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A kind of bacillus licheniformis and microbial agent and their application in the prevention and treatment of plant cropping disease

A technology of bacillus licheniformis and microbial agents, applied in the field of agricultural biology, to achieve the effect of improving the level of prevention and control

Active Publication Date: 2018-08-17
BEIJING HUIMINDA TECH DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, experiments have shown that the ability of the strains in the bacterial agent to prevent and control cropping disease needs to be further improved.

Method used

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  • A kind of bacillus licheniformis and microbial agent and their application in the prevention and treatment of plant cropping disease
  • A kind of bacillus licheniformis and microbial agent and their application in the prevention and treatment of plant cropping disease
  • A kind of bacillus licheniformis and microbial agent and their application in the prevention and treatment of plant cropping disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] This example is used to illustrate the cultivation of Bacillus licheniformis of the present invention and the preparation of microbial inoculum.

[0043] The slant strain of Bacillus licheniformis (Bacillus licheniformis) with the preservation number of CCTCC NO: M2013495 was placed on an LB plate (containing 10 g / L of peptone, 5 g / L of yeast extract, 5 g / L of NaCl and 3 g / L of agar sugar, pH value 7.4, the same below), and cultured at 37°C for 15 hours, picked a single colony from the plate, and inoculated it in 25mL of LB liquid medium (containing 10g / L peptone, 5g / L Yeast extract and 5g / L NaCl, pH value 7.4, hereinafter the same), cultivated on a shaker at 250r / min at 37°C for 15 hours to obtain primary seeds, and added 20mL of primary seeds to 500mL of LB liquid medium In 250r / min shaker at 37°C for 15 hours to obtain secondary seeds, 2L of secondary seeds were added to 50L of LB liquid medium, stirred at 250r / min at 37°C and 10m 3 / h ventilation for 15 hours to ob...

Embodiment 2

[0087] This example is used to illustrate the cultivation of Bacillus licheniformis and Bacillus subtilis of the present invention and the preparation of microbial inoculum.

[0088] The slant strain of Bacillus licheniformis (Bacillus licheniformis) with the preservation number of CCTCC NO: M2013495 was placed on an LB plate (containing 10 g / L of peptone, 5 g / L of yeast extract, 5 g / L of NaCl and 3 g / L of agar sugar, pH value 7.4, the same below), and cultured at 37°C for 15 hours, picked a single colony from the plate, and inoculated it in 25mL of LB liquid medium (containing 10g / L peptone, 5g / L Yeast extract and 5g / L NaCl, pH value 7.4, hereinafter the same), cultivated on a shaker at 250r / min at 37°C for 15 hours to obtain primary seeds, and added 20mL of primary seeds to 500mL of LB liquid medium , cultivated on a shaker at 250 r / min at 37°C for 15 hours to obtain secondary seeds of Bacillus licheniformis.

[0089] The slant strain of Bacillus subtilis (Bacillus subtilis...

Embodiment 3

[0093] The microbial agent was prepared according to the method of Example 2, except that 1.2 L of secondary seeds of Bacillus licheniformis and 1.2 L of secondary seeds of Bacillus subtilis were mixed, and then tertiary expansion cultivation was carried out.

[0094] In this embodiment, the CFU ratio of the Bacillus licheniformis and the Bacillus subtilis in the culture medium measured by the nucleic acid quantification method based on 16S rDNA is 1:0.9.

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Abstract

The invention provides bacillus licheniformis with the preservation number being CCTCC No. M2013495. The invention further provides a microbial agent containing the bacillus licheniformis, other strains and auxiliaries. The invention also provides application of the bacillus licheniformis or the microbial agent to the prevention and treatment of plant continuous cropping. By the technical scheme, the prevention and treatment level of the plant continuous cropping can be increased greatly.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a bacillus licheniformis, a microbial agent and their application in the prevention and treatment of plant cropping disease. Background technique [0002] In recent years, with the increasingly serious situation of desertification and desertification in the world, the arable land has become less and less, and the contradiction of more people and less land in our country has become more prominent. Increasing the multiple cropping index (that is, using double cropping) is the only way to solve the problem. However, the ensuing cropping diseases such as soil-borne diseases caused large-scale production reduction and extinction of crops. Continuous cropping makes many crops such as leguminous plants, melons, vegetables, strawberries and some Chinese herbal medicines. Poor growth, which in turn leads to crop blight, leaf blight, viral diseases and other hazards, seriously af...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/10
Inventor 郝元福
Owner BEIJING HUIMINDA TECH DEV CENT