Method for preparing high-anti-fatigue-action soybean peptide determined by microcalorimetric method and anti-fatigue action evaluation experiment

A technology of anti-fatigue activity and microcalorimetry, which is applied in the direction of plant protein processing, etc., can solve the problems of inability to accurately and quantitatively determine the optimal process of protease hydrolysis reaction, lack of theoretical guidance for protease hydrolysis process control, and complicated test methods. High fatigue function, good water solubility, and accurate experimental results

Inactive Publication Date: 2015-12-09
SHANDONG AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Scholars at home and abroad have done a lot of research on the method of protease hydrolysis to prepare soybean peptides. Most of them determine the optimal reaction temperature, acidity and other conditions by measuring the properties of the product. The test methods are complicated and random, and cannot be determined quantitatively. Optimum Process for Protease Hydrolysis Reaction
As a result, the control of protease hydrolysis process lacks theoretical guidance and is difficult to control.

Method used

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  • Method for preparing high-anti-fatigue-action soybean peptide determined by microcalorimetric method and anti-fatigue action evaluation experiment
  • Method for preparing high-anti-fatigue-action soybean peptide determined by microcalorimetric method and anti-fatigue action evaluation experiment
  • Method for preparing high-anti-fatigue-action soybean peptide determined by microcalorimetric method and anti-fatigue action evaluation experiment

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Preparation of solution:

[0020] Solution ①: Accurately weigh the soybean protein isolate sample, and prepare 10gL of buffer solutions with pH values ​​of 6.70, 7.21, 7.73, 8.21, and 8.73 respectively. -1 soy protein isolate solution.

[0021] Solution ②: The preparation concentration is 0.2gL -1 Trypsin solution.

[0022] 2. The soy protein isolate solution was treated in a water bath at 90°C for 10min;

[0023] 3. Experiments were carried out in 4 ml stainless steel ampoules (titration microcalorimeter). Take 2ml of soy protein isolate solution and put it into a stainless steel ampoule. Wind the connecting rod of the titration microcalorimeter around a thin plastic tube, and the tube contains 1ml of alkaline protease solution. The stirring system was turned off, and the rotating speed was 120 rpm. The trypsin solution was injected into the ampoule with a peristaltic pump, and then the thermal power-time curve was measured. stop. The experiment was repeated ...

Embodiment 2

[0032] 1. Sizing

[0033] Put the protein isolate into a temperature-controlled enzyme reactor tank with a stirrer, add water to adjust the slurry, and the slurry concentration is about 10%;

[0034] 2. Enzyme reaction

[0035] After sizing, add alkaline microbial protease according to 1% of the weight of the substrate protein, add the enzyme while stirring, add alkali at any time, keep the pH value ≈ 8.25, the stirring speed = 120r / min, and the enzymatic hydrolysis temperature is maintained at about 329.5K , after 3 hours of enzymatic hydrolysis, 1:2 of bromelain with alkaline protease was added, and the optimal reaction temperature of bromelain was 314.63K, and the optimal reaction pH was 6.99 for 1h.

[0036] 3. Separation and extraction

[0037] The mixed solution after enzymolysis is inactivated by enzyme treatment, pH 4.5, to precipitate unenzymolyzed protein, the mixed solution is pumped into a separator for solid-liquid separation, and the effluent is all acid-solubl...

Embodiment 3

[0047] Anti-fatigue activity evaluation experiment

[0048] Animals: Mice, healthy Kunming species, body weight (25±2) g, all of SPF grade (specific pathogen-free), provided by Taian Taibang Company.

[0049] Tested product: Example 2 product "Soybean peptide with high anti-fatigue activity"

[0050] Animal grouping and administration:

[0051] Pre-test for 3 days, during the pre-test period, the mice were domesticated and observed to observe their adaptation to the new environment, and to train them to feed at fixed points; the daily supply of feed was determined according to the feeding rate table, and the corresponding feeding situation was made. Adjustment. Feeding follows the principles of fixed-point, timed, quantitative and qualitative. Mice whose physique and exercise capacity were significantly different from the average were eliminated. After the pre-test, the mice were randomly divided into 4 groups with 30 mice in each group: the control group, the low, medium ...

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Abstract

The invention discloses a method for preparing high-anti-fatigue-action soybean peptide determined by a microcalorimetric method and an anti-fatigue action evaluation experiment, and belongs to the technical field of soybean deep processing. The optimal protease for preparing the high-anti-fatigue-action soybean peptide is alkaline protease by adopting the microcalorimetric method according to the Michaelis constant comparison of an enzymatic hydrolysis reaction, and a product anti-fatigue-action evaluation experiment is combined to obtain the method for preparing the high-anti-fatigue-action soybean peptide. The method can be used for effectively improving the anti-fatigue function of a product. Compared with an anti-fatigue soybean peptide produced by a conventional process, high-anti-fatigue-action soybean peptide has the advantages of remarkably improving the exercise tolerance of a body, remarkably reducing accumulation of blood lactic acid after body exercise, remarkably increasing the reserve level of hepatic glycogen and remarkably improving the anti-fatigue capability of a body. The high-anti-fatigue-action soybean peptide has the advantages of no pollution, low cost, simple process, remarkable anti-fatigue function, good water solubility, safety, no toxicity, no side effect and the like, has a relatively high popularization value, can be used for generating relatively high economic benefit and social benefit, and has a broad prospect.

Description

technical field [0001] The invention belongs to the technical field of soybean deep processing, in particular to a preparation method of a soybean peptide with high anti-fatigue activity determined by a microcalorimetry method combined with an anti-fatigue activity evaluation experiment. Background technique [0002] At present, my country's anti-fatigue health care products and functional foods are still in their infancy, and the theoretical basis for the research and development of most products originates from traditional Chinese medicine and health preservation culture. my country's anti-fatigue health care products and functional foods need to be improved in terms of efficacy research, separation and extraction of functional ingredients, improvement of absorption rate, flavor adaptability of products, and product types; suitable for athletes, high-intensity manual workers, special The occupational anti-fatigue requirements of post workers are suitable for the daily anti-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/16A23J3/34
Inventor 董海洲李向阳刘季善张立国吴艳海
Owner SHANDONG AGRICULTURAL UNIVERSITY
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