum degrading bacterium solution into the cooled mixture according to the ratio of the cooled mixture to petroleum degrading bacterium solution being 10 to 1, wherein the petroleum degrading bacterium solution contains flavobacterium indologenes, caulobacter, achromobacter and burkholderia cenocepacia at the weight ratio of 36 to 22, to 18 to 5; dropwise adding the mixed solution into calcium chloride saturated boric acid solution through an injection syringe for balling; and standing, taking out microspheres in the solution, washing by adopting sterilized 0.9% NaCl solution, and thus obtaining the solid microspherical petroleum degrading bacterium. The petroleum degrading bacterium can be used for restoring petroleum polluted soil, the solid petroleum degrading bacterium is convenient to transport and store; and technical support is provided for the practical application of restoring the petroleum polluted soil by microorganisms.

Description

technical field [0001] The invention relates to the preparation of a mixed bacterial agent for repairing petroleum-contaminated soil, in particular to a petroleum-degrading bacterial agent and its preparation method and application. Background technique [0002] Petroleum is one of the most important energy sources in modern society, known as "the blood of industry" and "black gold". Petroleum is widely used in industrial fields such as the preparation of natural gas, gasoline, kerosene, volatile oil, lubricating oil, paraffin and asphalt. During the processing, use and transportation of crude oil, it is easy to cause spills and leakage accidents, causing oil to enter water bodies, soil and other environments to cause oil pollution. Petroleum is a complex mixture composed of various hydrocarbons and a small amount of non-hydrocarbon compounds. It is difficult to degrade in the environment. The polycyclic aromatic hydrocarbons in its components are a kind of persistent organ...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/08C12N11/04B09C1/10
Inventor 赵和平孟帆钟亮温丽莲张吟
Owner ZHEJIANG UNIV
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