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On-line plant glucose detection method and device based on microelectrode biosensing technology

A microelectrode and glucose technology, applied in the field of electrochemistry, can solve the problems of complex sample processing, time-consuming, and inability to directly apply plants, etc., and achieve the effect of simple detection conditions

Active Publication Date: 2016-02-10
BEIJING RES CENT FOR INFORMATION TECH & AGRI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of method needs to sample the plant material in vitro, and calculate the content in the plant by detecting the concentration of glucose in the extract, which requires high purity of the sample extraction. Momentary concentration cannot be used for dynamic analysis and long-term follow-up monitoring
[0004] In order to realize the research on plant growth and environmental adaptability, it is necessary to carry out in-situ dynamic detection in vivo. Currently, the glucose in vivo detection products on the market are mainly aimed at animals or human bodies. Because plants have thin leaves, less tissue fluid, and lignified stems, etc. The above-mentioned detection products cannot be directly applied to plants, so it is urgent to develop an online glucose detection method and device for plant tissues

Method used

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  • On-line plant glucose detection method and device based on microelectrode biosensing technology
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  • On-line plant glucose detection method and device based on microelectrode biosensing technology

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Experimental program
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Effect test

Embodiment 1

[0027] The preparation of embodiment 1 glucose oxidase microelectrode

[0028] The glucose oxidase microelectrode prepared in this example is 5 cm long, and its tip part is 10 mm long; the base electrode material of the microelectrode is a silicon wafer, and the microelectrode has a three-electrode system, including Ag / AgCl reference electrode, platinum Counter electrode and gold working electrode modified by glucose oxidase. ( figure 1 )

[0029] Among them, gold working electrode 2, Ag / AgCl reference electrode 3, and platinum counter electrode 4 are sequentially arranged on the tip of silicon chip substrate 1; the above three electrodes.

[0030] The length of the silicon wafer base is 5 cm, the thickness is 1.5 mm, and the length of the tip part is 4 mm; the exposed tip part of the silicon wafer base 1 is provided with a gold working electrode 2, an Ag / AgCl reference electrode 3, and a platinum counter electrode 4 in sequence. ; The other end of the silicon wafer base i...

Embodiment 2

[0042] Example 2 Real-time online detection of glucose content in potato stem samples based on microelectrode biosensing technology

[0043] After the glucose oxidase microelectrode prepared in Example 1 was cleaned with deionized water, three parts of standard concentration (0.1mmol / L, 1mmol / L, 5mmol / L) glucose solutions were detected respectively earlier, and electrochemical calibration was carried out to obtain the glucose concentration After plotting the relationship with the response peak current, obtain the slope. When the deviation between the slope and the slope of the working curve is within 15%, it is considered that the enzyme activity is normal and the electrode can work normally.

[0044] Insert the cleaned and calibrated microelectrode into the potato stem tissue to be tested, and connect to the electrochemical workstation for cyclic voltammetry scanning for 5 minutes to obtain a stable cyclic voltammetry curve. The obtained response peak current value is substitu...

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Abstract

The invention provides an on-line plant glucose detection method based on the microelectrode biosensing technology. The method comprises the steps of inserting a glucose oxidase microelectrode into a to-be-detected plant tissue, and connecting an electrochemical workstation for cyclic voltammetry scan, so as to achieve the on-line glucose detection of the plant tissue. The invention also provides a device used for achieving the detection method. The device comprises the electrochemical workstation and the glucose oxidase microelectrode which are connected through a conducting wire. According to the on-line plant glucose detection method and the device, the real-time detection of the plant glucose content can be achieved, the detected part of the plant does not need to be separated from the plant body, and has no destructive damage; the vivo detection can be carried out only through surface cleaning and tissue fixing, and continuous results are obtained for dynamic analysis; the detection device provided by the invention is convenient to carry and simple in detection condition, can be operated in the field, and achieves continuous on-line detection.

Description

technical field [0001] The invention relates to the field of electrochemistry, in particular to a plant online glucose detection method and device based on microelectrode biosensing technology. Background technique [0002] Glucose is a monosaccharide, a hexose containing aldehyde groups. It widely exists in plants and is the main component of some sugars (such as sucrose, maltose, starch, cellulose, etc.), and is the main energy substance in plants. Glucose is the product of photosynthesis and the substrate of respiration. It provides energy for plant growth and development and can enhance plant stress resistance. Glucose metabolism is the center of the whole plant metabolism, which communicates the metabolism of proteins, lipids and secondary products. [0003] At present, in the research of plant physiology, colorimetric method, DNS reagent reducing sugar determination method, liquid chromatography and so on are mostly used for glucose detection. This type of method ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
Inventor 胡叶王成王晓冬侯佩臣何璐璐周航任鹏路文超宋鹏潘大宇
Owner BEIJING RES CENT FOR INFORMATION TECH & AGRI
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