Method for quickly improving coring phenomena of aspergillus oryzae

Abstract

The invention provides a method for quickly improving coring phenomena of aspergillus oryzae strains. The method has the advantages that liquid and solid alternate cultivation modes are adopted on the basis of improving composition of cultivation media, so that the operation time can be shortened and is a week from the conventional operation time of a month approximately, and the coring phenomena of aspergillus oryzae can be eliminated as quickly as possible; the method is easy to implement and master and low in input, and good effects can be realized; the coring phenomena of the slant cultivation media for the aspergillus oryzae strains can completely disappear by the aid of the method, mycelia are excellent in growth vigor, and the enzyme producing ability of the strains can be recovered; the fructosyl transferase producing ability of the original aspergillus oryzae strains can be basically recovered and reaches the enzyme producing level of aspergillus oryzae strains B which are comparison strains after de-coring rejuvenation experiments are carried out, the enzyme activity can reach 35000 U / g at least, and the biomass weights of the fermented and cultivated mycelia can be recovered and reach 85 g / L at least.

Description

technical field [0001] The invention relates to a method for improving the sclerotization phenomenon of molds, in particular to a method for rapidly improving the sclerotization phenomenon of Aspergillus oryzae strains. Background technique [0002] During the propagation and cultivation of Aspergillus oryzae strains on slant surfaces, after inoculation of different generations, short hyphae and few spores often appear, and a large number of small, round, firm and hard granular substances appear on the surface of the medium, and the color is white or white. Black, or white spots appear, accompanied by the decrease of the enzyme activity of the strain. The appearance of this phenomenon is usually referred to as the "sclerotinization" of the inclined plane, as shown in the attached figure 1 shown. [0003] In the process of slant culture, the strains, especially the artificially derived mutagenic strains, usually have this phenomenon. The sclerotized slant is difficult to b...

AI Technical Summary

Problems solved by technology

[0004] At present, the specific reason for the formation of sclerotia on the slope is not clear, but it is estimated to be related to the decline of the growth ability of the strain, the degradation of the ability to absorb and utilize nutrients, and genetic variation

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