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Application of ck19 combined with ov6 in preparation of liver cancer molecular typing kit and individualized treatment of liver cancer

A liver cancer, technology for treating liver cancer, applied in the field of medicine

Active Publication Date: 2016-10-19
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, CK19 and OV6 combined as markers for molecular typing of liver cancer and guiding prognosis and personalized treatment have not been reported in the literature

Method used

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  • Application of ck19 combined with ov6 in preparation of liver cancer molecular typing kit and individualized treatment of liver cancer
  • Application of ck19 combined with ov6 in preparation of liver cancer molecular typing kit and individualized treatment of liver cancer
  • Application of ck19 combined with ov6 in preparation of liver cancer molecular typing kit and individualized treatment of liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Detection of OV6 Expression in CK19 Positive Liver Cancer Patient Tissues

[0060] By retrieving the pathological information of liver cancer resected by Eastern Hepatobiliary Surgery Hospital, we found that the positive rate of biliary marker CK19 in liver cancer was about 9%. Then randomly select 102 cases of CK19 + HCC was subjected to immunohistochemical staining for the liver precursor cell marker OV6. The OV6 antibody was purchased from R&D Company at a dilution ratio of 1:100. The antigen retrieval condition was high-pressure alkaline retrieval. It was found that about 65% of them were CK19 + OV6 + HCC (see figure 1 ), this part of CK19 + OV6 + HCC may be derived from hepatic precursor cells, suggesting that the combined application of CK19 and OV6 can guide the molecular classification of liver cancer.

Embodiment 2

[0061] Example 2: CK19 + OV6 + HCC and CK19 + OV6 - Prognosis Analysis of HCC Patients

[0062] For the above 102 cases of CK19 + Liver cancer patients were followed up, and the recurrence time of the patients was recorded (recurrence time refers to the date from the date of operation to the date of diagnosis of tumor recurrence). According to the results of immunohistochemistry, CK19 + Liver cancer is classified as CK19 + OV6 + HCC and CK19 + OV6 - HCC, using the Kaplan–Meier method for CK19 + OV6 + HCC and CK19 + OV6 - A comparative analysis of the disease-free survival of HCC patients found that CK19 + OV6 + HCC patients have shorter disease-free survival and poorer prognosis (see figure 2 ). This result suggests that the combined application of CK19 and OV6 can guide the prognosis of liver cancer patients.

Embodiment 3

[0063] Example 3: Effects of the src inhibitor Dasatinib on malignantly transformed hepatic progenitor cells

[0064] We established the malignantly transformed liver precursor cell line WB-TNF (hepatic precursor cell line WB-F344 was stably formed after continuous stimulation with 1ng / ml TNF-α for 20 weeks), and the construction method was as follows:

[0065] 1. Adhesively culture the liver precursor cell line WB-F344 in DMEM containing 0.5% fetal bovine serum, add 1ng / ml TNF-α to the medium;

[0066] 2. Perform trypsinization and passage of cells every two days, and continue to add 1ng / ml TNF-α to the medium after the cells adhere to the wall;

[0067] 3. Cultured for 20 weeks as described in 1 and 2 to establish the malignantly transformed liver precursor cell line WB-TNF.

[0068] It was found that the level of p-src in the malignantly transformed liver precursor cells was significantly increased, and it had an important regulatory effect on the cells.

[0069] Afterwar...

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Abstract

The invention relates to the technical field of medicine, particularly relates to an application of CK19 and OV6 combination in preparation of a liver cancer molecule typing kit and individualized treatment of liver cancer, and provides application value of the CK19 and OV6 combination as a liver cancer molecule typing marker as well as the application of the CK19 and OV6 combination in preparation of the liver cancer molecule typing kit. A detection method of CK19 and OV6 in liver cancer tissues is further established, CK19 and OV6 immunohistochemical staining and analysis performed on serial slices of the liver cancer tissues show that the CK19<+>OV6<+> liver cancer is derived from hepatic precursor cells and prognosis of a patient is poorer, and accordingly, molecule typing and prognosis evaluation of liver cancer can be guided through CK19 and OV6 combination application. Further, a src inhibitor Dasatinib can remarkably inhibit functions of malignantly transformed hepatic precursor cells, and accordingly, Dasatinib can be used for individualized treatment of CK19<+>OV6<+> liver cancer derived from the hepatic precursor cells.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to the combined application of CK19 and OV6 proteins, in particular to the application of the combination of CK19 and OV6 proteins in the preparation of liver cancer molecular typing kits. The CK19 and OV6 proteins of the present invention and corresponding kits can be used to determine the liver cancer derived from liver precursor cells (ie CK19 + OV6 + Liver cancer), and can be used to assess the prognosis of such patients with liver cancer and guide Dasatinib for personalized treatment of such patients. Background technique [0002] Primary liver cancer is the sixth most common tumor in the world, and its case fatality rate ranks second in the world. Primary liver cancer is mainly divided into three types according to the cell source: hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and mixed liver cancer (CHC). Among them, HCC is the most common type of liv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/57438G01N33/68
Inventor 王红阳丁劲陈程李晓峰
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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