Application of trehalose in tobacco leaf curing as a signal molecule in starch metabolism

A metabolic process and signaling molecule technology, applied in the fields of application, tobacco, and tobacco preparation, can solve problems affecting the quality of smoking, introducing microbial metabolites, and affecting sucrose decomposition, etc.

Active Publication Date: 2021-09-28
ZHENGZHOU TOBACCO RES INST OF CNTC +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the roasting process of tobacco leaves, the direction of sugar conversion is that sucrose is gradually synthesized from maltose and glucose in the cytoplasm and then transported to the vacuoles, and then the sucrose is hydrolyzed into glucose and fructose, which are mostly completed by enzymatic reactions; if roasting If the humidity in the barn drops too fast, for example, when the humidity in the barn drops below 50%, the decomposition of starch will not be sufficient, and the further decomposition of sucrose will be affected, which will seriously affect the reducing sugar components of the cured tobacco leaves, thereby affecting the quality of smoking (Sun Jingquan, Tang Jingxiang, Ren Sihai, Starch decomposition and sugar conversion pathways and regulation during the curing process of flue-cured tobacco. Anhui Agricultural Sciences, 2014. 45(19): p. 101-103.)
[0005] Reducing sugar is an important material basis for the charred sweetness of tobacco leaves. When the reducing sugar content is low, the tobacco leaves will be "burned but not sweet" when smoking, which will affect the quality and usability of tobacco leaves.
Some researchers have reported that using amylase in microorganisms can effectively degrade the starch content in cured tobacco leaves (CN201210142423.8, CN 201610466228.9), and improve the quality of low-grade tobacco leaves. However, this method may introduce microbial metabolites and affect the smoking evaluation results; in addition, Some researchers sprayed a mixture of various enzymes (protein degrading enzymes, starch degrading enzymes, pectin degrading enzymes, polyphenol oxidase, cellulase) and vitamin C on mature tobacco leaves for curing (CN200510010910.9) , can reduce the content of organic polymer substances in tobacco leaves, improve the quality of first-cured tobacco leaves, and accelerate the alcoholization speed of tobacco leaves
However, the combination of various enzymes in this method will inevitably lead to high cost of the reagent; in addition, the maximum temperature in the curing process is about 60°C, which may seriously affect the enzyme activity sprayed on the surface of tobacco leaves, and has limited effect on the alcoholization of tobacco leaves in the later stage.

Method used

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  • Application of trehalose in tobacco leaf curing as a signal molecule in starch metabolism
  • Application of trehalose in tobacco leaf curing as a signal molecule in starch metabolism

Examples

Experimental program
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Effect test

example 1

[0016] Field experiment: The variety is China Tobacco 100. 12 hours before harvest, 50 tobacco plants with good growth and consistent yellowing in the middle leaves (the 10th leaf position) were selected and sprayed with three concentrations, and distilled water was applied as a control; Once, after harvesting and before entering the barn, select the tobacco field of the plot the day before as a sample, select 15 rods with the same degree of yellowing, and use the same three concentrations to apply the leaves, and apply distilled water for the control.

[0017] Application concentration: 7.5 g / L; 18.9g / L; 28.4g / L three concentrations, corresponding to 20, 50, 75 mM respectively.

[0018] Sampling: On the second day of baking (the temperature of the barn is about 40°C), when the amylase activity is high, enter the barn, take a piece of tobacco leaf from each rod, freeze it in liquid nitrogen, and bring it back to the laboratory to test the amylase activity (Table 2); Tobacco le...

example 2

[0027] Laboratory experiment: The variety is China Tobacco 100, and the tobacco leaf part is the upper tobacco (the top three tobaccos). Select 1,000 tobacco leaves with good growth and consistent yellowing at the top; after harvesting, transport them back to the laboratory as soon as possible. Using the same three trehalose concentrations as in Example 1 plus a clear water control, each concentration was used to treat 100 pieces of tobacco leaves (one cigarette clip), three repetitions, and the control was treated with the same amount of distilled water, three repetitions. After the treatment was completed, the small laboratory oven was used to bake according to the three-stage method. Samples were taken at 24 hours and 48 hours after baking, and the amylase activity was measured after quick freezing in liquid nitrogen. sugar content.

[0028] Table 4 Laboratory experiment of reducing tobacco leaf starch with trehalose

[0029]

[0030] Table 5 Trehalose Low Tobacco Leaf ...

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Abstract

The application of trehalose as a signal molecule in the process of starch metabolism in the tobacco leaf baking process is characterized in that trehalose is atomized and sprayed on the back of the tobacco leaves 12 hours before the tobacco leaves are harvested or before entering the barn after harvesting, which can effectively Improve the activity of amylase in tobacco leaves, increase the content of reducing sugar in tobacco leaves after roasting, and improve the quality of tobacco leaves. The main problem to be solved by the present invention is the high starch content and insufficient reducing sugar content in the cured tobacco leaves in some tobacco areas. Tobacco leaves are mixed and applied before entering the curing barn, so that exogenous substances can increase the amylase activity during the curing process (0~72h), increase the starch degradation rate, and finally achieve the purpose of increasing the reducing sugar content in the cured tobacco leaves and improving the quality of the tobacco leaves. The present invention does not cause the introduction of exogenous tobacco substances due to the use of exogenous reagents, and does not produce the introduction of unknown harmful substances.

Description

technical field [0001] The invention belongs to the field of plant physiology or phytochemical control, specifically relates to the application of trehalose as a signal molecule in the starch metabolism process in the tobacco leaf curing process, and is also a method for improving the amylase activity in tobacco leaves in the tobacco leaf curing process by using in vitro substances . Background technique [0002] The sugar composition of tobacco leaves is an important trait that determines the health of tobacco plants and the quality of tobacco leaves. On the one hand, during the growth and development stage of tobacco plants, sugar accumulation, transport and metabolism largely determine the growth rate of the plant, and have an important impact on the stress and disease resistance of tobacco leaves; on the other hand, sugars in cured tobacco leaves are One of the basic substances of aroma, the content of water-soluble sugar and reducing sugar is an important chemical inde...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A24B3/10
CPCA24B3/10
Inventor 金立锋刘燕翔王燃胡贲郑庆霞张慧刘萍萍李锋李德仑徐国云周会娜
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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