Collagen sponge rich in calcium ion on surface and preparation method thereof

A collagen sponge and calcium-enriching technology, used in medical science, absorbent pads, bandages, etc., can solve problems such as limited hemostatic effect, achieve high porosity, promote blood coagulation, and good hemostatic effect.

Active Publication Date: 2016-01-06
北京奥精医疗器械有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for preparing a collagen sponge whose surface is enriched with calcium ions in view of the limited hemostatic effect of existing collagen sponge products

Method used

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  • Collagen sponge rich in calcium ion on surface and preparation method thereof
  • Collagen sponge rich in calcium ion on surface and preparation method thereof
  • Collagen sponge rich in calcium ion on surface and preparation method thereof

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preparation example Construction

[0034] see figure 1 , is a flow chart of the preparation method of the collagen sponge whose surface is enriched with calcium ions of the present invention. Such as figure 1 As shown, the preparation method of the collagen sponge whose surface is enriched with calcium ions provided by the invention mainly comprises the following steps:

[0035] Step S1, the preparation of collagen sponge, specifically includes:

[0036] Step S1-1, dissolving collagen in an aqueous solution of acetic acid to prepare a collagen solution, wherein the concentration of collagen is 0.01-0.03 g / mL;

[0037] Step S1-2, filling the collagen solution obtained in step S1-1 into a mold of a certain shape, and then freeze-drying to obtain a collagen sponge.

[0038] Step S2, the cross-linking of the collagen sponge, specifically includes:

[0039] Step S2-1, prepare 0.005-0.25 wt% glutaraldehyde ethanol solution as a cross-linking agent;

[0040] Step S2-2, soaking the collagen sponge obtained in step...

Embodiment 1

[0051] Step S1-1, dissolving 1 g of collagen in 100 mL of acetic acid aqueous solution to prepare a collagen solution;

[0052] Step S1-2, filling the collagen solution obtained in step S1-1 into a square mold with a side length of 8 cm on the bottom surface, and then freeze-drying to obtain a collagen sponge;

[0053] Step S2-1, prepare 0.01wt% glutaraldehyde ethanol solution as a crosslinking agent;

[0054] Step S2-2, soaking the collagen sponge obtained in step S1-2 in the crosslinking agent solution prepared in step S2-1 for 24 hours to perform crosslinking;

[0055] Step S2-3, taking the collagen sponge obtained in step S2-2 out of the cross-linking agent solution, placing it in a chromatographic column, and washing it with flowing pure water for 60 hours to remove the residual cross-linking agent;

[0056] Step S3-1, preparing a 0.02mol / L aqueous solution of calcium chloride;

[0057] Step S3-2, soaking the collagen sponge washed in step S2-3 in the calcium chloride s...

Embodiment 2

[0062] Step S1-1, dissolving 1.5g of collagen in 100mL of acetic acid aqueous solution to prepare a collagen solution;

[0063] Step S1-2, filling the collagen solution obtained in step S1-1 into a square mold with a side length of 8 cm on the bottom surface, and then freeze-drying to obtain a collagen sponge;

[0064] Step S2-1, prepare 0.05wt% glutaraldehyde ethanol solution as a crosslinking agent;

[0065] Step S2-2, soaking the collagen sponge obtained in step S1-2 in the cross-linking agent solution prepared in step S2-1 for 36 hours to carry out cross-linking;

[0066] Step S2-3, the collagen sponge obtained in step S2-2 is taken out from the cross-linking agent solution, placed in a chromatography column, washed with flowing absolute ethanol for 36 hours, and then washed with pure water for 24 hours to remove residual the cross-linking agent.

[0067] Step S3, binding calcium ions on the surface of the collagen sponge, specifically including:

[0068] Step S3-1, pre...

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Abstract

The invention relates to a collagen sponge rich in calcium ion on the surface and a preparation method thereof. The preparation method includes the following steps: S1, dissolving collagen in a water solution of acetic acid, and performing freeze-drying to obtain a collagen sponge; S2, adopting glutaraldehyde to crosslink the collagen sponge; S3, soaking the collagen sponge in a calcium chloride solution of 0.01-0.1mol / L for 1-12h, taking the collagen sponge out, disposing the same in a chromatographic column for washing for 2-4h, and performing freeze-drying to obtain the collagen sponge rich in calcium ion on the surface. The collagen sponge has high porosity, and can stop bleeding by quickly absorbing water in blood, and calcium ions combined on the surface of the collagen sponge can play an effective role in blood coagulation; in addition, the collagen sponge can be attached on the surface of a tissue organ to play a role in preventing postoperative adhesion.

Description

technical field [0001] The invention relates to the field of biomedical materials, and more specifically relates to a collagen sponge with calcium ion-enriched surface having hemostatic and anti-adhesion functions and a preparation method thereof. Background technique [0002] Intraoperative bleeding is a common symptom in surgical treatment. Blood loss will reduce the fluid in the blood vessels and cause hypotension, which will reduce the oxygen supply of the body tissues and damage the body. Mass blood loss may even cause serious consequences such as hemorrhagic shock, directly endangering human life. Therefore, hemostasis must be performed on the bleeding site in the first place during the operation. [0003] Postoperative adhesion is also one of the important clinical problems to be solved urgently. It has been reported that 50% to 100% of abdominal and pelvic surgery can lead to different degrees of postoperative adhesions, which may lead to serious complications such...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/18A61L15/44
Inventor 崔菡张晓军仇志烨崔福斋
Owner 北京奥精医疗器械有限责任公司
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