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Method for determining polyaromatic hydrocarbons in smokeless tobacco product by utilization of on-line solid phase extraction high performance liquid chromatography

A high-performance liquid chromatography, smokeless tobacco technology, applied in the direction of measuring devices, instruments, scientific instruments, etc.

Inactive Publication Date: 2015-12-09
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, in this method, after the sample is extracted by solvent, it needs to be purified and concentrated by solid-phase extraction column before GC / MS analysis, which consumes more solvents and is easy to cause environmental pollution; the mixed solvent of ethanol and ether established by Zhang Junsong et al. Extraction, purification by neutral alumina column chromatography, and high performance liquid chromatography are also relatively cumbersome methods

Method used

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  • Method for determining polyaromatic hydrocarbons in smokeless tobacco product by utilization of on-line solid phase extraction high performance liquid chromatography
  • Method for determining polyaromatic hydrocarbons in smokeless tobacco product by utilization of on-line solid phase extraction high performance liquid chromatography
  • Method for determining polyaromatic hydrocarbons in smokeless tobacco product by utilization of on-line solid phase extraction high performance liquid chromatography

Examples

Experimental program
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example 1

[0037] Standard product: mixed standard sample of 11 polycyclic aromatic hydrocarbons (the concentration of 11 polycyclic aromatic hydrocarbons is 2.0mg / ml), dissolved in dichloromethane, including: naphthalene, fluorene, phenanthrene, anthracene, fluoranthene, benzo( a) anthracene, benzo(b) fluoranthene, benzo(k) fluoranthene, benzo(a) pyrene, dibenzo(a, h) anthracene, benzo(g, h, i) perylene, AMP company); 1-methylnaphthalene and 2-methylnaphthalene were all purchased from AccuStandard company;

[0038] Reagents: acetonitrile (chromatographically pure, Duksanpurechemicals, Korea), cyclohexane (chromatographically pure, JTBaker, USA). The experimental water was deionized water produced by Milli-Q pure water system.

[0039] Instrument: ThermoUltiMate3000 high performance liquid chromatograph, equipped with six-channel online degasser, double ternary gradient pump, autosampler (with large volume sampling components), column thermostat (with a 2p-6p switching valve, that is, 2...

example 2

[0052] Table 6 Detection results of polycyclic aromatic hydrocarbons in smokeless tobacco products

[0053] compound Content (ng / g) naphthalene 17.8 1-Methylnaphthalene 48.2 2-Methylnaphthalene 71.0 Fluorene 11.8 Philippines N.D. Anthracene 143.9 Fluoranthene N.D. Benz(a)anthracene 33.7 Benzo(b)fluoranthene N.D. Benzo(k)fluoranthene 877.9 Benzo(a)pyrene N.D. Dibenzo(a,h)anthracene N.D. Benzo(g,h,i)perylene N.D.

[0054] Note: N.D. means not detected.

[0055] Example 3:

example 3

[0057] Table 7 Detection results of polycyclic aromatic hydrocarbons in smokeless tobacco products

[0058] compound Content (ng / g) naphthalene 22.5 1-Methylnaphthalene 158.9 2-Methylnaphthalene 0.3 Fluorene 20.8 Philippines N.D. Anthracene 210.5 Fluoranthene 3.8 Benz(a)anthracene 38.9 Benzo(b)fluoranthene 9.0 Benzo(k)fluoranthene 814.5 Benzo(a)pyrene N.D. Dibenzo(a,h)anthracene N.D. Benzo(g,h,i)perylene 4.9

[0059] Note: N.D. means not detected.

[0060] Example 4:

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Abstract

Provided is a method for determining polyaromatic hydrocarbons in a smokeless tobacco product by utilization of on-line solid phase extraction high performance liquid chromatography. Polyaromatic hydrocarbons comprise the following 13 species: naphthalene, 1-methylnaphthalene, 2-methylnaphthalene, fluorene, phenanthrene, anthracene, fluoranthene, benzo(a)anthracene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, and benzo(g,h,i)perylene. The method is characterized in that a triangular flask loaded with cyclohexane is employed to extract polyaromatic hydrocarbons in a smokeless tobacco product, and the content of polyaromatic hydrocarbons in the smokeless tobacco product is analyzed and determined by utilization of on-line solid phase extraction high performance liquid chromatography. The method is advantageous in that the method is employed to detect a content of polyaromatic hydrocarbons in a smokeless tobacco product, the method is rapid and effective, the pre-treatment is simple, the average relative standard deviation is less than 5%, and the average recovery rate of each index is from 83.6%-104.1%. The method has characteristics of rapidness, accuracy, high sensitivity and good repeatability.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical testing of smokeless tobacco products, and in particular relates to the determination technology of polycyclic aromatic hydrocarbons in smokeless tobacco products. Background technique [0002] With the implementation of stricter tobacco control measures, smokeless tobacco products are becoming an important tobacco consumption product. However, there are few studies on the harmful ingredients contained therein. PAHs have been widely concerned in cigarette smoke research, and are considered by the International Agency for Research on Cancer (IARC) as human carcinogens (category 1) or suspected human carcinogens (category 2B), so it is necessary to Analysis and research on polycyclic aromatic hydrocarbons in gas tobacco products. Benzo[a]pyrene (BaP) is the most carcinogenic component in polycyclic aromatic hydrocarbons (PAHs), and accurate determination of BaP is of great significan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张洪非李雪姜兴益罗彦波朱风鹏刘洋庞永强侯宏卫
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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