A method for preparing biological composite trachea patch and its application

A biological and tracheal technology, applied in the field of medical devices, can solve the problems of inability to meet individual needs, complex production process, low cell survival rate, etc. Avoid the effects of rejection

Active Publication Date: 2018-01-02
SHANGHAI PULMONARY HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the 3D printed tracheal patches obtained by current research have disadvantages such as poor biocompatibility, low cell survival rate, complicated manufacturing process, poor product plasticity, and inability to meet individual needs.

Method used

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  • A method for preparing biological composite trachea patch and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Using a bronchoscope, aseptically brush the autologous bronchial epithelial cells of the patient undergoing surgery, culture and expand them in vitro for later use; then use surgery to extract the patient’s auricular cartilage, digest and disperse the chondrocytes, and culture and expand them in vitro for later use.

[0050] Preparation of soft materials: 10% collagen and 5% hyaluronic acid are mixed with deionized water according to the mass-volume ratio, and filtered through a bacteria filter to sterilize. After the chitosan is fumigated with ethanol, it is irradiated under an ultraviolet lamp for 24 hours to completely sterilize it, and is dispersed in the culture solution at a mass volume ratio of 8%. Mix the above two solutions 1:1 to make a soft material mixture for resuspending cells.

[0051] The two types of cells amplified in vitro were digested, resuspended, counted, and centrifuged, the supernatant was discarded, and resuspended in the aforementioned mixture...

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Abstract

The present invention provides a method for preparing a biological composite tracheal patch in combination with 3D printing technology. By precisely regulating the spatial distribution of various cells, improving the patch material formula, and improving the internal structure design, a safe and feasible tracheal patch can be provided clinically. product. The biological material formula of the present invention has good biocompatibility and is used for printing trachea patches, avoiding the dependence on the donor trachea; the material used in the present invention has both flexibility and certain mechanical strength, and the internal structure design of the patch is also improved. Improvement, so that it can be bent and trimmed according to clinical needs; the seed cells expanded from the recipient's own airway epithelial cells and chondrocytes are printed on the patch, which avoids the patient's rejection of the cells; the cell space The distribution is precise and controllable during printing; the implanted patch can be degraded in the body, which reduces the complications caused by foreign matter residues in the body and avoids the risk of secondary surgical removal.

Description

technical field [0001] The invention relates to the field of medical devices, in particular to a method for preparing a biological composite tracheal patch, the prepared tracheal patch and its application. Background technique [0002] Due to various etiologies such as congenital malformation of the trachea, tumor resection, trauma, etc., part of the tracheal wall is often lost. If the trachea is directly sutured longitudinally, it may cause iatrogenic airway stenosis, causing a series of complications including recurrent fatal pneumonia. At this time, cutting the trachea and then anastomosis is the main method to solve this problem. However, after long-segment tracheectomy (more than 50% of the total length in adults and more than 30% in children), the operation cannot be performed because the distance between the two broken ends is too far to cause excessive tension at the anastomotic stoma. Therefore, it is of great significance to find a good patch material to repair th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/26A61L27/20A61L27/24A61L27/18A61L27/58A61L27/50B33Y70/00A61F2/04
Inventor 李牧张洪波陈昶殷瑞雪郑卉邢天龙王龙
Owner SHANGHAI PULMONARY HOSPITAL
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