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A kind of cik cell cryopreservation medium

A cryopreservation solution and cell technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of low CIK cell viability, large amount of FBS, loss of surface antigens, etc., and achieve good proliferation ability and killing Activity, maintenance of cell viability, good cell viability effect

Active Publication Date: 2018-01-05
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CIK cells cryopreserved with the above-mentioned cryopreservation solution have a low survival rate after recovery, and the surface antigen is seriously lost, which affects the therapeutic effect; in addition, the amount of FBS in the above-mentioned cryopreservation solution is large and the cost is high

Method used

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  • A kind of cik cell cryopreservation medium
  • A kind of cik cell cryopreservation medium
  • A kind of cik cell cryopreservation medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Slowly add 0.5ml DMSO, 1ml grape seed extract solution, 1ml raffinose solution and 2ml human serum albumin into 5.5ml FBS, mix well and store in a 4°C refrigerator for later use. The volume percent contents of DMSO, grape seed extract solution, raffinose solution, human serum albumin, and FBS in the frozen storage solution of this embodiment are respectively 5%, 10%, 10%, 20% and 55%. The content of raffinose is 2mg / ml, and the content of raffinose is 5mg / ml.

Embodiment 2

[0038] Slowly add 1ml DMSO, 1ml grape seed extract solution, 2ml raffinose solution and 1.5ml human serum albumin into 4.5ml FBS, mix well and store in a 4°C refrigerator for later use. The volume percent contents of DMSO, grape seed extract solution, raffinose solution, human serum albumin, and FBS in the frozen storage solution of this embodiment are respectively 10%, 10%, 20%, 15% and 45%. The content of raffinose is 2mg / ml, and the content of raffinose is 10mg / ml.

Embodiment 3

[0040] Slowly add 1ml DMSO, 0.5ml grape seed extract solution, 1.5ml raffinose solution and 1ml human serum albumin into 6ml FBS, mix well and store in a 4°C refrigerator for later use. The volume percent contents of DMSO, grape seed extract solution, raffinose solution, human serum albumin, and FBS in the freezing solution of this embodiment are respectively 10%, 5%, 15%, 10% and 60%. The content of raffinose is 1mg / ml, and the content of raffinose is 7.5mg / ml.

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Abstract

The invention relates to CIK cell cryopreservation liquid. The CIK cell cryopreservation liquid is prepared from, by volume and concentration, 5-10% of DMSO, 1-2 mg / ml of grape seed extracts, 5-10 mg / ml of raffinose, 10-20% of human albumin, 40-70% of FBS and the balance PBS. Due to the fact that raffinose, grape seed extracts and human albumin are added, cell survival time is prolonged, and the cellular structure is prevented from being damaged by free radicals. By the adoption of the CIK cell cryopreservation liquid, cell viability can be better maintained, loss of cell surface antigens is reduced, and cells still have high multiplication capacity and killing activity after recovery.

Description

technical field [0001] The invention relates to the field of cell cryopreservation, in particular to a CIK cell cryopreservation solution. Background technique [0002] CIK cells are cytokine-induced killer cells (Cytokine-Induced Killer, CIK), which is a new type of immunocompetent cells. CIK has strong proliferation ability, strong cytotoxicity, and certain immune characteristics. The killing of tumor cells by CIK cells is not restricted by MHC, that is, it is not limited by the type of cancer tissue, and can automatically recognize and kill tumor cells in a wide range of parts in the human body. The number of CIK cells in the peripheral blood of normal people only accounts for 1-5% of the number of lymphocytes, and the reduction in the number and function of CIK cells is closely related to the occurrence and development of tumors. [0003] Today, CIK cell therapy is considered to be the first choice for a new generation of anti-tumor adoptive cell immunotherapy. The the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 陈海佳王一飞葛啸虎曾维杰
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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