A method for improving the sclerotinization phenomenon of Aspergillus oryzae strains

A technology of Aspergillus oryzae and Aspergillus oryzae, which is applied in the field of improving the sclerotinization phenomenon of Aspergillus oryzae strains, can solve the problems of gene variation, the decline of strain growth ability, and the deterioration of the ability to absorb and utilize nutrients, and achieves less investment and better effect. , Improve the effect of sclerotia

Inactive Publication Date: 2018-05-01
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

[0004] At present, the specific reason for the formation of sclerotia on the slope is not clear, but it is estimated to be related to the decline of the growth ability of the strain, the degradation of the ability to absorb and utilize nutrients, and genetic variation

Method used

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  • A method for improving the sclerotinization phenomenon of Aspergillus oryzae strains
  • A method for improving the sclerotinization phenomenon of Aspergillus oryzae strains
  • A method for improving the sclerotinization phenomenon of Aspergillus oryzae strains

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Embodiment 1

[0029] The Aspergillus oryzae bacterial strain adopted in the present embodiment is:

[0030] Aspergillus oryzae strain A: Sclerotinized fructosyltransferase producing strain Aspergillus oryzae scut209 (Aspergillusoryzae scut209), such as figure 1 shown;

[0031] Aspergillus oryzae strain B: a normal fructosyltransferase production strain preserved in our laboratory. Aspergillus oryzae strain A is a strain of Aspergillus oryzae that has sclerotized after several generations of inoculation. The Aspergillus oryzae strain B is preserved in The Chinese Type Culture Collection Center in Wuhan, China, the collection number is CCTCC NO: M2012106; and it has been disclosed in the authorized invention patent entitled "A strain producing fructosyltransferase and its preparation method", the patent authorization number is: ZL201210374664 .5.

[0032] The formula of mold conventional preservation slant culture medium adopted in this example is: 3.0wt% white granulated sugar, 2.0wt% soyb...

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Abstract

The invention provides a method for improving sclerotizing of an aspergillus oryzae strain. By adopting the method, sclerotizing, in a slant culture medium, of the aspergillus oryzae strain which is sclerotized can be eliminated completely, mycelia of the sclerotized aspergillus oryzae strain are enabled to have good growing tendency, and enzyme producing capability of the sclerotized aspergillus oryzae strain can be restored. After rejuvenation through the method, fructosyl transferase producing capability of the sclerotized aspergillus oryzae strain can be basically restored, enzyme producing activity reaches 36300U / g (dry weight), biomass of mycelia cultivated through fermentation is restored to reach 90.8g / L, and enzyme producing level of an aspergillus oryzae strain B which is a comparison strain is reached completely. The method is simple to operate, has mild condition and is little in investment, easy to control and good in effect.

Description

technical field [0001] The invention relates to a method for improving the sclerotization phenomenon of molds, in particular to a method for improving the sclerotization phenomenon of Aspergillus oryzae strains. Background technique [0002] During the propagation and cultivation of Aspergillus oryzae strains on slant surfaces, after inoculation of different generations, short hyphae and few spores often appear, and a large number of small, round, firm and hard granular substances appear on the surface of the medium, and the color is white or white. Black, or white spots appear, accompanied by the decrease of the enzyme activity of the strain. The appearance of this phenomenon is usually referred to as the "sclerotinization" of the inclined plane, as shown in the attached figure 1 shown. [0003] In the process of slant culture, the strains, especially the artificially derived mutagenic strains, usually have this phenomenon. The sclerotized slant is difficult to be used a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/69
Inventor 张毅范森王圣海王鹏林晓珊李弘源
Owner SOUTH CHINA UNIV OF TECH
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