Application of trehalose as signal molecules in starch metabolism process of tobacco leaf baking process

Abstract

Disclosed is an application of trehalose as signal molecules in a starch metabolism process of a tobacco leaf baking process. The application is characterized in that the trehalose is atomized and sprayed on the back surface of tobacco leaves 12 hours before the tobacco leaves are collected or before the collected tobacco leaves enter a baking room, so that the activity of amylase in the tobacco leaves can be effectively improved, the content of reducing sugar of the tobacco leaves after baking is increased, and the quality of the tobacco leaves is improved. The main problem to be solved by the invention is that the content of starch in tobacco leaves baked in certain baking area is high, and the content of reducing sugar is insufficient; according to the method, a proper exogenous substance-trehalose is mainly adopted to be sprayed on the tobacco leaves at a proper concentration after the tobacco leaves are collected and before the tobacco leaves enter the baking room, so that the activity of the amylase is improved by the exogenous substance in the baking process (0-72h), the starch degradation ratio is improved, and finally the purpose of improving the content of reducing sugarin the baked tobacco leaves and improving the quality of the tobacco leaves is achieved. The application does not result in the introduction of a tobacco exogenous substance due to the use of an exogenous reagent, and does not produce an unknown harmful substance.

Description

Technical field [0001] The invention belongs to the field of plant physiology or phytochemical control, and specifically relates to the application of trehalose as a signal molecule in the starch metabolism process during the tobacco leaf curing process, and is also a method for using in vitro substances to improve the amylase activity in the tobacco leaf during the tobacco leaf curing process . Background technique [0002] The sugar composition of tobacco leaves is an important trait that determines the health of tobacco plants and the quality of tobacco leaves. On the one hand, during the growth and development stage of tobacco plants, sugar accumulation, transport and metabolism determine the plant growth rate to a large extent, and have an important impact on the stress and disease resistance of the tobacco leaves; on the other hand, the sugars in the cured tobacco leaves are One of the basic substances of aroma, the content of water-soluble sugar and reducing sugar is an i...

AI Technical Summary

Problems solved by technology

During the roasting process of tobacco leaves, the direction of sugar conversion is that sucrose is gradually synthesized from maltose and glucose in the cytoplasm and then transported to the vacuoles, and then the sucrose is hydrolyzed into glucose and fructose, which are mostly completed by enzymatic reactions; if roasting If the humidity in the barn drops too fast, for example, when the humidity in the barn drops below 50%, the decomposition of starch will not be sufficient, and the further decomposition of sucrose will be affected, which will seriously affect the reducing sugar components of the cured tobacco leaves, thereby affecting the quality of smoking (Sun Jingquan, Tang Jingxiang, Ren Sihai, Starch decomposition and sugar conversion pathways and regulation during the curing process of flue-cured tobacco. Anhui Agricultural Sciences, 2014. 45(19): p. 101-103.)

[0005] Reducing sugar is an important material basis for the charred sweetness of tobacco leaves. When the reducing sugar content is low, the tobacco leaves will be "burned but not sweet" when smoking, which will affect the quality and usability of tobacco leaves.

Some researchers have reported that using amylase in microorganisms can effectively degrade the starch content in cured tobacco leaves (CN201210142423.8, CN 201610466228.9), and improve the quality of low-grade tobacco leaves. However, this method may introduce microbial metabolites and affect the smoking evaluation results; in addition, Some researchers sprayed a mixture of various enzymes (protein degrading enzymes, starch degrading enzymes, pectin degrading enzymes, polyphenol oxidase, cellulase) and vitamin C on mature tobacco leaves for curing (CN200510010910.9) , can reduce the content of organic polymer substances in tobacco leaves, improve the quality of first-cured tobacco leaves, and accelerate the alcoholization speed of tobacco leaves

However, the combination of various enzymes in this method will inevitably lead to high cost of the reagent; in addition, the maximum temperature in the curing process is about 60°C, which may seriously affect the enzyme activity sprayed on the surface of tobacco leaves, and has limited effect on the alcoholization of tobacco leaves in the later stage.

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