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A method for rapidly improving the sclerotization phenomenon of Aspergillus oryzae

A technology of Aspergillus oryzae and sclerotia, which is applied in the field of rapidly improving the sclerotia phenomenon of Aspergillus oryzae strains, can solve the problems of degradation of the ability to absorb and utilize nutrients, genetic variation, decline of the growth ability of strains, etc., and achieves the growth of mycelium. Good effect, low input, recovery of enzyme production capacity

Inactive Publication Date: 2018-05-01
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

[0004] At present, the specific reason for the formation of sclerotia on the slope is not clear, but it is estimated to be related to the decline of the growth ability of the strain, the degradation of the ability to absorb and utilize nutrients, and genetic variation

Method used

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  • A method for rapidly improving the sclerotization phenomenon of Aspergillus oryzae
  • A method for rapidly improving the sclerotization phenomenon of Aspergillus oryzae
  • A method for rapidly improving the sclerotization phenomenon of Aspergillus oryzae

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Embodiment 1

[0033] The Aspergillus oryzae bacterial strain adopted in the present embodiment is:

[0034] Aspergillus oryzae strain A: Sclerotinized fructosyltransferase producing strain Aspergillus oryzae scut209 (Aspergillusoryzae scut209), such as figure 1 shown;

[0035] Aspergillus oryzae strain B: a normal fructosyltransferase production strain preserved in our laboratory. Aspergillus oryzae strain A is a strain of Aspergillus oryzae that has sclerotized after several generations of inoculation. The Aspergillus oryzae strain B is preserved in The Chinese Type Culture Collection Center in Wuhan, China, the collection number is CCTCC NO: M2012106; and it has been disclosed in the authorized invention patent entitled "A strain producing fructosyltransferase and its preparation method", the patent authorization number is: ZL201210374664 .5.

[0036] The mold conventional preservation slant culture medium used in this example is made of the following components by weight: white granula...

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Abstract

The invention provides a method for quickly improving the sclerotinization phenomenon of Aspergillus oryzae strains. On the basis of improving the composition of the medium, the invention adopts the alternate culture method of liquid and solid, so that the operation time is shortened from about one month to one week. Eliminate the phenomenon of mold sclerotization as quickly as possible, and the method is simple in operation, less in investment, easy to master and good in effect. By adopting the method of the invention, the sclerotia phenomenon in the slant culture medium of the Aspergillus oryzae strain can be completely eliminated, the growth of the mycelium is good, and the enzyme production ability of the strain is restored. After desclerotization and rejuvenation experiments, the fructosyltransferase production ability of the original Aspergillus oryzae strain basically recovered to the enzyme production level of the control strain Aspergillus oryzae strain B, and the enzyme activity reached more than 35000U / g. Biomass also recovered to more than 85g / L.

Description

technical field [0001] The invention relates to a method for improving the sclerotization phenomenon of molds, in particular to a method for rapidly improving the sclerotization phenomenon of Aspergillus oryzae strains. Background technique [0002] During the propagation and cultivation of Aspergillus oryzae strains on slant surfaces, after inoculation of different generations, short hyphae and few spores often appear, and a large number of small, round, firm and hard granular substances appear on the surface of the medium, and the color is white or white. Black, or white spots appear, accompanied by the decrease of the enzyme activity of the strain. The appearance of this phenomenon is usually referred to as the "sclerotinization" of the inclined plane, as shown in the attached figure 1 shown. [0003] In the process of slant culture, the strains, especially the artificially derived mutagenic strains, usually have this phenomenon. The sclerotized slant is difficult to b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/69
Inventor 张毅王圣海范森王鹏林晓珊陈子健
Owner SOUTH CHINA UNIV OF TECH
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