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Germplasm storage method of zinnia elegan tissue culture propagation

A preservation method and germplasm technology, applied in the field of horticultural science, can solve the problems of high cost and large area of ​​germplasm preservation, and achieve the effects of wide sources, reduced growth season restrictions, and reduced labor force and cultivation and preservation space

Active Publication Date: 2015-12-09
GUANGDONG BAILIN GARDEN CONSTR CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of conventional zinnia germplasm preservation methods, provide a zinnia germplasm preservation technology with longer storage period, lower storage cost and reduced variation, and solve the problem of germplasm preservation. Large size and high cost greatly save the cost of raw materials, labor and storage space for cultivation, and the operation is simple, the technology is stable and reliable, and the repeatability is strong, which is very suitable for the preservation of large-scale Zinnia germplasm

Method used

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  • Germplasm storage method of zinnia elegan tissue culture propagation
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  • Germplasm storage method of zinnia elegan tissue culture propagation

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Embodiment

[0036] From 2010 to 2014, Zinnia germplasm Fangfei No. 2 was selected to preserve the germplasm of tissue culture and propagation according to the technical route of the present invention. The detailed steps are as follows:

[0037] 1) Material selection and low temperature pretreatment of explants:

[0038] Select the whole zinnia plant that has grown to the budding stage, is healthy and disease-free, and has the same germplasm as the parent. First, wash off the root soil and other surface debris with tap water, and then use a brush dipped in detergent to wash it once and then Rinse with ultrapure water, immerse the plant roots in 8mg / L KT solution, and pretreat at 2°C for 5 days;

[0039] 2) Vaccination:

[0040] Clean the zinnia with ultra-pure water and transfer it to an ultra-clean workbench, disinfect the surface with 70% alcohol for 15 seconds, rinse twice with sterile water, and then place it in 0.1% mercuric chloride with a few drops of Tween 20 Treat in medium for ...

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Abstract

The invention discloses a germplasm storage method of zinnia elegan tissue culture propagation. The method comprises the following steps: (1) selecting and pretreating an explant at a low temperature; (2) inoculating; (3) inducing and transferring adventitious buds; (4) storing germplasm in a slow growth manner; (5) carrying out the rejuvenation; (6) rooting and transplanting. By adopting the germplasm storage method of the zinnia elegan tissue culture propagation, the storage period of the zinnia elegan germplasm reaches 4 years, the problems of the germplasm storage that the occupied floor area is large, the expense is high and the like can be solved; moreover, the operation is simple, the technology is stable and reliable, the repeatability is high. The method is suitable for storing the zinnia elegan germplasm in a large scale and is an excellent germplasm resource storage method for the zinnia elegan.

Description

technical field [0001] The invention relates to a germplasm preservation method for tissue culture propagation of zinnia, belonging to the field of horticultural science and technology. Background technique [0002] Zinnia, Asteraceae, Zinnia genus, native to North America, Mexico and South America and other places, because of rising steadily, the flowers bloom more and more luxuriantly, and have won many good names, such as Zhuangyuanhong, Huoqiuhua, Zinnia, Backgammon and more. Zinnia likes warmth, is not cold-resistant, and is resistant to drought. It should be sunny, and the tongue-shaped flowers will increase under long-day conditions. Zinnia has a long flowering period and a large amount of flowers. Each plant blooms 30-40 flowers from the first flower to the frost. Most of the excellent varieties are Melaleuca petals, which are extremely rich in flower colors. They are commonly used flowers in summer and autumn flower beds. Mexico is the original origin of zinnia, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 不公告发明人
Owner GUANGDONG BAILIN GARDEN CONSTR CO LTD
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