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Preserving fluid of drone semen and preparation method of preserving fluid of drone semen

A preservation method and technology of preservation solution, which are applied in the field of animal genetics and breeding, can solve the problems of limiting the widespread promotion of honey bee drone semen, high requirements for low temperature preservation conditions, low success rate of artificial insemination, etc., so as to achieve no decrease in sperm motility and low requirements for preservation conditions. , the effect of high success rate of artificial insemination

Inactive Publication Date: 2015-12-16
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, low temperature storage requires high requirements and high cost, which limits the widespread promotion of the preservation of bee drone semen
However, when stored at room temperature, the storage time is short, the sperm survival rate after storage is low, and the success rate of artificial insemination is low.

Method used

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  • Preserving fluid of drone semen and preparation method of preserving fluid of drone semen
  • Preserving fluid of drone semen and preparation method of preserving fluid of drone semen
  • Preserving fluid of drone semen and preparation method of preserving fluid of drone semen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, the preparation of the preservation solution of honeybee drone semen

[0049] 1. Preparation of semen storage solution: accurately weigh 0.3 g of glucose, 0.41 g of potassium chloride, 0.21 g of sodium bicarbonate, and 2.43 g of sodium citrate dihydrate with an electronic analytical balance, dissolve them in 50 mL of ultrapure water, and use After stirring evenly with a magnetic stirrer, prepare 100mL base liquid, autoclave at 120°C, and put it in a refrigerator at 2°C-5°C after natural cooling.

[0050] 2. Preparation of semen protection solution: Dissolve 5g of dihydrostreptomycin in 20mL of water to make a stock solution with a final concentration of 25% (100×); configure 1% NaCl solution, autoclave at 120°C for 15 minutes, and wait for cooling Then add dihydrostreptomycin in proportion to make the final concentration 0.25%. The sterilized protection solution is divided into 1.5mL preservation tubes for use;

[0051] Both the semen storage solution an...

Embodiment 2

[0052] Embodiment 2, the preparation of the preservation solution of honeybee drone semen

[0053] 1. Preparation of semen storage solution: Accurately weigh 0.1 g of glucose, 0.2 g of potassium chloride, 0.1 g of sodium bicarbonate, and 1 g of sodium citrate dihydrate with an electronic analytical balance, dissolve them in 50 mL of ultrapure water, and use a magnetic After stirring evenly with a stirrer, prepare 100mL base liquid, autoclave at 120°C, and put it in a refrigerator at 2°C-5°C after natural cooling.

[0054] 2. Preparation of semen protection solution: Dissolve 5g of dihydrostreptomycin in 20mL of water to make a stock solution with a final concentration of 25% (100×); configure 1% NaCl solution, autoclave at 120°C for 15 minutes, and wait for cooling Then add dihydrostreptomycin in proportion to make the final concentration 0.1%. The sterilized protection solution is divided into 1.5mL preservation tubes for use;

[0055] Both the semen storage solution and th...

Embodiment 3

[0056] Embodiment 3, the preparation of the preservation solution of honeybee drone semen

[0057] 1. Preparation of semen storage solution: Accurately weigh 0.5 g of glucose, 0.5 g of potassium chloride, 0.5 g of sodium bicarbonate, and 5 g of sodium citrate dihydrate with an electronic analytical balance, dissolve them in 50 mL of ultrapure water, and use a magnetic After stirring evenly with a stirrer, prepare 100mL base liquid, autoclave at 120°C, and put it in a refrigerator at 2°C-5°C after natural cooling.

[0058] 2. Preparation of semen protection solution: Dissolve 5g of dihydrostreptomycin in 20mL of water to make a stock solution with a final concentration of 25% (100×); configure 3% NaCl solution, autoclave at 120°C for 15 minutes, and wait for cooling Then add dihydrostreptomycin in proportion to make the final concentration 2%. The sterilized protection solution is divided into 1.5mL preservation tubes for use;

[0059] Both the semen storage solution and the ...

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Abstract

The invention belongs to the field of animal genetics and breeding, and discloses preserving fluid of drone semen, a preparation method of the preserving fluid of the drone semen and a method for preserving the drone semen by means of the preserving fluid. The preserving fluid of the drone semen comprises semen storing fluid and semen protection fluid; the semen storing fluid is prepared from glucose, potassium chloride, sodium bicarbonate, sodium citrate and water; the semen protection fluid is prepared from sodium chloride, dihydrostreptomycin and water, the formula is simple, the influence on sperm vitality is small, and the semen protection fluid is suitable for normal temperature preservation and low temperature preservation of the drone semen. The preparation method of the preserving fluid of the drone semen is simple, and the preparation time is short. Due to the fact that the preserving fluid of the drone semen is adopted to preserve the drone semen, the condition requirement is low, the cost is low, the semen vitality is not reduced, and the success rate of artificial insemination is high.

Description

technical field [0001] The invention belongs to the field of animal genetics and breeding, and specifically relates to a semen preservation solution, in particular to a honeybee drone semen preservation solution and a preparation method thereof, and also relates to a method for preserving honeybee semen by using the honeybee drone semen preservation solution. Background technique [0002] Honey bees are social insect groups, and individual types with the same sex but different shapes, body structures, intelligence and behaviors in a bee group are called hierarchical differentiation. In honey bee biology, the class type refers to the two types of queen bee and worker bee that belong to the same female. The different levels of honeybee females are related to their main functions. The queen bee is responsible for laying eggs, and the worker bees are responsible for nursing larvae and supplying food, but they have no fertility. The queen bee is a female bee with fully developed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 刘之光石巍郭海坤陈超陈晓王慧华
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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