A kind of cik cell cryopreservation medium
A cryopreservation solution and cell technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of low CIK cell viability, large amount of FBS, loss of surface antigens, etc., and achieve good proliferation ability and killing Activity, maintenance of cell viability, good cell viability effect
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Embodiment 1
[0036] Slowly add 0.5ml DMSO, 1ml grape seed extract solution, 1ml raffinose solution and 2ml human serum albumin into 5.5ml FBS, mix well and store in a 4°C refrigerator for later use. The volume percent contents of DMSO, grape seed extract solution, raffinose solution, human serum albumin, and FBS in the frozen storage solution of this embodiment are respectively 5%, 10%, 10%, 20% and 55%. The content of raffinose is 2mg / ml, and the content of raffinose is 5mg / ml.
Embodiment 2
[0038] Slowly add 1ml DMSO, 1ml grape seed extract solution, 2ml raffinose solution and 1.5ml human serum albumin into 4.5ml FBS, mix well and store in a 4°C refrigerator for later use. The volume percent contents of DMSO, grape seed extract solution, raffinose solution, human serum albumin, and FBS in the frozen storage solution of this embodiment are respectively 10%, 10%, 20%, 15% and 45%. The content of raffinose is 2mg / ml, and the content of raffinose is 10mg / ml.
Embodiment 3
[0040] Slowly add 1ml DMSO, 0.5ml grape seed extract solution, 1.5ml raffinose solution and 1ml human serum albumin into 6ml FBS, mix well and store in a 4°C refrigerator for later use. The volume percent contents of DMSO, grape seed extract solution, raffinose solution, human serum albumin, and FBS in the freezing solution of this embodiment are respectively 10%, 5%, 15%, 10% and 60%. The content of raffinose is 1mg / ml, and the content of raffinose is 7.5mg / ml.
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