Method for evaluating small intestine digestibility of amino acid in rumen undegradable protein of sheep fattening feed

Abstract

The invention discloses a method for evaluating the small intestine digestibility of amino acid in rumen undegradable protein of sheep fattening feed. The method comprises the following steps that rumen undegradable residues are sealed in small intestine nylon bags, and then the bags are put in a hydrochloric acid pepsin solution for cultivation; one cultivated small intestine nylon bag is placed in the intestinal tract from the duodenal fistula every 15 min; in 48 h after all the nylon bags are placed in the duodenal fistula, the small intestine nylon bags are collected from manure and cryopreserved after chymus residues are washed with running water; after being collected, all the small intestine nylon bags are washed in a full-automatic washing machine, then dried and weighed, the rest residues are sieved by a 40-mesh sieve, and all samples are used for analysis of the amino acid; the small intestine digestibility of the RUP=(the protein content in the rumen undegradable residues-the protein content in the residues digested by the small intestines)/the protein content in the rumen undegradable residues*100%. The method is accurate and reliable.

Description

technical field [0001] The invention relates to the technical field of feed digestibility, in particular to a method for evaluating small intestinal digestibility of amino acids in rumen undegraded protein in feed for fattening sheep. Background technique [0002] According to the structure and physiological characteristics of animal digestive organs, domestic animals are divided into two categories: ruminants and monogastric animals. There are many types of ruminants in my country, including beef cattle, dairy cows, buffaloes, sheep, goats, camels, etc. Ruminants can use roughage , non-protein nitrogen and other forages that cannot be used by monogastric animals. The main reason is that the stomach of ruminants is compound stomach, which is divided into four parts: rumen, reticulum, omasum and abomasum. , its mucosa has no glandular distribution, which is equivalent to the glandless area of ​​monogastric. The function of the abomasum is the same as that of the normal single ...

AI Technical Summary

Problems solved by technology

[0007] The in vitro three-step method for assessing the intestinal digestibility of amino acids in undegraded protein in the rumen of rams has the following disadvantages: the contents of the fermentation vessel used cannot be moved outside, resulting in the accumulation of fermentation products, causing changes in the environment of rumen microorganisms, thus affecting The stability and accuracy of the measurement results; only the digestibility at a certain time point can be measured, and the dynamic digestibility cannot be measured; due to the specificity of the enzyme, it is difficult to simulate the complex degradation process of proteins by microorganisms in the rumen with a single enzyme or a compound enzyme ; Some of the reagents used have strong rot and toxicity, which are harmful to human body and environment

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