Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Zostera marina isolated inflorescence callus induction method

A technology of callus and eelgrass, which is applied in the field of induction of ex vivo inflorescence callus of eelweed, can solve problems such as patents and literatures that have not been reported, and achieve the effect of improving the surface disinfection effect and shortening the acquisition time

Inactive Publication Date: 2016-03-16
SHANDONG UNIV
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the present invention, the inflorescence of Z. eosinophila is used as an explant to induce callus in a short period of time. At present, no relevant patents and documents using this technical solution have been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 A kind of induction method of eelgrass callus, its implementation step comprises:

[0016] 1) Selection of explants: collect the flowering branches of Zinaria cerevisiae, take the healthy and young spathe inflorescences, scrape off the attached organisms on the surface, wash them in seawater, and transfer them to the ultra-clean workbench. Soak in hydrogen peroxide solution for 15-20 minutes, rinse with sterilized sea water for 3 times, peel off the bract leaves outside spathe inflorescences to obtain sterile inflorescences;

[0017] 2) Induction culture of callus: cut the sterile inflorescence into 3-5mm long explant fragments, inoculate them in the induction medium, culture them at 20°C in the dark, and transfer them every half month. After 28 days, the tissues on both sides of the inflorescence germinated and began to expand, and gradually formed callus, and the induction rate was as high as 34%;

[0018] 3) The improved MS medium is based on MS medium, ...

Embodiment 2

[0019] Example 2 A kind of method for inducing callus of eel algae, its implementation steps are:

[0020] 1) Selection of explants: Take healthy and young spathe inflorescences from the flower branches of Echinopsis euphratica, scrape off the attached organisms on the surface, wash them in sea water, transfer them to a clean bench, and use 10% hydrogen peroxide to clean them. Soak in the solution for 15-20 minutes, rinse with sterilized seawater for 3 times, and peel off the bract leaves outside the spathe inflorescence to obtain a sterile inflorescence;

[0021] 2) Induction culture of callus: cut the sterile inflorescence into 3-5mm long explant fragments, inoculate them in the induction medium, culture them at 20°C in the dark, and transfer the bottles once every half month. After 40 days, the tissues on both sides of the inflorescence sprouted and began to expand, gradually forming callus, and the induction rate was 26%;

[0022] 3) The improved MS medium is based on M...

Embodiment 3

[0023] Embodiment 3: a kind of induction method of eosinopsis somatic embryo, its implementation steps are:

[0024] 1) Selection of explants: Take healthy and young spathe inflorescences from the flower branches of Echinopsis euphratica, scrape off the attached organisms on the surface, wash them in sea water, transfer them to a clean bench, and use 10% hydrogen peroxide to clean them. Soak in the solution for 15-20 minutes, rinse with sterilized seawater for 3 times, and peel off the bract leaves outside the spathe inflorescence to obtain a sterile inflorescence;

[0025] 2) Induction culture of callus: cut the sterile inflorescence into 3-5mm long explant fragments, inoculate them in the induction medium, culture them at 20°C in the dark, and transfer them every half month. After 40 days, the tissues on both sides of the inflorescence germinated, began to expand, and callus gradually formed, with an induction rate of 7%;

[0026] 3) The improved MS medium is based on MS me...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a zostera marina callus induction method, which comprises zostera marina inflorescence is adopted as an explant, the surface-sterilized inflorescence is cut into the material with a length of 3-5 mm, the obtained material is inoculated into a callus induction culture medium, callus induction is performed, transfer inoculation is performed every two weeks, both sides of the floral axis expand after culture for near 1 month, and the callus is gradually formed, wherein the callus induction culture medium adopts an improved MS culture medium as a base culture medium and is added with 2,4-D, casein hydrolyzate, sucrose, agar and sea salt. According to the present invention, the improved MS culture medium is used, such that the zostera marina callus induction can be effectively promoted, and the important technical support is provided for the production and the rapid propagation of the zostera marina tissue culture regeneration seedlings.

Description

technical field [0001] The invention belongs to the field of plant tissue culture research, and the invention relates to a method for inducing callus of isolated inflorescences of eelgrass. Background technique [0002] The induction of callus is the most important intermediate link in plant tissue culture technology. The regeneration of sterile plantlets of most plants needs to undergo the intermediate link of callus induction. Therefore, the successful induction of callus is the key to the regeneration of sterile plantlets At the same time, cultivating callus can also become a way for the rapid production of plant secondary metabolites. [0003] Eelweed ( Zostera marina L.) belongs to the genus Zosteraceae (Zosteraceae) ( Zostera ), widely distributed in shallow waters in the temperate zone of the northern hemisphere such as bays, lagoons and river estuaries, and widely distributed in the coastal waters of Liaoning, Hebei and Shandong in my country. But in the past cen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 郑凤英张飞刘雪芹金艳梅张伟韩晓弟
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com