In vitro cultured calculus bovis aqueous solution and preparation method thereof
A technology for in vitro cultivation of bezoar and aqueous solution, which is applied in the direction of medical formula, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc. It can solve the problems that the active ingredients cannot be fully absorbed and exert their efficacy, and the way of administration is single. , to achieve the effect of improving solubility and promoting absorption
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example 1
[0016] Grind the bezoar cultivated in vitro into fine powder, pass through a 200-mesh sieve, and set aside; add 5 g of sorbitol and 1 g of essence to 1 L of purified water in sequence, stir, dissolve, and mix well; add 10 g of surfactant poloxamer (PluronicF68) Add it to the above mixed solution; use 1mol / L NaOH to adjust the pH value of the solution to 9.8; then add 10g of sieved in vitro cultured bezoar fine powder to the above mixed solution, and use a homogenizer to stir rapidly for 24h , mix well; centrifuge under the condition of 4000r / min for 15min, take the supernatant, place it at 2-6°C for 5h at low temperature, and then filter; obtain a clear brownish-yellow in vitro cultured bezoar aqueous solution.
example 2
[0018] Grind the bezoar cultivated in vitro into fine powder, pass through a 200-mesh sieve, and set aside; add 8 g of sorbitol and 1.5 g of essence to 1 L of purified water in sequence, stir, dissolve, and mix well; add the surfactant poloxamer (Pluronic F68) Add 15g to the above mixed solution; adjust the pH value of the solution to 9.5 with 1mol / L NaOH; then add 15g of sieved in vitro cultured bezoar fine powder to the above mixed solution, and stir rapidly with a homogenizer 24h, mix well; centrifuge under the condition of 4000r / min for 20min, take the supernatant, put it at 2-6°C for 5h, and then filter to obtain a clear brown yellow in vitro cultured bezoar aqueous solution.
example 3
[0020] Grind the bezoar cultivated in vitro into fine powder, pass through a 200-mesh sieve, and set aside; add 7 g of sorbitol and 1.5 g of essence to 1 L of purified water, stir, dissolve, and mix well; add 8010 g of surfactant Tween to the above-mentioned In the mixed solution; use 0.5 mol / L sodium carbonate to adjust the pH value of the solution to 9; then add 15 g of sieved in vitro cultured bezoar fine powder to the above mixed solution, and use a homogenizer to stir rapidly for 36 hours and mix well Centrifuge at 3000r / min for 20min, take the supernatant, place it at 2-6°C for 6h, and then filter it to obtain a clear brown-yellow in vitro cultured bezoar solution.
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