Functional cosmetic composition comprising growth factors and amino acids

A cosmetic composition and growth factor technology, applied in cosmetics, cosmetic preparations, medical preparations containing active ingredients, etc., to achieve the effect of improving skin wrinkles, excellent skin wrinkles, and improving skin elasticity

Inactive Publication Date: 2016-01-06
HUMAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In addition, growth factors are proteins that bind to receptors on the cell surface to stimulate cell proliferation and differentiation. Because of their many functions, they stimulate the division of various cells, but sometimes they only act on specific cell type

Method used

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  • Functional cosmetic composition comprising growth factors and amino acids
  • Functional cosmetic composition comprising growth factors and amino acids
  • Functional cosmetic composition comprising growth factors and amino acids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] In Example 1, after adding human growth factors (EGF, FGF, VEGF) to the cell culture solution, the proliferation promoting effect on skin fibroblasts was observed.

[0043] (1) Isolate fibroblasts from human tissues

[0044] The human abdomen skin tissue was taken into a sterilized 10cmbeaker, and washed with phosphorylation buffer solution (D-PBS) of the same skin (1:1). Put 20ml of D-PBS into Cleanbench, grab the tissue with forceps with one hand, and cut the skin tissue into a size less than 2mm with a scalpel. Recover the cut tissue and solution and put 20ml into multiple 50ml conicaltubes for centrifugation. The same amount of 0.15% collagenasetype-1 solution (Invitrogen) was added and reacted at 37°C for 1-2 hours. After washing with D-PBS, centrifugation was performed at 400×g for 5 minutes. Remove the supernatant. After adding 10-40ml of D-PBS to the pellet remaining at the bottom to dissolve the precipitated and agglomerated cells, it is passed through a 70-100m...

Embodiment 2

[0053] In Example 2, the synthesis performance of collagen (collagen) of human fibroblasts was observed in the DMEM medium supplemented with human growth factors and collagen precursors.

[0054] (1) Isolate fibroblasts from human tissues

[0055] The human abdomen skin tissue was taken into a sterilized 10cmbeaker, and washed with phosphorylation buffer solution (D-PBS) of the same skin (1:1). Put 20ml of D-PBS into Cleanbench, grab the tissue with forceps with one hand, and cut the skin tissue into a size less than 2mm with a scalpel. Recover the cut tissue and solution and put 20ml into multiple 50ml conicaltubes for centrifugation. The same amount of 0.15% collagenasetype-1 solution (Invitrogen) was added and reacted at 37°C for 1-2 hours. After washing with D-PBS, centrifugation was performed at 400×g for 5 minutes. Remove the supernatant. After adding 10-40ml of D-PBS to the pellet remaining at the bottom to dissolve the precipitated and agglomerated cells, it is passed t...

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PUM

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Abstract

The present invention relates to a functional cosmetic composition comprising growth factors and amino acids, and more specifically relates to a functional cosmetic composition which is outstandingly effective in skin wrinkle improvement, skin elasticity improvement, skin moisturising, skin regeneration and skin ageing prevention; the composition comprising epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor as the growth factors, and proline, glycine and serine as the amino acids. The functional cosmetic composition comprising the three different growth factors and a collagen synthesis amino acid precursor of the present invention, and a functional cosmetic product comprising same, have outstanding effects in skin wrinkle improvement, skin elasticity improvement, skin moisturising, skin regeneration and skin ageing prevention.

Description

Technical field [0001] The present invention relates to a functional cosmetic composition comprising growth factors (Growthfactors) and amino acids (Aminoacids), in particular to growth factors comprising epidermal growth factor (Epidermalgrowthfactor), fibroblast growth factor (fibroblast growth factor) and vascular endothelial cell growth factor (Vascularendothelialgrowthfactor) ) And functional cosmetic compositions containing proline, glycine, and serine as amino acids that have excellent effects on improving skin wrinkles, improving skin elasticity, skin moisturizing, skin regeneration and skin anti-aging effects. Background technique [0002] Generally speaking, as the age of human skin increases, due to changes in the lipid composition and content of the lipid layer that bears the skin barrier function, its function is drastically reduced, thereby reducing the moisture content of the skin, resulting in dry skin, and causing Various skin lesions. [0003] Excessive fatigue a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61K8/98A61Q19/00
CPCA61K8/64A61K8/44A61K8/4913A61K2800/413A61K2800/5922A61Q19/00A61Q19/007A61Q19/08A61Q19/10
Inventor 李孝宗
Owner HUMAN BIOTECH
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