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Application of Bacillus amyloliquefaciens bam22 in the control of cruciferous clubroot
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A technology of amylolytic spores and Brassicaceae, applied in application, fungicides, chemicals for biological control, etc., can solve the problems of frequent use, complicated use methods, unsatisfactory control effects, etc., and achieve simple use methods , the effect of less dosage of bacterial solution and lower concentration
Active Publication Date: 2018-09-28
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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Problems solved by technology
As above, the cruciferous clubroot biocontrol bacteria currently developed and applied are mainly Bacillus subtilis, Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) in the prevention and control of cruciferous clubroot is still blank, and the control effect of the developed and applied strains is not ideal, and it still needs to be combined with chemical agents to exert a satisfactory control effect. Disadvantages such as complex use methods restrict the application and development of biocontrol bacteria in the prevention and treatment of cruciferous clubroot
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Embodiment 1
[0018] Control effect experiment of Bacillus amyloliquefaciens Bam22 on cruciferous clubroot
[0019] Experimental objects: Rapeseed, Chinese cabbage, broccoli.
[0020] After culturing Bacillus amyloliquefaciens Bam22 for 48 hours, dilute it 500 times, and take the following two methods for prevention and control:
[0021] Method 1: Bacillus amyloliquefaciens Bam22 bacterium liquid is applied to the roots of cruciferous crops in an amount of 100ml per plant, at least three times, and the treatment time is the first day, the seventh day, and the 15th day after sowing. days; Note: The bacterial concentration of the undiluted bacterial solution is about 6-8×10 9 cfu / ml.
[0022] Method 2: Spray the Bacillus amyloliquefaciens Bam22 bacterial liquid at an amount of 180ml per square meter on the cruciferous crops, at least three times, and the treatment time is the first day, the seventh day, and the 14th day after sowing; note : The bacterial concentration of the undiluted bac...
Embodiment 2
[0038] Except that in method one, the application rate of bacterial solution is 80 ml per plant, and in method two, the application rate of bacterial solution is 150 ml per square meter, all the other are consistent with Example 1, and the obtained control effect is 2.0-2.5 lower than that obtained in Example 1 on average. %.
Embodiment 3
[0040] Except that in method one, the application rate of bacterial solution is 90 ml per plant, and in method two, the application rate of bacterial solution is 200 ml per square meter, all the other are consistent with Example 1, and the obtained control effect is equivalent to that of Example 1.
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Abstract
The invention provides an application method of bacillus amyloliquefaciens Bamm22 for preventing and treating plasmodiophora brassicae. The method comprises the following two methods: the method I comprises: carrying out root-irrigation treatment on a cruciferae crop by a bacillus amyloliquefaciens Bamm22 bacterial liquid with the amount of 80-100 ml per time per strain, and carrying out treatment for at least three times, wherein the treatment times are the first day, the seventh day and the fifteenth day after transplanting; the method II comprises: carrying out spraying treatment on the cruciferae crop by a bacillus amyloliquefaciens Bamm22 bacterial liquid with the amount of 150-200 ml per square meter, and carrying out treatment for at least three times, wherein the treatment times are the first day, the seventh day and the fourteenth day after seeding. The bacillus amyloliquefaciens Bamm22 provided by the invention is simple in using method, the application concentration of the bacterial liquid is less and the application dosage of the bacterial liquid is less.
Description
technical field [0001] The invention belongs to the field of prevention and treatment of cruciferous clubroot, and in particular relates to an application method of bacillus amyloliquefaciens Bam22 in the prevention and treatment of cruciferous clubroot. Background technique [0002] Cruciferous clubroot mainly occurs in Europe, the United States, Japan, and southern China. When it is severe, it will cause the failure of cruciferous crops and cause huge troubles to agricultural production. [0003] In recent years, due to the higher and higher requirements for environmental friendliness in the prevention and control of crop diseases, biological control methods have attracted increasing attention from the people, and some strains with good control effects on cruciferous clubroot have been developed. like Bacillus subtilis KB-291, Bacillus subtilis Y10, Bacillus subtilis XF-1, Bacillus subtilis Bs2004, Bacillus subtilis M3. As above, the cruciferous clubroot biocont...
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Application Information
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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P3/00
Inventor 刘勇黄小琴张蕾伍文宪周西全刘红雨
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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