Establishment of hplc fingerprint of a kiwifruit stem triterpenoid and its fingerprint

Abstract

The invention discloses establishment of kiwi berry steam triterpenes fingerprint and fingerprint thereof. The establishment comprises the following steps: drying stems, leaves, buds, and roots of kiwi fruit in the shape, grinding stems, leaves, buds, and roots into powder, adding methanol into the powder, precisely weighing, carrying out refluxing for 60 minutes under heating, cooling, supplementing the lost weight by methanol, filtering, washing the container and filter residues by methanol for several times, merging the filtrate and washing liquid, evaporating the solution in a rotary evaporator, dissolving the solids in rotary evaporator by methanol, washing the container by methanol, merging the methanol, filtering the merged methanol by a microporous filter membrane with a pore size of 0.45 [mu]m, taking the subsequent filtrate as the test article solution; taking an acetonitrile-formic acid (0.2%) water solution as the mobile phase to carry out high performance liquid chromatography (HPLC), and performing gradient elution to obtain the fingerprint. The test article is easy to manufacture, and the chromatographic conditions can be achieved easily. Furthermore, the stability and repeatability are both good. The method can be used to identify the authenticity, producing area, and quality of branches, leaves, and flowers of kiwi berry and is capable of being used to control the quality.

Description

technical field [0001] The invention belongs to the field of food and drug management, and in particular relates to the establishment of an HPLC fingerprint spectrum of kiwifruit stem triterpenoids and the fingerprint spectrum thereof. Background technique [0002] Fingerprint of traditional Chinese medicine specifically refers to the spectrum or image of the group characteristics of each component of traditional Chinese medicine obtained by modern analysis techniques such as spectrum or chromatography of traditional Chinese medicine samples. As a comprehensive and quantifiable quality control method, traditional Chinese medicine fingerprinting has been recognized by many international medical institutions and has become one of the most effective methods for quality control of Chinese patent medicines and natural medicines. In the "FDA Guidelines on Plant Products" formulated by the US Food and Drug Administration in 1996, it is required to provide corresponding fingerprints...

AI Technical Summary

Problems solved by technology

From the perspective of kiwifruit cultivation, a large number of branches are pruned every winter, and the stems, leaves, and flowers are discarded; therefore, the establishment of fingerprints of active ingredients in branches, leaves, and flowers is a solution to the single quality evaluation method, which is not enough to systematically and completely indicate the overall quality of medicinal materials. quality, it is urgent to explore more advanced and effective quality control methods; and to make full use of kiwifruit resources

[0003] At present, there is no report on the triterpenoid fingerprints of kiwifruit branches, leaves and flowers

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