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Polypeptide-modified tannic acid and its preparation method and application

A technology of polypeptide modification and tannin, which is applied in the field of polypeptide-modified tannin and its preparation, can solve problems such as difficult to repair with enamel, low content, poor effect, etc., achieve rich variety, firm adsorption, and promote The effect of tooth enamel remineralization

Active Publication Date: 2019-08-20
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Researchers have used tannic acid alone, and the complex system of tannic acid and ferric ion to repair dentin. The results found that although the former has a certain effect, the effect is not good, while the latter can quickly seal the dental tubules. And induce dentin remineralization to regenerate hydroxyapatite (OhDX, Prajatelistia E, Ju SW, et al..Scientific reports.2015; 5:10884.)
The complexation system of tannic acid and ferric ions mainly relies on the complexation of polyphenols and proteins on dentin to act on the surface of dentin. Compared with dentin, the content of organic components such as collagen in enamel is significantly lower , so the complex system of tannic acid and ferric ion cannot act firmly on the enamel, and it is difficult to be used for the restoration of enamel

Method used

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  • Polypeptide-modified tannic acid and its preparation method and application
  • Polypeptide-modified tannic acid and its preparation method and application
  • Polypeptide-modified tannic acid and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0062] In this embodiment, a preparation method of polypeptide chain modified tannic acid is provided, and its synthetic route is as follows: figure 1 As shown, the steps are as follows:

[0063] (1) Synthesis of acryloyl-modified tannic acid

[0064] ① Prepare the first solution

[0065] Dissolve 4 g of tannic acid in 100 mL of anhydrous dimethylformamide at room temperature and normal pressure to form the first solution, and the concentration of tannic acid in the first solution is 0.04 g / mL;

[0066] ② Prepare the second solution

[0067] Under room temperature, normal pressure condition, triethylamine (TEA) is dissolved in the first kind of solution to form the second kind of solution, and the mol ratio of described triethylamine and tannic acid in the first kind of solution is 1:1;

[0068] ③Synthesis

[0069] Add acryloyl chloride (Acryloyl chloride) dropwise to the second solution under the condition of ice-water bath and argon protection at 0-5°C, react at room tem...

Embodiment 2

[0085] In this embodiment, a method for preparing two polypeptide chain modified tannic acid is provided, the steps are as follows:

[0086] (1) Synthesis of acryloyl-modified tannic acid

[0087] ① Prepare the first solution

[0088] Dissolve 5 g of tannic acid in 100 mL of anhydrous dimethylformamide at room temperature and normal pressure to form the first solution, and the concentration of tannic acid in the first solution is 0.05 g / mL;

[0089] ② Prepare the second solution

[0090] Under room temperature, normal pressure condition, triethylamine is dissolved in the first kind of solution to form the second kind of solution, and the mol ratio of described triethylamine and tannic acid in the first kind of solution is 2.1:1;

[0091] ③Synthesis

[0092]Add acryloyl chloride dropwise to the second solution under the condition of ice-water bath and argon protection at 0-5°C, react at room temperature and normal pressure for 24 hours, and then use deionized water with a mo...

Embodiment 3

[0107] In this example, a method for preparing tannic acid modified by three polypeptide chains is provided, the steps are as follows:

[0108] (1) Synthesis of acryloyl-modified tannic acid

[0109] ① Prepare the first solution

[0110] Dissolve 4.5g of tannic acid in 100mL of anhydrous dimethylformamide at room temperature and normal pressure to form the first solution, and the concentration of tannic acid in the first solution is 0.045g / mL;

[0111] ② Prepare the second solution

[0112] Under room temperature, normal pressure condition, triethylamine is dissolved in the first kind of solution and forms second kind of solution, and the mol ratio of described triethylamine and tannic acid in the first kind of solution is 3.2:1;

[0113] ③Synthesis

[0114] Add acryloyl chloride dropwise to the second solution under the condition of -5~5℃ and argon protection, react at room temperature and normal pressure for 30h, and then dialyze in deionized water with a molecular weight...

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Abstract

The invention provides three types of polypeptide modified tannic acid and a preparation method thereof. Experiments prove that the polypeptide modified tannic acid has a firm adsorption effect on hydroxyapatite and can conduct adsorption and aggregation on the surface of the hydroxyapatite to form a film, the polypeptide modified tannic acid can induce the hydroxyapatite to conduct deposition and crystallization on a worn tooth enamel block in simulated saliva and has the capacity of promoting remineralization of tooth enamel, and when the polypeptide modified tannic acid cooperates with ferric ion for use, the capacity of the polypeptide modified tannic acid for conducting remineralization on the tooth enamel in the simulated saliva can be further promoted; the polypeptide modified tannic acid can effectively prevent bacteria from being stuck to the tooth enamel and prevent bacteria stuck to the tooth enamel from being reproduced on the tooth enamel. The polypeptide modified tannic acid can serve as a tooth enamel in-situ remineralization inducer and a dental implant surface antibacterial material for application.

Description

technical field [0001] The invention belongs to the field of tooth enamel in-situ restoration materials and dental implant surface antibacterial materials, and particularly relates to polypeptide-modified tannic acid and its preparation method and application. Background technique [0002] Human tooth enamel is composed of 96% inorganic materials and 4% organic materials. Hydroxyapatite, which is highly ordered by nanorods and arranged into a well-defined microstructure, is the main inorganic component material of tooth enamel. Studies have shown that amelogenin secreted by ameloblasts is the main material to form the organic components of enamel, accounting for about 90% of all organic matrices in the process of enamel biomineralization. Although tooth enamel is the hardest tissue in the human body, it can still be eroded by bacteria in the mouth or damaged by other mechanical forces. Dental caries is a disease caused by bacteria, mainly manifested as demineralization of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61L27/34A61L27/54A61K6/02
CPCA61K6/80A61L27/34A61L27/54A61L2300/252A61L2300/404A61L2300/606C07K7/06C07K19/00C08L89/00Y02P20/55
Inventor 李建树杨潇杨勃廖逸雪
Owner SICHUAN UNIV
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