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Caragana fabr. plant bark supercritical extract, extraction method and application

A technology of Caragana genus and extract, applied in the field of medicine, can solve problems such as long time consumption and less research on pharmacological effects, and achieve the effects of overcoming long time consumption, significant antifungal effect, and avoiding waste of resources

Inactive Publication Date: 2016-01-13
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, there are few reports on the pharmacological effects of the bark of this genus
Yang Zhongduo et al. isolated 7 compounds from the bark of Caragana ganmeneng, and determined the components with acetylcholinesterase inhibitory activity (Separation and structural identification of acetylcholinesterase inhibitors in Caragana ganmeneng[J], Lanzhou Journal of Polytechnic University, 2015, 41:78-80), the extraction method used is the traditional organic solvent extraction method, which takes a long time and requires the use of toxic solvents such as chloroform
[0005] Caragana (C.korshinskiiKom.) is a perennial deciduous shrub belonging to the genus Caragana. At present, research on it mainly focuses on physiological and ecological functions, feed development and utilization, energy, etc., while there are few studies on pharmacological effects. , so far there is no public report about the bark extract of this plant

Method used

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  • Caragana fabr. plant bark supercritical extract, extraction method and application
  • Caragana fabr. plant bark supercritical extract, extraction method and application
  • Caragana fabr. plant bark supercritical extract, extraction method and application

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Embodiment 1

[0027] The preparation of embodiment 1 extract of the present invention

[0028] (1) Get the fresh bark of Caragana caragana, cut it into pieces, weigh 2.43kg, charge and seal;

[0029] (2) Set supercritical extraction conditions: extraction pressure 25MPa, extraction temperature 50°C, extraction time 3h; (3) Collect 52.8g of extract.

Embodiment 2

[0030] The detection of embodiment 2 extracts of the present invention

[0031] Sample: 0.068g of the extract of the present invention, dissolved to 5ml with n-hexane, filtered and injected (some samples are not dissolved)

[0032] Instruments and testing conditions:

[0033] Agilent7890A-5975C, Hp-5MSUI (30m×0.25mm, 0.25μm)

[0034] SSI:250℃split:1:1

[0035] Column: He, 1.0ml / min

[0036]

[0037] Aux#2280℃

[0038] MS: 71eVsolventdelay: 3.50min

[0039] Scan: 30~600amu(m / z)

[0040] GC-MS analysis results: see figure 1 . Through GC-MS database spectrum comparison, it was confirmed that the signal peak of β-sitosterol was at the retention time of 56.934min, and its content percentage was 2.13%.

[0041] Prove the beneficial effects of the present invention below by drug effect experiment.

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Abstract

The invention provides a caragana fabr. plant bark extract which is prepared by taking caragana fabr. plant bark as a raw material and performing extraction, and the extract comprises, by weight percentage, 1.91-2.42% of beta-sitosterol. Pharmacological experiments prove that the extract is remarkable in antifungal effect and has potential clinical use value. The invention further provides a method for adopting a supercritical CO2 extraction method to extract the caragana fabr. plant bark, and the problems that a conventional organic solvent extraction method is long in time consumption and a poisonous solvent needs to be used are solved.

Description

technical field [0001] The invention relates to a supercritical extract of bark of Caragana genus, its extraction method and application, and belongs to the field of medicine. Background technique [0002] Caragana Fabr. is a deciduous shrub. It is a typical vegetation in the Eurasian steppe plant subregion. It is mainly distributed in the dry area north of the Yellow River Basin in my country. Its cultivated species are generally called Caragana. At present, caragana is one of the important tree species for soil and water conservation and sand-fixing afforestation in Northwest my country, North China, and Northeast China, and it is also a good forage. [0003] Regarding the medicinal value of Caragana genus plants, traditional Chinese medicine believes that this genus plant has the functions of nourishing yin and blood, strengthening the spleen, and promoting blood circulation. , arthritis and gynecological diseases (Jia Minru et al., Chinese Ethnic Medicine [M], Beijing: ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P31/10A61K129/00
Inventor 白波曾智王小艳索有瑞徐恒
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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