A strain of Paenibacillus polymyxa wxd 6-4 in wheat rhizosphere and its application
A technology for polymyxa spore-like and wheat, which is applied to wheat rhizosphere Paenibacillus polymyxa WXD6-4 and its application fields, can solve problems such as drug resistance improvement, food safety reduction, environmental pollution, etc., and achieves the goal of promoting plant growth. Effect
Active Publication Date: 2018-09-28
HARBIN NORMAL UNIVERSITY
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Problems solved by technology
At present, chemical methods such as the application of chemical fertilizers and pesticides are mainly used to solve this problem, but they cannot be cured, and will cause environmental pollution, increased drug resistance, changes in soil microbial structure, and reduced food safety.
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[0027] The present invention will be further described in detail below in conjunction with specific drawings and embodiments, which are explanations rather than limitations of the present invention.
[0028] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples were purchased from conventional biochemical reagent stores unless otherwise specified. For the quantitative experiments in the following examples, the experiments were repeated more than three times, and the results were averaged.
[0029] 1. Isolation of Bacillus sp. WXD 6-4
[0030] Weigh 5g of wheat rhizosphere soil cultivated in saline-alkali soil in the laboratory, put it into a conical flask containing glass beads and 45mL of sterile water, vibrate vigorously on a culture shaker for 30min (37°C) and then keep it in a water bath at 80°C for 15min. Then carry out gradient dilution with sterile water and spr...
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The invention discloses wheat rhizosphere paenibacillus polymyxa WXD 6-4, and belongs to the field of biotechnology. The wheat rhizosphere paenibacillus polymyxa WXD 6-4 is classified and named as paenibacillus polymyxa, which is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No. 8997. The wheat rhizosphere paenibacillus polymyxa WXD 6-4 disclosed by the invention can be used for synthesizing IAA and siderophore to dissolve insoluble inorganic phosphorus, so as to promote growth of plants, and fermentation broth can take a good inhibitory effect on cochliobolus sativus, fusarium moniliforme, fusarium oxysporum, botryis cinerea and like plant pathogenic fungi; and the wheat rhizosphere paenibacillus polymyxa WXD 6-4 is of great significance to the prevention and control of wheat root rot, and is suitable for development and utilization as an agricultural fungus.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a wheat rhizosphere Paenibacillus polymyxa WXD 6-4 and its application. Background technique [0002] Wheat is the second largest food crop in the world, after corn. In recent years, the occurrence of wheat root rot has reduced the yield of wheat. At present, chemical methods such as the application of chemical fertilizers and pesticides are mainly used to solve this problem, but they cannot be cured, and will cause environmental pollution, increased drug resistance, changes in soil microbial structure, and reduced food safety. Since microorganisms do not pollute the environment, they can solve the above-mentioned environmental problems caused by chemical and agricultural control, are harmless to humans, and can effectively cure plant diseases, so the research on microbial fertilizers and microbial preparations has become a hot spot. Plant growth promoting rhizobacteria...
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IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C12R1/01
Inventor 郭长虹王晓丹程园园刘佳莉刘莎莎张丹
Owner HARBIN NORMAL UNIVERSITY