Time-resolved fluoroimmunoassay kit for detecting adprin as well as detecting method of time-resolved fluoroimmunoassay kit
A time-resolved fluorescence and adefuran technology, which can be used in measurement devices, analytical materials, instruments, etc., can solve the problem of no adefurine, etc., and achieve the effects of simple operation, wide application and strong specificity.
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[0023] Example
[0024] 1. Preparation of immunogen and coating
[0025] The synthesis of the immunogen of the present invention (Adfuroline-BSA): accurately weigh 324 mg of Adfuroline and dissolve it in 2mL N,N-dimethylformamide, add the γ-aminobutyric acid solution dropwise under stirring, and stir to react Adjust the pH of the reaction solution to about 10 for 3 hours. Centrifuge to remove sediment. The above reaction was added dropwise to the BSA solution (320mg BSA dissolved in 5mL saline), and then added N-hydroxysuccinimide (NHS) 23mg, N,N-dicyclohexylcarbodiimide (DCC) 45.4mg, 4 React overnight at °C, centrifuge to remove the precipitate, take the supernatant and dialyzed with phosphate buffered saline (PBS) for 3 days, replace the dialysate every 6 hours, lyophilize the resulting product, and store at -20 °C for later use.
[0026] Synthesis of the coating source (Adfuroline-OVA): In the above reaction, after replacing BSA with OVA, the reaction conjugate Adfuroline-OVA i...
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