Method for quickly improving coring phenomena of aspergillus oryzae

A technology of Aspergillus oryzae and Aspergillus oryzae, which is applied in the field of rapidly improving the sclerotinization of Aspergillus oryzae strains, can solve the problems of gene variation, decline of strain growth ability, degradation of ability to absorb and utilize nutrients, etc., and achieve low investment and high production efficiency. Enzyme ability recovery and good mycelium growth

Inactive Publication Date: 2015-12-30
SOUTH CHINA UNIV OF TECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the specific reason for the formation of sclerotia on the slope is not clear, but it is estimated to be related to

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for quickly improving coring phenomena of aspergillus oryzae
  • Method for quickly improving coring phenomena of aspergillus oryzae
  • Method for quickly improving coring phenomena of aspergillus oryzae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The Aspergillus oryzae bacterial strain adopted in the present embodiment is:

[0034] Aspergillus oryzae strain A: Sclerotinized fructosyltransferase producing strain Aspergillus oryzae scut209 (Aspergillusoryzaescut209), such as figure 1 shown;

[0035] Aspergillus oryzae strain B: a normal fructosyltransferase production strain preserved in our laboratory. Aspergillus oryzae strain A is a strain of Aspergillus oryzae that has sclerotized after several generations of inoculation. The Aspergillus oryzae strain B is preserved in The Chinese Type Culture Collection Center in Wuhan, China, the collection number is CCTCCNO:M2012106; and it has been disclosed in the authorized invention patent entitled "A strain producing fructosyltransferase and its preparation method", and the patent authorization number is: ZL201210374664. 5.

[0036] The mold conventional preservation slant culture medium used in this example is made of the following components by weight: white granul...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for quickly improving coring phenomena of aspergillus oryzae strains. The method has the advantages that liquid and solid alternate cultivation modes are adopted on the basis of improving composition of cultivation media, so that the operation time can be shortened and is a week from the conventional operation time of a month approximately, and the coring phenomena of aspergillus oryzae can be eliminated as quickly as possible; the method is easy to implement and master and low in input, and good effects can be realized; the coring phenomena of the slant cultivation media for the aspergillus oryzae strains can completely disappear by the aid of the method, mycelia are excellent in growth vigor, and the enzyme producing ability of the strains can be recovered; the fructosyl transferase producing ability of the original aspergillus oryzae strains can be basically recovered and reaches the enzyme producing level of aspergillus oryzae strains B which are comparison strains after de-coring rejuvenation experiments are carried out, the enzyme activity can reach 35000 U/g at least, and the biomass weights of the fermented and cultivated mycelia can be recovered and reach 85 g/L at least.

Description

technical field [0001] The invention relates to a method for improving the sclerotization phenomenon of molds, in particular to a method for rapidly improving the sclerotization phenomenon of Aspergillus oryzae strains. Background technique [0002] During the propagation and cultivation of Aspergillus oryzae strains on slant surfaces, after inoculation of different generations, short hyphae and few spores often appear, and a large number of small, round, firm and hard granular substances appear on the surface of the medium, and the color is white or white. Black, or white spots appear, accompanied by the decrease of the enzyme activity of the strain. The appearance of this phenomenon is usually referred to as the "sclerotinization" of the inclined plane, as shown in the attached figure 1 shown. [0003] In the process of slant culture, the strains, especially the artificially derived mutagenic strains, usually have this phenomenon. The sclerotized slant is difficult to b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14C12R1/69
Inventor 张毅王圣海范森王鹏林晓珊陈子健
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap