A method for establishing HPLC fingerprints of kiwifruit stems, leaves, flower buds, and delicious root flavonoids

Abstract

The invention discloses establishment of kiwi berry stem flavonoid HPLC fingerprint and fingerprint thereof. The establishment comprises the following steps: preparing a test article solution; preparing a reference substance solution; taking octadecylsilane chemically bonded silica as the filling agent, acetonitrile as the mobile phase A, and a phosphoric acid solution (0.2%) as the mobile phase B to carry out gradient elution; individually and precisely injecting 10 [mu]L of reference substance solution and 10 [mu]L of test article solution into liquid chromatograph, and carrying out measurement under a detection wavelength of 254 nm and a column temperature of 35 DEG C. According to the invention, branches, leaves, and flowers of kiwi berry are taken as a whole body, and the tiny differences between them can be found out by comparing the common peaks. The test article is easy to manufacture, and the chromatographic conditions can be achieved easily. Furthermore, the stability and repeatability are both good. The method can be used to identify the authenticity, producing area, and quality of branches, leaves, and flowers of kiwi berry and is capable of being used to control the quality.

Description

technical field [0001] The invention belongs to the field of food and drug management, and in particular relates to the establishment of HPLC fingerprints of kiwifruit stem flavonoids and the fingerprints thereof. Background technique [0002] Fingerprint of traditional Chinese medicine specifically refers to the spectrum or image of the group characteristics of each component of traditional Chinese medicine obtained by modern analysis techniques such as spectrum or chromatography of traditional Chinese medicine samples. As a comprehensive and quantifiable quality control method, traditional Chinese medicine fingerprinting has been recognized by many international medical institutions and has become one of the most effective methods for quality control of Chinese patent medicines and natural medicines. In the "FDA Guidelines on Plant Products" formulated by the US Food and Drug Administration in 1996, it is required to provide corresponding fingerprints for plant raw materia...

AI Technical Summary

Problems solved by technology

From the perspective of kiwifruit cultivation, a large number of branches are pruned every winter, and stems, leaves, and flowers are discarded; therefore, the establishment of fingerprints of active ingredients in branches, leaves, and flowers is a solution to the single quality evaluation method, which is not enough to systematically and completely indicate the overall quality of medicinal materials. quality, it is urgent to explore more advanced and effective quality control methods; and to make full use of kiwifruit resources

[0003] Flavonoid fingerprints of kiwifruit branches, leaves and flowers have not been reported so far

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