Application of Caragana plant bark in the preparation of antifungal drugs

The technology of Caragana caragana and bark is applied in the field of medicine, and can solve the problems such as the antifungal efficacy of the bark of Caragana genus which has not been seen yet, achieve significant antifungal effect of drugs, avoid waste of resources, and expand the scope of resource utilization Effect

Active Publication Date: 2020-05-19
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no relevant literature report on the antifungal effect of Caragana plant bark

Method used

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  • Application of Caragana plant bark in the preparation of antifungal drugs
  • Application of Caragana plant bark in the preparation of antifungal drugs
  • Application of Caragana plant bark in the preparation of antifungal drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation of embodiment 1 bark extract of the present invention

[0022] (1) Get the fresh bark of Caragana caragana, cut it into pieces, weigh 2.43kg, charge and seal;

[0023] (2) Set supercritical extraction conditions: extraction pressure 25MPa, extraction temperature 50°C, extraction time 3h, collect 52.8g of supercritical extract;

[0024] (3) Collect the slag obtained after the extraction in step (2), heat and reflux extraction with 70% v / v ethanol aqueous solution, 2h / time, extract 3 times, filter, combine the filtrates, and concentrate under reduced pressure to obtain the extract;

[0025] (4) The extract obtained in step (3) was dissolved in water, extracted with ethyl acetate, and 37.27 g of the ethyl acetate extract was collected.

Embodiment 2

[0026] The detection of embodiment 2 bark supercritical extract of the present invention

[0027] Sample: 0.068g of the extract of the present invention, dissolved to 5ml with n-hexane, filtered and injected (some samples are not dissolved)

[0028] Instruments and testing conditions:

[0029] Agilent 7890A-5975C, Hp-5MS UI (30m×0.25mm, 0.25μm)

[0030] SSI:250℃ split:1:1

[0031] Column: He, 1.0ml / min

[0032] Oven:

[0033] Aux#2 280℃

[0034] MS: 71eV solvent delay: 3.50min

[0035] Scan: 30~600amu(m / z)

[0036] GC-MS analysis results: see figure 1 . Through GC-MS database spectrum comparison, it was confirmed that the signal peak of β-sitosterol was at the retention time of 56.934min, and its content percentage was 2.13%.

[0037] Prove the beneficial effects of the present invention below by drug effect experiment.

experiment example 1

[0038] The antibacterial experiment of experimental example 1 bark supercritical extract of the present invention

[0039] The following is that the bark supercritical extract prepared by embodiment 1 is carried out drug efficacy test, as follows:

[0040] (1) Activity experiment - inhibition zone

[0041] SDA medium: peptone 10g, glucose 20g, agar 18g, pure water 1L.

[0042] Strain activation: Invert the prepared SDA medium on the slope, use an inoculation needle to pick an appropriate amount of Trichophyton mentagrophytes mycelia to inoculate on the slope, culture at 33°C for 3-5 days, and set aside.

[0043] Inhibition zone:

[0044] ① Configure SDA medium, sterilize at 121°C for 30 minutes, and pour it into a plate for later use;

[0045] ② Take the activated bacteria and prepare the concentration of 10 with pure water 7 ~10 8 Individual / mL bacterial suspension;

[0046] ③ Dip the bacterial suspension with a sterilized cotton swab, and apply it evenly on the prepare...

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Abstract

The invention provides application of caragana plant bark in antifungal drug preparation, the resource utilization range of the caragana plants is widened, and resource waste is avoided. Pharmacological experiments prove that a drug is significant in antifungal effect and has the potential clinical using value.

Description

technical field [0001] The invention relates to a new application of bark of Caragana genus, belonging to the field of medicine. Background technique [0002] Caragana Fabr. is a deciduous shrub. It is a typical vegetation in the Eurasian steppe plant subregion. It is mainly distributed in the dry area north of the Yellow River Basin in my country. Its cultivated species are generally called Caragana. At present, caragana is one of the important tree species for soil and water conservation and sand-fixing afforestation in Northwest my country, North China, and Northeast China, and it is also a good forage. [0003] Regarding the medicinal value of Caragana genus plants, traditional Chinese medicine believes that this genus plant has the functions of nourishing yin and blood, strengthening the spleen, and promoting blood circulation. , arthritis and gynecological diseases (Jia Minru et al., Chinese Ethnic Medicine [M], Beijing: China Medical Science and Technology Press, 200...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61P31/10
Inventor 索有瑞曾智白波胡娜尤进茂
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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