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A method for killing psa in kiwi fruit pollen

A technology of kiwi fruit and pollen, applied in botany equipment and methods, biocides, chemicals for biological control, etc., to achieve the effects of ensuring food safety, simple operation, and reducing secondary pollution

Active Publication Date: 2018-10-26
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the prevention and treatment of kiwifruit canker mainly focuses on the screening of control drugs for branches and leaves in the growth period, and the method of killing Psa in pollen has not been reported.

Method used

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  • A method for killing psa in kiwi fruit pollen
  • A method for killing psa in kiwi fruit pollen
  • A method for killing psa in kiwi fruit pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Chlorine dioxide aqueous solution, thimethinone, and can kill three thousand on the bactericidal effect of Psa

[0039] (1) Psa culture

[0040] (2) Three kinds of fungicides and their concentrations

[0041] ClO 2 The concentration of the solution is 20mg / L, 10mg / L, 1mg / L; the concentration of thiothiazide is 500μL / L, 250μL / L, 100μL / L; L.

[0042] (3) The bactericidal effect of three kinds of agents on Psa

[0043] Test group: Use a pipette gun to draw 1 mL of the prepared bacterial suspension into a 10 mL centrifuge tube, then add 4 mL of the above three agents at different concentrations into the centrifuge tube, and mix well. Set the action time gradient of each solution in the bacterial suspension to be 15min, 30min, 45min, 60min, after the action (ClO 2 After the treatment group was treated, take 0.5mL of the sample solution and add 0.5mL neutralizer, neutralize for 10min, and pay attention to the conversion of the dilution factor when the final count) Draw 2...

Embodiment 2

[0050] Study on Effects of Three Chemicals on Pollen Viability

[0051] (1) Concentration and processing time of the three agents

[0052] 10mg / L of ClO 2The solution was treated for 45 minutes; the 500 μL / L thiamethoxam solution was treated for 30 minutes; the 10 mg / L Kede Sanqian solution was treated for 15 minutes.

[0053] (2) Three kinds of chemicals to treat pollen

[0054] Test group: use a pipette gun to draw 1mL of pollen suspension into a 10mL centrifuge tube, then add 1mL of the above three agents at different concentrations into the centrifuge tube, and mix well. Set action time gradient as 15min, 30min, 45min, (ClO 2 Take 0.5mL of the sample solution and add 0.5mL neutralizing agent after the treatment group has acted, neutralize for 10min, pay attention to the conversion of the dilution factor when counting at the end)

[0055] Control group: Use a pipette gun to draw 1ml of pollen suspension into a 10ml centrifuge tube, add 1mL of sterile water to the centri...

Embodiment 3

[0063] ClO 2 Effects of solution-treated pollen on yield and storage quality of kiwifruit

[0064] (1) Concentration and processing time

[0065] ClO 2 The concentration of the solution is 10mg / L, and the treatment time is 45min. After the set time is reached, the neutralizing agent is added immediately.

[0066] (2) Pollination

[0067] Four five-year-old 'Hayward' kiwi fruit trees, healthy and disease-free, with similar tree vigor, were selected in the kiwifruit plantation, and the ClO 2 The pollen is treated with the solution for pollination, and the bag is unpacked for pollination at the full flowering stage, once when the flowers bloom to 60%, 85%, and 100%, respectively, and then bagged immediately after pollination.

[0068] (Control group) For fruit trees No. 1-4, the pollen solution for spraying was prepared normally, and after the preparation, it was left to stand for half an hour and immediately sprayed for fruit trees with a sprayer.

[0069] (3) Investigation...

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Abstract

The invention provides a method for killing Psa (pseudomonas syringae pv.actinidiae) in kiwifruit pollen. By adoption of ClO2 aqueous solution for treating the kiwifruit pollen, Psa in the pollen can be killed effectively while pollen activity is less affected; by killing of the Psa in the kiwifruit pollen, Psa propagation can be effectively controlled to reduce canker, and sustainable development of the kiwifruit industry is guaranteed. The ClO2 aqueous solution treatment method is combined with pollen suspension prepared before liquid pollination, and the ClO2 aqueous solution in an appropriate amount is added into the pollen suspension to kill Psa. Therefore, simplicity in operation, freeness of complex equipment and low cost are realized.

Description

technical field [0001] The invention relates to the technical field of fruit and vegetable disease prevention and control, in particular to a method for killing Psa in kiwi pollen. Background technique [0002] In recent years, the large-scale outbreak of kiwifruit bacterial canker has led to a decline in kiwifruit production, seriously affecting the development of the industry. Kiwifruit canker is a bacterial disease caused by Pseudomonas syingae pv.actinidiae (Psa). With the help of natural conditions such as insects and wind and rain, the pathogens independently infect the shoots of kiwifruit plants, leaf marks of branches, new forks, lenticels of leaves, stomata, etc., or the above tissues with poor nutrition, and then spread into each branch of the fruit tree to cause The whole plant is infected; or spread through pruning wounds and frost cracked wounds. [0003] Gallelli.A et al. (2011) found that canker pathogens existed in kiwifruit pollen in Italy through molecula...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01N59/00A01P1/00
CPCA01H1/02A01N59/00
Inventor 高贵田曹凡朱丹杜莹琳
Owner SHAANXI NORMAL UNIV
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