Application of trehalose in tobacco leaf curing as a signal molecule in starch metabolism

Abstract

The application of trehalose as a signal molecule in the process of starch metabolism in the tobacco leaf baking process is characterized in that trehalose is atomized and sprayed on the back of the tobacco leaves 12 hours before the tobacco leaves are harvested or before entering the barn after harvesting, which can effectively Improve the activity of amylase in tobacco leaves, increase the content of reducing sugar in tobacco leaves after roasting, and improve the quality of tobacco leaves. The main problem to be solved by the present invention is the high starch content and insufficient reducing sugar content in the cured tobacco leaves in some tobacco areas. Tobacco leaves are mixed and applied before entering the curing barn, so that exogenous substances can increase the amylase activity during the curing process (0~72h), increase the starch degradation rate, and finally achieve the purpose of increasing the reducing sugar content in the cured tobacco leaves and improving the quality of the tobacco leaves. The present invention does not cause the introduction of exogenous tobacco substances due to the use of exogenous reagents, and does not produce the introduction of unknown harmful substances.

Description

technical field [0001] The invention belongs to the field of plant physiology or phytochemical control, specifically relates to the application of trehalose as a signal molecule in the starch metabolism process in the tobacco leaf curing process, and is also a method for improving the amylase activity in tobacco leaves in the tobacco leaf curing process by using in vitro substances . Background technique [0002] The sugar composition of tobacco leaves is an important trait that determines the health of tobacco plants and the quality of tobacco leaves. On the one hand, during the growth and development stage of tobacco plants, sugar accumulation, transport and metabolism largely determine the growth rate of the plant, and have an important impact on the stress and disease resistance of tobacco leaves; on the other hand, sugars in cured tobacco leaves are One of the basic substances of aroma, the content of water-soluble sugar and reducing sugar is an important chemical inde...

AI Technical Summary

Problems solved by technology

During the roasting process of tobacco leaves, the direction of sugar conversion is that sucrose is gradually synthesized from maltose and glucose in the cytoplasm and then transported to the vacuoles, and then the sucrose is hydrolyzed into glucose and fructose, which are mostly completed by enzymatic reactions; if roasting If the humidity in the barn drops too fast, for example, when the humidity in the barn drops below 50%, the decomposition of starch will not be sufficient, and the further decomposition of sucrose will be affected, which will seriously affect the reducing sugar components of the cured tobacco leaves, thereby affecting the quality of smoking (Sun Jingquan, Tang Jingxiang, Ren Sihai, Starch decomposition and sugar conversion pathways and regulation during the curing process of flue-cured tobacco. Anhui Agricultural Sciences, 2014. 45(19): p. 101-103.)

[0005] Reducing sugar is an important material basis for the charred sweetness of tobacco leaves. When the reducing sugar content is low, the tobacco leaves will be "burned but not sweet" when smoking, which will affect the quality and usability of tobacco leaves.

Some researchers have reported that using amylase in microorganisms can effectively degrade the starch content in cured tobacco leaves (CN201210142423.8, CN 201610466228.9), and improve the quality of low-grade tobacco leaves. However, this method may introduce microbial metabolites and affect the smoking evaluation results; in addition, Some researchers sprayed a mixture of various enzymes (protein degrading enzymes, starch degrading enzymes, pectin degrading enzymes, polyphenol oxidase, cellulase) and vitamin C on mature tobacco leaves for curing (CN200510010910.9) , can reduce the content of organic polymer substances in tobacco leaves, improve the quality of first-cured tobacco leaves, and accelerate the alcoholization speed of tobacco leaves

However, the combination of various enzymes in this method will inevitably lead to high cost of the reagent; in addition, the maximum temperature in the curing process is about 60°C, which may seriously affect the enzyme activity sprayed on the surface of tobacco leaves, and has limited effect on the alcoholization of tobacco leaves in the later stage.

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