Culture medium additive for inducing taxus chinensis cells to produce docetaxel semisynthesis precursor

Abstract

The invention provides a culture medium additive for inducing taxus chinensis cells to produce a docetaxel semisynthesis precursor. The additive is prepared from thia-diazonium phenylurea, gibberellin, povidone, quercetin, peptone, yeast powder, scutellaria baicalensis, azedarach, hyoscyamus niger, psammosilene tunicoides and the like. In the mode that an elicitor is added into the culture medium, the autophage mode of the taxus chinensis cells is interfered, and therefore the taxus chinensis cells are induced to produce the docetaxel semisynthesis precursor namely 10-deacetylated baccatin III. It is found through experiments that after the additive is added in to cell culture liquid, the secretion amount of 10-deacetylated baccatin III by the taxus chinensis cells is substantially increased, and cell culture density can be increased to a certain degree. The technical thought is clear, the technical scheme is reasonable, and the culture medium additive has good large-scale application prospects.

Description

technical field [0001] The invention relates to the technical field of cell engineering, in particular to a medium additive for inducing yew cells to produce a semisynthetic precursor of docetaxel. Background technique [0002] Paclitaxel is a diterpenoid extracted from the bark of the Taxus genus Taxus. It is a new type of microtubule stabilizer with unique anticancer activity. It is considered by the National Cancer Institute of the United States to be the most important progress in tumor chemotherapy in the past 15 to 20 years. As a second-line treatment drug for advanced ovarian cancer, it has been approved for marketing in more than 40 countries so far, and has shown encouraging curative effects in the treatment of breast cancer, lung cancer, leukemia, gastrointestinal cancer and vascular restenosis after interventional therapy. . Paclitaxel has limited its clinical application due to the lack of resources and low water solubility. [0003] Docetaxel contains a taxan...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a medium additive for inducing yew cells to produce semi-synthetic precursors of docetaxel, so as to solve the problem of 10-deacetylbaccatin in yew cells in the prior art. The technical problem of low secretion of Ⅲ