Preparation method of sCVPs (selenic acid esterified catathelasma ventricosum polysaccharides)

A technology of pine bud mushroom and selenate, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, metabolic diseases, etc., can solve the difficulty of controlling cultivation conditions, loss of selenium-rich polysaccharide yield, and uneven product quality and other issues, to achieve the effect of low price, outstanding anti-diabetic activity, and convenient collection

Inactive Publication Date: 2015-12-02
SICHUAN AGRI UNIV
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Problems solved by technology

At this stage, the methods for selenization modification of polysaccharides are mainly chemical and biological methods. Biological methods have the disadvantages of uneven product quality, poor repeatability, and difficult control of culture conditions; chemical methods often use losses in order to obtain higher selenium content. The yield of selenium-enriched polysaccharides is at the expense, and th

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  • Preparation method of sCVPs (selenic acid esterified catathelasma ventricosum polysaccharides)
  • Preparation method of sCVPs (selenic acid esterified catathelasma ventricosum polysaccharides)
  • Preparation method of sCVPs (selenic acid esterified catathelasma ventricosum polysaccharides)

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[0013] The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0014] The invention provides a preparation method of polysaccharide of selenized pine mushroom, which is characterized in that: (1) taking fresh pine mushroom for pretreatment; cleaning: using a plastic scraper to clean the fresh pine mushroom Remove the mud stains on the surface, do not wash with water, otherwise the polysaccharides on the surface will be lost; cut into pieces: cut the cleaned fresh pine mushrooms into pieces, each piece is moderate and uniform in size; freeze-drying: cut the pine mushrooms into pieces The mushroo...

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Abstract

The invention discloses a preparation method of sCVPs (selenic acid esterified catathelasma ventricosum polysaccharides). A method for preparing CVPs comprises the following steps: catathelasma ventricosum is freeze-dried and ground into powder, the powder is subjected to water extraction and alcohol precipitation with ethanol and subjected to protein removal, small-molecular-weight impurity removal and the like, and the CVPs are obtained. The method for preparing the sCVPs comprises the following steps: 500 mg of CVPs are placed in a three-mouth flask, an HNO3 aqueous solution is added, the mixture is heated and stirred, the CVPs are totally dissolved, and Na2SeO3 and BaCl2 are added respectively when water bath is heated to 70 DEG C, and the mixture has a reaction for 10 h; after the reaction, the product is cooled rapidly to the room temperature, pH is regulated to 6 by anhydrous Na2CO3, then anhydrous Na2SO4 is added to precipitate excess BaCl2, centrifugation, precipitate removal and dialysis are performed, a small quantity of a dialysate is taken and added with vitamin C for detection, dialysis is stopped when red disappears, and powder obtained after freeze-drying is the sCVPs.

Description

technical field [0001] The invention relates to the technical field of preparation of polysaccharides, in particular to a preparation method of selenate-esterified pine mushroom polysaccharides (sCVPs). Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. Hyperglycemia is caused by defective insulin secretion or impaired biological action, or both. The long-term high blood sugar in diabetes leads to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. According to data released by the World Health Organization in 2014, the number of people with diabetes worldwide has reached 347 million. [0003] Edible fungi with medicinal value are considered as one of the very important therapeutic drugs in traditional folk medicine, and are widely used. Studies have shown that the treatment of diseases by edible fungi is mainly caused by the biologically active substances i...

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P3/10
Inventor 刘韫滔曾思琪唐倩倩张鹏李鑫张清李诚刘耀文杨勇陈代文
Owner SICHUAN AGRI UNIV
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