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Cultivation method of tissue cultured seedlings of clematis

A cultivation method and tissue culture seedling technology are applied in the field of cultivating clematis clematis tissue culture seedlings, which can solve the problems of low germination rate of clematis seeds, low rooting rate of seedlings, etc., and meet the requirements of large-scale planting clematis Need, promote growth and reproduction, improve the effect of germination rate

Active Publication Date: 2017-12-22
河池乐康生态农业科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a method for cultivating clematis seedlings, so as to solve the problems of extremely low germination rate of clematis seeds and low seedling rooting rate, and to accelerate the growth and reproduction of clematis seedlings , to meet the needs of large-scale planting of clematis

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for cultivating clematis tissue culture seedlings, the specific steps are as follows:

[0021] A. After the wild clematis capsules are disinfected, the seeds in the clematis capsules are used; the disinfection treatment is to place the clematis capsules in 75% alcohol for 30 seconds on the ultra-clean workbench, Then soak in 0.1% HgCl2 aqueous solution for 8 minutes, and then wash 5 times with sterile water; the seeds are evenly sprinkled on the surface of the induction medium for cultivation. After 25 days, the seeds germinated and formed white protocorms; the formulation of the induction medium was: on the basis of MS medium, 1.5 mg / L of 6-benzylaminopurine (6-BA), 0.1 mg / L of a- Naphthaleneacetic acid (NAA), 200mg / L hydrolyzed casein (CH), 30g / L sucrose, 4.5g / L agar, adjust pH5.4;

[0022] B. Transfer the protocorm to the proliferation medium for cultivation. After 2 months, it develops into seedlings. The seedlings are transferred to the proliferation medi...

Embodiment 2

[0037] A method for cultivating clematis tissue culture seedlings, the specific steps are as follows:

[0038] A. After the wild clematis capsules are disinfected, the seeds in the clematis capsules are used; the disinfection treatment is to place the clematis capsules in 75% alcohol for 30 seconds on the ultra-clean workbench, Then soak in 0.1% HgCl2 aqueous solution for 10 minutes, and then rinse with sterile water for 6 times; the seeds are evenly sprinkled on the surface of the induction medium for cultivation, the cultivation temperature is 25°C, the light intensity is 2000 lux, and the light time is 12 hours / day. On day 35, the seeds germinated and formed white protocorms; the formulation of the induction medium was: on the basis of MS medium, 1.5 mg / L of 6-benzylaminopurine (6-BA), 0.1 mg / L of a- Naphthaleneacetic acid (NAA), 200mg / L hydrolyzed casein (CH), 30g / L sucrose, 4.5g / L agar, adjustment 5.6;

[0039]B. Transfer the protocorm to the proliferation medium for cul...

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Abstract

The invention discloses a method for cultivating tissue-cultured seedlings of A. clematis, relating to the technical field of cultivating tissue-cultured seedlings. 2 days, the seeds germinate to form protocorms; B, transfer the protocorms to the proliferation medium for cultivation, and develop into seedlings after 2 months, and the seedlings are transferred to the proliferation medium for subculture, every 30 days to 35 days Subculture once to obtain clustered buds; C. When the clustered buds grow to a height of 2.5 cm to 3.5 cm, the clustered buds are divided into individual plants and transferred to the rooting medium. After one month, the individual plants grow 1 cm to 3 cm. centimeters of roots, that is, the tissue cultured seedlings of this golden clematis. Compared with the prior art, the present invention promotes seed germination and improves the germination rate of seeds. The seedlings are subcultured to obtain a large number of clustered buds, and the clustered shoots are divided into individual plants and inoculated on the rooting medium to induce rooting. The rooting rate can reach Up to 99% or more.

Description

technical field [0001] The invention relates to the technical field of cultivating tissue-cultured seedlings, in particular to a method for cultivating tissue-cultured seedlings of A. Background technique [0002] Golden clematis, also known as golden thread orchid, golden silk grass, etc., is a perennial herb of the family Orchidaceae, and it is a rare and precious Chinese herbal medicine. The whole herb is used as medicine, which has the effects of cooling and detoxifying, nourishing yin and reducing fire, reducing inflammation and relieving pain. It has a significant effect on unknown swelling, fever, diarrhea and snake bites. used widely. People have been digging a lot without restraint for a long time, and the wild clematis resources are being exhausted day by day, and the artificial cultivation of clematis is becoming more and more extensive, requiring a large amount of clematis seedlings; because the seeds of clematis are small, a capsule contains thousands of seeds....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 唐庆乐
Owner 河池乐康生态农业科技有限公司