Elicitor composition for producing paclitaxel from Taxus chinensis cells

A technology of yew cells and elicitors, applied in the field of cell engineering, can solve the problem of low paclitaxel secretion, achieve the effect of improving the current situation of large-scale production and broad application prospects

Inactive Publication Date: 2016-04-20
天津市博爱生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides an elicitor composition using yew cells to produce paclitaxel, so as to solve the technical problem of low paclitaxel secretion in the yew cell culture process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] An inducer composition for producing paclitaxel by yew cells, which consists of the following ingredients in parts by weight: 8 parts of phenylalanine, 4 parts of sodium acetate, 2 parts of methyl jasmonate, 1 part of salicylic acid, and 1 part of silver nitrate 1 part of arachidonic acid, 1 part of ethylenediaminetetraacetic acid, 1 part of sodium citrate, 1 part of calcium chloride, 1 part of boric acid, and 50 parts of water.

Embodiment 2

[0019] An elicitor composition using yew cells to produce paclitaxel, which consists of the following ingredients in parts by weight: 10 parts of phenylalanine, 6 parts of sodium acetate, 3 parts of methyl jasmonate, 2 parts of salicylic acid, and 2 parts of silver nitrate 2 parts, 2 parts of arachidonic acid, 2 parts of ethylenediaminetetraacetic acid, 2 parts of sodium citrate, 2 parts of calcium chloride, 2 parts of boric acid, 60 parts of water.

Embodiment 3

[0021] An elicitor composition using yew cells to produce paclitaxel, which consists of the following ingredients in parts by weight: 9 parts of phenylalanine, 5 parts of sodium acetate, 2.3 parts of methyl jasmonate, 1.8 parts of salicylic acid, and 1.5 parts of silver nitrate 1.6 parts of arachidonic acid, 1.7 parts of ethylenediaminetetraacetic acid, 1.8 parts of sodium citrate, 1.2 parts of calcium chloride, 1.8 parts of boric acid, and 55 parts of water.

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PUM

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Abstract

The invention provides elicitor composition for producing paclitaxel from Taxus chinensis cells. According to the elicitor composition, components including phenylanine, sodium acetate, methyl jasmonate, salicylic acid, silver nitrate, arachidonic acid, EDTA (ethylene diamine tetraacetic acid), sodium citrate, calcium chloride, boric acid and the like are selected preferably, the dosage and ratio of the components are designed reasonably, and the using method of the elicitor composition is that the composition is directly added to a cell culture fluid at the initial culture stage or after cells are in the stable culture stage. Experiments find that after addition of the elicitor composition, although the cell culture density cannot be directly increased, the yield of paclitaxel in the same biomass of cell culture liquid is increased significantly, which indicates that the elicitor composition has a certain influence on the metabolism characteristic of the Taxus chinensis cells. The outstanding technical effect is realized by innovative technical means, and the present status of mass production of the paclitaxel is expected to be improved comprehensively by the aid of the elicitor composition.

Description

technical field [0001] The invention relates to the technical field of cell engineering, in particular to an elicitor composition for producing paclitaxel by using yew cells. Background technique [0002] Paclitaxel is a diterpenoid extracted from the bark of the Taxus genus Taxus. It is a new type of microtubule stabilizer with unique anticancer activity. It is considered by the National Cancer Institute of the United States to be the most important progress in tumor chemotherapy in the past 15 to 20 years. As a second-line treatment drug for advanced ovarian cancer, it has been approved for marketing in more than 40 countries so far, and has shown encouraging curative effects in the treatment of breast cancer, lung cancer, leukemia, gastrointestinal cancer and vascular restenosis after interventional therapy. . Paclitaxel has limited its clinical application due to the lack of resources and low water solubility. [0003] Paclitaxel and its analogues only exist in the ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/02C12N5/04
CPCC12P17/02C12N5/04C12N2500/05C12N2500/30C12N2500/32
Inventor 马丹马超
Owner 天津市博爱生物药业有限公司
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